Rat bone marrow mesenchymal stem cells differentiate into hepatocytes<i>in vitro</i>

Kang, X.; Zang, Wei-Jin; Song, Taek Lyul; Xu, Xiaoli; Yu, Xin; Li, Dongling; Meng, Kewei; Wu, Shengli; Zhao, Zhiying

doi:10.3748/wjg.v11.i22.3479

Cited by 64 publications

(37 citation statements)

References 31 publications

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“…Schwartz et al [14] . and Kang et al [15] . reported that FGF-4 and HGF could induce differentiation of bone marrow MSCs into functional hepatocyte-like cells.…”

Section: The Extensive Characterization Of These Cells Has Revealedmentioning

confidence: 99%

“…It has been reported that bone marrowderived MSCs can differentiate into mesoderm cells such as osteoblasts [1][2][3] , adipocytes [4][5][6] , neural and brain cells [7][8][9][10] ,cardiomyocytes [11][12][13] and hepatocytes [14,15] in vivo or in vitro. However, it has been demonstrated that the number and the differentiating potential of bone marrow MSCs decrease with age [16].…”

Section: Introductionmentioning

confidence: 99%

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Fibroblast growth factor-4 and hepatocyte growth factor induce differentiation of human umbilical cord blood-derived mesenchymal stem cells into hepatocytes

Kang¹,

Zang²,

Bao³

et al. 2005

WJG

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“…Schwartz et al [14] . and Kang et al [15] . reported that FGF-4 and HGF could induce differentiation of bone marrow MSCs into functional hepatocyte-like cells.…”

Section: The Extensive Characterization Of These Cells Has Revealedmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fibroblast growth factor-4 and hepatocyte growth factor induce differentiation of human umbilical cord blood-derived mesenchymal stem cells into hepatocytes

Kang¹,

Zang²,

Bao³

et al. 2005

WJG

Self Cite

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show abstract

“…One medium (medium A) was supplemented with hepatocyte growth factor (HGF), a mesenchymally derived potent mitogen for parenchymal hepatocyte cells which acts via the HGF receptor c-MET, a transmembrane tyrosine kinase protein [Schaffler and Buchler, 2007]. As is well known, stem cells derived from human liver and rat bone marrow differentiate in mature hepatocytes when cultured in vitro in the presence of HGF and fibroblast growth factor 4, as indicated by the expression of functional cytochrome P450, albumin and urea production [Kang et al, 2005;Herrera et al, 2006]. In the second procedure, the me dium (medium B) was supplemented with oncostatin M (OSM), basic fibroblast growth factor (bFGF) and dimethyl sulfoxide (DMSO).…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study of Proliferation and Hepatic Differentiation of Human Adipose-Derived Stem Cells

Coradeghini

Guida

Scanarotti

et al. 2009

Cells Tissues Organs

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Human adipose-derived stem cells possess a lot of stem cell characteristics, so they may be considered a source of stem cell population. On the basis of that, we have investigated the hepatic potential of adipose-derived stem cells, obtained from liposuction, following two differentiation protocols. In the first procedure, medium was supplemented with epidermal growth factor (EGF), basic fibroblast growth factor, hepatocyte growth factor (HGF) and nicotinamide; the second involved the addition of factors such as dexametasone, EGF, insulin-transferrin-sodium selenite, HGF, dimethyl sulfoxide and oncostatin. In parallel, we carried out our study in the Hep G2 cell line, as human hepatic differentiated in vitro model. Immunocytochemical analysis and RT-PCR were performed using hepatic markers to evaluate cell differentiation. DNA content, MTT test and carboxyl fluorescein succinimidyl ester staining were carried out to evaluate cell proliferation. We reported the evidence of basal hepatic marker in undifferentiated adipose-derived stem cells, which confirmed their multipotency. A strong expression of albumin and α-fetoprotein was observed in hepatic-induced adipose-derived stem cells following both differentiation procedures. Morphological aspects of the two types of hepatic adipose-derived stem cells were alike. Proliferation index suggested that the first differentiation procedure promoted better growth than the second. These preliminary findings suggest adipose-derived stem cells may be induced into hepatic lineage, and the most significant difference between the two standard differentiation procedures concerns proliferation rate. This aspect is to be considered when adipose-derived stem cells are employed in research and clinical studies.

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“…Most of these approaches used toxic models, in which the intrinsic selfrenewing capacity of the recipient liver was suppressed (Dahlke, 2003, Kang, 2005. Di Bonzo observed only 0,2% human hepatocytes after transplantation of human MSC into immunodeficient mice with chronic liver injury (Di Bonzo, 2008).…”

Section: Functional Liver Remnant Volumementioning

confidence: 99%

Liver Parenchyma Regeneration in Connection with Extended Surgical Procedure - Experiment on Large Animal

Liška¹,

Třeška²,

Mírka³

et al. 2012

Liver Regeneration

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Rat bone marrow mesenchymal stem cells differentiate into hepatocytesin vitro

Abstract: The method combining gradient density centrifugation with plastic adherence can isolate MSCs. Rat MSCs may be differentiated into hepatocytes by FGF-4 and HGF. Cytokines may play a more important role in differentiation from rat MSCs into hepatocytes.

Cited by 64 publications

References 31 publications

Fibroblast growth factor-4 and hepatocyte growth factor induce differentiation of human umbilical cord blood-derived mesenchymal stem cells into hepatocytes

Fibroblast growth factor-4 and hepatocyte growth factor induce differentiation of human umbilical cord blood-derived mesenchymal stem cells into hepatocytes

A Comparative Study of Proliferation and Hepatic Differentiation of Human Adipose-Derived Stem Cells

Liver Parenchyma Regeneration in Connection with Extended Surgical Procedure - Experiment on Large Animal

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