Rare bacteriohopanepolyols as markers for an autotrophic, intra-aerobic methanotroph

Kool, D.M.; Talbot, Helen M.; Rush, Darci; Ettwig, Katharina F.; Damsté, Jaap S. Sinninghe

doi:10.1016/j.gca.2014.04.002

Cited by 33 publications

(26 citation statements)

References 70 publications

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“…The contribution of 3Me-BHT increases with distance offshore, particularly in the 1.6-2.0 g cm −3 fraction (Figure 8), suggesting 3Me-BHT may also have a marine origin in the GOM which may or may not include methanotrophic bacteria. Methylomirabilis oxyfera (Kool et al, 2014) is currently the only known non-thermophilic source organism for BHhexol (the only other confirmed source being Alicyclobacillus acidoterrestris;Řezanka et al, 2011), but its origin is less well constrained. BHhexol was also identified in a marine sponge (likely representing bacterial symbionts; Shatz et al, 2000), however, De Jonge et al (2016) suggested a terrestrial origin due to its near-shore presence in the Kara Sea as well as other shelf systems and soils.…”

Section: Aminopolyol Sourcesmentioning

confidence: 99%

Origin of Sedimentary BHPs Along a Mississippi River–Gulf of Mexico Export Transect: Insights From Spatial and Density Distributions

2019

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We investigated the origin of sedimentary bacteriohopanepolyols (BHPs) in low-density (<1.6 g cm −3), mesodensity (1.6-2.0 and 2.0-2.5 g cm −3) and high-density fractions (>2.5 g cm −3) as well as unfractionated bulk samples in a highly dynamic coastal setting along a transect spanning from the Mississippi Delta into the Gulf of Mexico (GOM). We observe selective partitioning of BHPs among density fractions both in total abundance and structural diversity. BHPs primarily accumulate in the low-density fraction at all sites. Good correlation with other particle properties [surface area-normalized organic carbon loadings (OC/SA), C/N ratios, 14 C values, and lignin phenol abundances] and the spatial distribution of absolute and relative BHP abundances suggests that a significant fraction of BHPs are terrestrially sourced and entrained in the OC pool that is stabilized on clay particles or associated with plant fragments. Only a small subset of BHPs seems to have a significant autochthonous origin at the station furthest offshore and they are associated with the low and mesodensity fractions. While provenance and hydrodynamic sorting of particles across the shelf seem to primarily determine BHP inventories along the transect, the samples also harbor an unusual diversity of aminofunctionalized BHPs. A subset of these BHPs appears to derive from aerobic methane oxidizing bacteria and are likely exported with particles from the coastal swamps in the Mississippi Delta. Other amino-functionalized BHPs seem to derive from aerobic ammonia oxidizing bacteria thriving in the nutrient-rich Mississippi River plume. We find no evidence for anaerobic ammonium oxidizing bacteria related to recurring seasonal hypoxia on the Louisiana Shelf.

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Section: Aminopolyol Sourcesmentioning

confidence: 99%

Origin of Sedimentary BHPs Along a Mississippi River–Gulf of Mexico Export Transect: Insights From Spatial and Density Distributions

2019

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“…The enriched δ 13 C values of the hopane derivatives compared to those of the higher plant triterpenoids support their microbial source, even if they have an oxygenated functionality in position C-3. Other potential sources for hopanoids, such as methanotrophs, which have been described as Sphagnum symbionts, are not supported by the enriched δ 13 C signature [79,86].…”

