2008
DOI: 10.1016/s0076-6879(07)00431-4
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Ras‐Driven Transformation of Human Nestin‐Positive Pancreatic Epithelial Cells

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Cited by 17 publications
(14 citation statements)
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“…To address this question, we determined the expression of APLP2 in a series of cell lines generated from the hTERTHPNE cell line, which has been well characterized and used previously in several pancreatic cancer studies (4246). The hTERT-HPNE cell line is an immortalized, but non-transformed, cell line derived from human pancreatic ductal epithelium (42,43), and with oncogene-transfected derivatives of this line it has been demonstrated that expression of the human papillomavirus E6 and E7 oncogenes (which block p53 and Rb function, respectively) and SV40 small t antigen (st) can permit mutant K-Ras transformation (44,45). With these cell lines we have been able to compare expression of full-length APLP2, GAG-modified APLP2 and APLP2 C-terminal fragments in transformed versus matched, non-transformed pancreatic cells.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…To address this question, we determined the expression of APLP2 in a series of cell lines generated from the hTERTHPNE cell line, which has been well characterized and used previously in several pancreatic cancer studies (4246). The hTERT-HPNE cell line is an immortalized, but non-transformed, cell line derived from human pancreatic ductal epithelium (42,43), and with oncogene-transfected derivatives of this line it has been demonstrated that expression of the human papillomavirus E6 and E7 oncogenes (which block p53 and Rb function, respectively) and SV40 small t antigen (st) can permit mutant K-Ras transformation (44,45). With these cell lines we have been able to compare expression of full-length APLP2, GAG-modified APLP2 and APLP2 C-terminal fragments in transformed versus matched, non-transformed pancreatic cells.…”
Section: Resultsmentioning
confidence: 99%
“…Anti-actin (bottom panel) was used as a loading control. (B) Western blots for APLP2 expression in hTERT-HPNE (pancreatic ductal cells expressing telomerase, indicated as parental), with the stable addition of oncogenes: human papilloma virus genes E6 and E7 (E6/E7), SV40 small t antigen (st) and/or mutant K-Ras (K-RasG12D) (44,45). Addition of all oncogenes to hTERT-HPNE results in transformation of the cell line.…”
Section: Figurementioning
confidence: 99%
“…Nonetheless, it is known that nestin-positive cells can be isolated from the human pancreas and can be transformed with oncogenic K-ras, 45 and that Nestin expression status in epithelial cell types can be upregulated by hypoxia, TGFbeta and during EMT. 46 Our findings thus raise the possibility that targeting the nestin-positive cells within the pancreatic tumor mass may improve the efficacy of current therapeutic strategies by inhibiting the motility of both cancer cells and oncogene-transformed progenitor cells.…”
Section: Discussionmentioning
confidence: 99%
“…MiaPaCa-2 cells were purchased from ATCC, Manassas, VA in January 2015, and L3.6pl cells were provided by Dr. Jose Riveno, University of Florida, Gainesville, Florida in 2013. Immortalized human pancreatic normal epithelial (HPNE) cells were obtained from Dr. Paul Campbell, University of North Carolina in 2013 (16). These cell lines are well characterized and tested at regular intervals using RT-PCR and mycoplasma kit.…”
Section: Methodsmentioning
confidence: 99%