Raster-scan optoacoustic angiography reveals 3D microcirculatory changes during cuffed occlusion

Subochev, Pavel; Orlova, Anna; Smolina, Ekaterina; Kirillov, A.V.; Shakhova, Natalia M.; Turchin, Ilya V.

doi:10.1088/1612-202x/aa9f68

Cited by 17 publications

(10 citation statements)

References 17 publications

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“…Three characteristic spectral ranges around λ 1 = 580 nm, λ 2 = 895 nm, and λ 3 = 1100 nm were revealed. The existence of three distinct spectral ranges is ascribed to two competing physical phenomena, namely, (1) Stronger absorption by blood leads to stronger OA signals; (2) Stronger light attenuation in the brain leads to limited penetration depth of the probing optical radiation and diminished OA signals. Thus, for superficial depths where light attenuation is weak the optimal probing wavelength would correspond to the maximum of the blood absorption spectra, i.e.…”

Section: Resultsmentioning

confidence: 99%

“…5(b)). The simulations were performed for the three considered wavelengths and two levels of NEP corresponding to the most [2] and the least [31] sensitive PVDF detectors developed by our group. In a simulated image each A-scan was calculated in accordance with Eq.…”

Section: Resultsmentioning

confidence: 99%

“…Hemoglobin is the major bio-chrome allowing for high-contrast OA angiography with optical contrast and ultrasonic spatial resolution. The high potential of OA angiography was confirmed in a number of clinical trials involving skin [2] and breast [3] lesions as well as numerous studies on small animal models [4].…”

Section: Introductionmentioning

confidence: 84%

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Toward whole-brain in vivo optoacoustic angiography of rodents: modeling and experimental observations

Subochev¹,

Smolina²,

Сергеева³

et al. 2020

Biomed. Opt. Express

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Cerebrovascular imaging of rodents is one of the trending applications of optoacoustics aimed at studying brain activity and pathology. Imaging of deep brain structures is often hindered by sub-optimal arrangement of the light delivery and acoustic detection systems. In our work we revisit the physics behind opto-acoustic signal generation for theoretical evaluation of optimal laser wavelengths to perform cerebrovascular optoacoustic angiography of rodents beyond the penetration barriers imposed by light diffusion in highly scattering and absorbing brain tissues. A comprehensive model based on diffusion approximation was developed to simulate optoacoustic signal generation using optical and acoustic parameters closely mimicking a typical murine brain. The model revealed three characteristic wavelength ranges in the visible and near-infrared spectra optimally suited for imaging cerebral vasculature of different size and depth. The theoretical conclusions are confirmed by numerical simulations while in vivo imaging experiments further validated the ability to accurately resolve brain vasculature at depths ranging between 0.7 and 7 mm.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Toward whole-brain in vivo optoacoustic angiography of rodents: modeling and experimental observations

Subochev¹,

Smolina²,

Сергеева³

et al. 2020

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

show abstract

“…Also, the dynamic aspects of occlusion and reperfusion of the skin vasculature could be visualized with OA systems operating at sufficiently high frame rates. 135,136…”

Section: Burns and Other Types Of Woundsmentioning

confidence: 99%

Optoacoustic imaging of the skin

Deán‐Ben

Razansky

2021

Experimental Dermatology

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Optoacoustic (OA, photoacoustic) imaging capitalizes on the synergistic combination of light excitation and ultrasound detection to empower biological and clinical investigations with rich optical contrast while effectively bridging the gap between micro and macroscopic imaging realms. State-of-the-art OA embodiments consistently provide images at micron-scale resolution through superficial tissue layers by means of focused illumination that can be smoothly exchanged for acoustic-resolution images at diffuse light depths of several millimetres to centimetres via ultrasound beamforming or tomographic reconstruction. Taken together, this unique multi-scale imaging capacity opens unprecedented capabilities for high-resolution in vivo interrogations of the skin at scalable depths. Moreover, diverse anatomical and functional information is retrieved via dynamic mapping of endogenous chromophores such as haemoglobin, melanin, lipids, collagen, water and others. This, along with the use of non-ionizing radiation, facilitates a clinical translation of the OA modalities. We review recent progress in OA imaging of the skin in preclinical and clinical studies exploiting the rich contrast provided by endogenous substances in tissues. The imaging capabilities of existing approaches are discussed in the context of initial translational studies on skin cancer, inflammatory skin diseases, wounds and other conditions.

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“…Thereby, OA may emerge as a powerful tool in dermatology for the assessment of a large variety of skin lesions, such as melanomas [22,152,153], psoriatic patches [23], and non-melanoma skin cancers [154], [155], [156], to name a few representative examples. High-resolution systems further enable visualizing superficial microvascular structures and analyzing microcirculatory changes [92,130,[157][158][159]. Angiographic imaging in deeper tissues may further enable assessing atherosclerotic plaques and stenosis in arteries [160], hemorrhages [161] or peripheral arterial disease [24].…”

Section: Perspectivementioning

confidence: 99%

Optoacoustic image formation approaches—a clinical perspective

Deán‐Ben

Razansky

2019

Phys. Med. Biol.

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Clinical translation of optoacoustic imaging is fostered by the rapid technical advances in imaging performance as well as the growing number of clinicians recognizing the immense diagnostic potential of this technology. Clinical optoacoustic systems are available in multiple configurations, including hand-held and endoscopic probes as well as raster-scan approaches. The hardware design must be adapted to the accessible portion of the imaged region and other application-specific requirements pertaining the achievable depth, field of view or spatio-temporal resolution. Equally important is the adequate choice of signal and image processing approach, which is largely responsible for the resulting imaging performance. Thus, new image reconstruction algorithms are constantly evolving in parallel to the newly-developed setups. This review focuses on recent progress on optoacoustic image formation algorithms and processing methods in the clinical setting. Major reconstruction challenges include real-time image rendering in two and three dimensions, efficient hybridization with other imaging modalitites as well as accurate interpretation and quantification of bio-markers, herein discussed in the context of ongoing progress in clinical translation. I.

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Raster-scan optoacoustic angiography reveals 3D microcirculatory changes during cuffed occlusion

Cited by 17 publications

References 17 publications

Toward whole-brain in vivo optoacoustic angiography of rodents: modeling and experimental observations

Toward whole-brain in vivo optoacoustic angiography of rodents: modeling and experimental observations

Optoacoustic imaging of the skin

Optoacoustic image formation approaches—a clinical perspective

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