Section: Roñanzasmentioning

confidence: 97%

Significance of the High Abundance of Pentacyclic Triterpenyl and Hopenyl Acetates in Sphagnum Peat Bogs from Northern Spain

et al. 2019

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Global warming is expected to increase the rate of CH4 emission from acidic peatlands leading to an increased interest on its mechanisms of formation. The main routes are through the reduction of CO2 by molecular hydrogen and through the cleavage of acetate. A predominance of the former, a reaction which also competes with homoacetogenesis to form acetate, may enrich the media in acetate, which could potentially be incorporated in the peat molecular markers. Acetates of triterpenoid biomarkers have been identified in peats and lake sediments and related to the input of higher plants. Nevertheless, the acetyl derivatives are found in very low amounts in fresh plants and in much lower amount than other derivatives with alcohol or ketone functional groups. The dichloromethane/methanol extracts of Asturian peat bog profiles (North Spain) were analyzed using gas chromatography/mass spectrometry (GC/MS) and compound-specific-isotope-analysis (CSIA). They show abundance of acetates of compounds with oleanane, ursane, and lupane skeletons derived from higher plants and with hopane skeleton, which can be considered a characteristic of these peats. Two families of 3-oxyhopenyl acetates with -17(21)- and -22(29)- configurations were detected in the upper part of the peat profiles, having a δ13C isotopic composition enriched by 4‰ compared with that of higher plant triterpenoids, and similar to that of microorganism-derived regular hopanoids. Both the acetate and ketone derivatives with the oxygenated functionality at C-3 were generally present in a given extract and tended to accumulate at certain depth in the profiles and in specific levels. The widespread occurrence of acetyl-derivatives, their higher concentration in the deeper layers of the peat, the fact that the acetates correspond to different compound families of diverse source and the very low amount of acetates identified in Ericaceae-contributing to the peat compared to the alcohols suggest that they were formed in the peat under particularly favorable environmental conditions. We postulate that these conditions could have been the existence of a medium enriched in acetic acid produced by the dominance of hydrogenotrophic methanogenesis and/or homoacetogenesis over acetoclastic methanogenesis. This phenomenon that has been preferentially described in Sphagnum bogs at high latitudes, and in the deeper layers of peat, appears to be also present in the temperate peats of the Asturian coast.

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“…M. oxyfera was shown to synthesise characteristic BHP biomarkers: bacteriohopanehexol (BHP-hexol) and 3-Me-BHP-hexol ( Fig. 2; Kool et al, 2014). Although the significance of this process within the marine nitrogen cycle is currently unknown, intra-aerobic methanotrophy has recently been detected in marine OMZs, suggesting it may play a role in the nitrogen cycle of these environments (Padilla et al, 2016).…”

Section: Nitrite-reducing Methanotrophy Biomarkersmentioning

confidence: 99%

Lipids as paleomarkers to constrain the marine nitrogen cycle

Rush

Damsté

2017

Environmental Microbiology

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SummaryGlobal climate is, in part, regulated by the effect of microbial processes on biogeochemical cycling. The nitrogen cycle, in particular, is driven by microorganisms responsible for the fixation and loss of nitrogen, and the reduction‐oxidation transformations of bio‐available nitrogen. Within marine systems, nitrogen availability is often the limiting factor in the growth of autotrophic organisms, intrinsically linking the nitrogen and carbon cycles. In order to elucidate the state of these cycles in the past, and help envisage present and future variability, it is essential to understand the specific microbial processes responsible for transforming bio‐available nitrogen species. As most microorganisms are soft‐bodied and seldom leave behind physical fossils in the sedimentary record, recalcitrant lipid biomarkers are used to unravel microbial processes in the geological past. This review emphasises the recent advances in marine nitrogen cycle lipid biomarkers, underlines the missing links still needed to fully elucidate past shifts in this biogeochemically‐important cycle, and provides examples of biomarker applications in the geological past.

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Rare bacteriohopanepolyols as markers for an autotrophic, intra-aerobic methanotroph

Cited by 33 publications

References 70 publications

Origin of Sedimentary BHPs Along a Mississippi River–Gulf of Mexico Export Transect: Insights From Spatial and Density Distributions

Origin of Sedimentary BHPs Along a Mississippi River–Gulf of Mexico Export Transect: Insights From Spatial and Density Distributions

Significance of the High Abundance of Pentacyclic Triterpenyl and Hopenyl Acetates in Sphagnum Peat Bogs from Northern Spain

Lipids as paleomarkers to constrain the marine nitrogen cycle

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