Ras/MEK/ERK Up-regulation of the Fibroblast KCaChannel FIK Is a Common Mechanism for Basic Fibroblast Growth Factor and Transforming Growth Factor-β Suppression of Myogenesis

Peña, Teresa L.; Chen, Shu Hui; Konieczny, Stephen F.; Rane, Stanley G.

doi:10.1074/jbc.275.18.13677

Cited by 81 publications

(79 citation statements)

References 22 publications

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“…The SK3 protein is abundant in porcine coronary endothelial cells, and immunofluorescent labelling confirms that IK1 and SK3 are expressed at the plasmalemma of porcine coronary endothelial cells [21]. In addition, it is well documented that in a number of cell types such as lymphocytes [22] and fibroblasts [23,24], IK1 expression is highly variable during the different phases of cell proliferation. By contrast, in human colonic and cavernosal vascular endothelium, no IK1 immunoreactivity was detected [20].…”

Section: Discussionmentioning

confidence: 60%

Reduced expression of SK3 and IK1 channel proteins in the cavernous tissue of diabetic rats

Jun¹,

Jia²,

Xu³

et al. 2010

Asian J Androl

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The small (SK3) and intermediate (IK1) conductance calcium-activated potassium channels could have key roles in the endothelium-dependent hyperpolarization factor pathway, which is believed to contribute to normal penile erection function. We aimed to investigate the expression of SK3 and IK1 in diabetic rodents. The experimental diabetes model was induced in 8-week-old male Sprague-Dawley rats (250-300 g) by a single administration of streptozotocin. Both the diabetes mellitus group (DM group, n = 20) and the control group (NDM group, n = 10) were injected with a low dose of apomorphine to allow for the measurement and comparison of the corresponding penile erections. The mRNA and protein expression levels of SK3 and IK1 were measured by reverse transcription polymerase chain reaction and western blot, respectively. Erectile function was significantly decreased in the DM group compared with control group (P < 0.05). The mRNA and protein expression levels of SK3 and IK1 were reduced in the cavernous tissue of diabetic rats compared with the control group (P < 0.05). Diabetes inhibits mRNA and protein expression of both SK3 and IK1 in the cavernous tissue of diabetic rats. This could play a key role in the development of erectile dysfunction in diabetic rats.

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Section: Discussionmentioning

confidence: 60%

Reduced expression of SK3 and IK1 channel proteins in the cavernous tissue of diabetic rats

Jun¹,

Jia²,

Xu³

et al. 2010

Asian J Androl

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show abstract

“…This may be related to the observation that other measures of muscle differentiation appear overall inhibited. This hypothesis is suggested by the observation that TGFB2, a potent inhibitor of skeletal muscle differentiation (22), was up-regulated 9.2-fold, whereas CARM1, MADH3, and NFATC3, all inhibitors of the myogenin͞MyoD pathways, were underexpressed by 1.9-to 4.6-fold (Fig. 4A) (23-25).…”

Section: Discussionmentioning

confidence: 96%

Expression profiling reveals altered satellite cell numbers and glycolytic enzyme transcription in nemaline myopathy muscle

Sanoudou

Haslett

Kho

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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The nemaline myopathies (NMs) are a clinically and genetically heterogeneous group of disorders characterized by nemaline rods and skeletal muscle weakness. Mutations in five sarcomeric thin filament genes have been identified. However, the molecular consequences of these mutations are unknown. Using Affymetrix oligonucleotide microarrays, we have analyzed the expression patterns of >21,000 genes and expressed sequence tags in skeletal muscles of 12 NM patients and 21 controls. Multiple complementary approaches were used for data analysis, including geometric fold analysis, two-tailed unequal variance t test, hierarchical clustering, relevance network, and nearest-neighbor analysis. We report the identification of high satellite cell populations in NM and the significant down-regulation of transcripts for key enzymes of glucose and glycogen metabolism as well as a possible regulator of fatty acid metabolism, UCP3. Interestingly, transcript level changes of multiple genes suggest possible changes in Ca 2؉ homeostasis. The increased expression of multiple structural proteins was consistent with increased fibrosis. This comprehensive study of downstream molecular consequences of NM gene mutations provides insights in the cellular events leading to the NM phenotype.

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“…It has been shown that the expression of IKCa1 is upregulated in all primary pancreatic cancer tissues tested (Jager et al, 2004) and in response to mitogenic activation of the Ras/ERK signaling pathway (Pena et al, 2000). Furthermore, clotrimazole inhibits proliferation of human prostate cancer cell lines, such as LNCaP and PC-3 cells (Parihar et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Blockage of intermediate-conductance-Ca2+-activated K+ channels inhibits progression of human endometrial cancer

et al. 2007

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Ras/MEK/ERK Up-regulation of the Fibroblast KCaChannel FIK Is a Common Mechanism for Basic Fibroblast Growth Factor and Transforming Growth Factor-β Suppression of Myogenesis

Cited by 81 publications

References 22 publications

Reduced expression of SK3 and IK1 channel proteins in the cavernous tissue of diabetic rats

Reduced expression of SK3 and IK1 channel proteins in the cavernous tissue of diabetic rats

Expression profiling reveals altered satellite cell numbers and glycolytic enzyme transcription in nemaline myopathy muscle

Blockage of intermediate-conductance-Ca2+-activated K+ channels inhibits progression of human endometrial cancer

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