2002
DOI: 10.1097/01.wcb.0000040944.89393.0f
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Rat Blood–Brain Barrier Genomics. II

Abstract: Summary:The tissue-specific gene expression at the brain microvasculature, which forms the blood-brain barrier (BBB) in vivo, can be elucidated with a brain vascular genomics program, which starts with the isolation of gene products derived from purified brain microvessels. Genes commonly expressed in peripheral organs are subtracted with the suppression subtractive hybridization method using driver cDNA produced from a pool of rat liver/kidney-derived poly A+RNA. From a screen of 480 clones in the subtracted … Show more

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Cited by 33 publications
(36 citation statements)
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“…14 Endothelial cellselective adhesion molecule (ESAM) is another protein that is localized to tight junctions, 15 and the SSH study indicated enhanced expression of this protein at the BBB. 11 The prevalence of growth factors, signal transduction molecules, and transcription factors extracted in the SSH screen support the hypothesis that the BBB aids in regulating brain plasticity and does so in an inductive manner through the release of growth factors [heparin…”
Section: Ssh Analysis Of the Bbb Transcriptomementioning
confidence: 88%
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“…14 Endothelial cellselective adhesion molecule (ESAM) is another protein that is localized to tight junctions, 15 and the SSH study indicated enhanced expression of this protein at the BBB. 11 The prevalence of growth factors, signal transduction molecules, and transcription factors extracted in the SSH screen support the hypothesis that the BBB aids in regulating brain plasticity and does so in an inductive manner through the release of growth factors [heparin…”
Section: Ssh Analysis Of the Bbb Transcriptomementioning
confidence: 88%
“…That is, the function of the protein that the gene encodes is unknown, and even putative functional assignment based on gene homology is rarely possible. In the three SSH case studies above, 45% (rat), 9 49% (rat), 11 and 47% (human) 3 of the genes that were identified to be BBB-enriched encoded proteins with unknown function. The fact that nearly 50% of the BBBenriched genes encode proteins with novel function is quite exciting.…”
Section: Identification Of Transcripts Encoding Proteins With Unknownmentioning
confidence: 99%
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