Rare Association: Chagas' Disease and Hypertrophic Cardiomyopathy

Pastore, Carlos Alberto; Samesima, Nelson; Filho, Horácio Gomes Pereira; Varoni, Leonardo Paschoal Camacho; Rochitte, Carlos Eduardo; Vieira, Marcelo Luiz Campos; Ávila, Luiz Francisco Rodrigues de; Melo, Rodrigo de Jesus Louzeiro; Pereira, Alexandre da Costa; Daheer, Julia; M.D., Carlos Henrique del Carlo

doi:10.1111/anec.12229

Cited by 2 publications

(2 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compared to the main findings on EKG reported in the chronic phase of canine Chagas disease, such as right bundle branch block, left fascicular block, ventricular premature complex, ST-T segment changes, abnormal Q waves, lowvoltage QRS complex, and electric axis deviation [9,65,66], it is possible to assert that immunotherapy does not prevent the presentation of these alterations since most of them were found in some individuals of the experimental groups; however, the reduction in the number of abnormalities and the number of individuals treated with the pBCSSP4 and pBCSP plasmids separately was evident. The alterations found in the EKG are consistent with those reported in other studies, where the most common findings in both dogs and humans are RBBB associated with left anterior-superior fascicular block followed by various degrees of atrioventricular block [67,68]. Respiratory sinus arrhythmia was the predominant rhythm during the assay period [68].…”

Section: Groupsupporting

confidence: 90%

DNA Vaccine Treatment in Dogs Experimentally Infected with Trypanosoma cruzi

Arce-Fonseca

Carbajal-Hernández

Lozano-Camacho

et al. 2020

Journal of Immunology Research

View full text Add to dashboard Cite

Chagas disease is a chronic and potentially lethal disorder caused by the parasite Trypanosoma cruzi, and an effective treatment has not been developed for chronic Chagas disease. The objective of this study was to determine the effectiveness of a therapeutic DNA vaccine containing T. cruzi genes in dogs with experimentally induced Chagas disease through clinical, pathological, and immunological analyses. Infection of Beagle dogs with the H8 T. cruzi strain was performed intraperitoneally with 3500 metacyclic trypomastigotes/kg body weight. Two weeks after infection, plasmid DNA immunotherapy was administered thrice at 15-day intervals. The clinical (physical and cabinet studies), immunological (antibody and cytokine profiles and lymphoproliferation), and macro- and microscopic pathological findings were described. A significant increase in IgG and cell proliferation was recorded after immunotherapy, and the highest stimulation index (3.02) was observed in dogs treated with the pBCSSP4 plasmid. The second treatment with both plasmids induced an increase in IL-1, and the third treatment with the pBCSSP4 plasmid induced an increase in IL-6. The pBCSP plasmid had a good Th1 response regulated by high levels of IFN-gamma and TNF-alpha, whereas the combination of the two plasmids did not have a synergistic effect. Electrocardiographic studies registered lower abnormalities and the lowest number of individuals with abnormalities in each group treated with the therapeutic vaccine. Echocardiograms showed that the pBCSSP4 plasmid immunotherapy preserved cardiac structure and function to a greater extent and prevented cardiomegaly. The two plasmids alone controlled the infection moderately by a reduction in the inflammatory infiltrates in heart tissue. The immunotherapy was able to reduce the magnitude of cardiac lesions and modulate the cellular immune response; the pBCSP treatment showed a clear Th1 response; and pBCSSP4 induced a balanced Th1/Th2 immune response that prevented severe cardiac involvement. The pBCSSP4 plasmid had a better effect on most of the parameters evaluated in this study; therefore, this plasmid can be considered an optional treatment against Chagas disease in naturally infected dogs.

show abstract

Section: Groupsupporting

confidence: 90%

DNA Vaccine Treatment in Dogs Experimentally Infected with Trypanosoma cruzi

Arce-Fonseca

Carbajal-Hernández

Lozano-Camacho

et al. 2020

Journal of Immunology Research

View full text Add to dashboard Cite

show abstract

“… 5 While arrhythmias commonly manifest in chronic patients with dilated cardiomyopathy, instances of hypertrophied cardiomyopathy have also been documented in Chagas disease. 108 , 109 Mitochondrial dysfunction promotes cardiac arrhythmias, 48 , 49 , 50 and our data demonstrate that P-ILA treatment in cardiomyocytes causes mitochondrial dysfunction and increases β-AR signaling. Our research further establishes elevated levels of ANP in the hearts of P-ILA-treated mice, adding another marker indicative of Chagas cardiomyopathy in both uninfected and infected mice.…”

Section: Discussionmentioning

confidence: 54%