This study had two aims. AIM1 was to determine the agreement between mid-thigh vastus lateralis (VL) cross-sectional area measured by ultrasound (mCSAUS) versus magnetic resonance imaging (mCSAMRI) at a single time point, and the ability of each to detect hypertrophic changes. AIM2 was to assess the relationships between pre-to-post training changes in thigh lean mass determined by DXA, VL mCSAUS, ultrasound-determined VL thickness (VLThick), and VL mean myofiber cross-sectional area (fCSA) with changes in VL mCSAMRI. Twelve untrained males (Age: 20±1 y, BMI: 26.9±5.4 kg/m2; n=12) engaged in a 10-week resistance training program (2x/week) where right mid-thigh images and VL biopsies were obtained prior to and 72-hours following the last training bout. Participants' VL mCSAMRI (p=0.005), DXA thigh lean mass (p=0.015), and VLThick (p=0.001) increased following training, whereas VL mCSAUS and fCSA did not. For AIM1, mCSAUS demonstrated excellent concordance (CCC = 0.830) with mCSAMRI, albeit mCSAUS values were systematically lower compared to mCSAMRI (mean bias: -2.29 cm2). Additionally, PRE-to-POST VL mCSA changes between techniques exhibited good agreement (CCC = 0.700; mean bias: -1.08 cm2). For AIM2, moderate, positive correlations existed for PRE-to-POST changes in VL mCSAMRI and DXA thigh lean mass (r=0.580, p=0.048), mCSAUS (r=0.622, p=0.031), and VLThick (r=0.520, p=0.080). A moderate, negative correlation existed between mCSAMRI and fCSA (r=-0.569, p=0.054). Our findings have multiple implications: i) resistance training-induced hypertrophy was dependent on the quantification method, ii) ultrasound-determined mCSA shows good agreement with MRI, and iii) tissue-level changes poorly agreed with mean fCSA changes and this requires further research.