Rat bone marrow mesenchymal stem cells induced by rrPDGF-BB promotes bone regeneration during distraction osteogenesis

Wu, Shuo; Zhang, Lijie; Zhang, Ruidan; Yang, Kang; Wei, Qin; Jia, Qiyu; Guo, Jian; Ma, Chuang

doi:10.3389/fbioe.2023.1110703

Cited by 5 publications

(3 citation statements)

References 58 publications

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“…Therefore, inducing osteogenesis promotion may be one of the most promising therapeutic approaches to treat osteolysis. BMSCs have the potential for osteogenic differentiation, which can promote bone formation and repair by differentiating into osteoblasts and osteocytes [51,52]. Therefore, the development of drugs that enhance the osteogenic differentiation capacity of BMSCs offers a new therapeutic approach for the treatment of osteolysis.…”

Section: Discussionmentioning

confidence: 99%

Carvacrol induces osteogenic differentiation of BMSCs and alleviates osteolysis in aged mice by upregulating lncRNA NEAT1 to promote SIRT1 expression

Xu,

Wang,

et al. 2023

APT

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Background: Wear particle-induced periprosthetic osteolysis is a major contributor to joint replacement failure and revision surgery in the elderly. Osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) is a promising approach for bone regeneration. Carvacrol may have bone-protective potential. This study investigated whether carvacrol alleviates osteolysis and promotes osteogenic differentiation of BM-SCs by regulating SIRT1 expression. Materials and Methods: An osteolysis model in aged mice was established by titanium (Ti) particle induction. BMSCs were isolated from femur and tibia of 18-month-old mice and cultured in osteogenic differentiation medium. RIP and RNA pull-down were used to evaluate the binding of SIRT1 and lncRNA NEAT1. Ubiquitination analysis was performed to investigate NEAT1-mediated regulation of SIRT1 ubiquitination modification levels. Results: Carvacrol treatment improved Ti particle-induced calvarial erosion and attenuated osteolysis. Carvacrol increased SIRT1 both in the mouse model of Ti-induced osteolysis and in BMSCs under osteogenic differentiation. Carvacrol treatment promoted ALP activity, bone mineralization capacity, and expression of osteogenic differentiation-related factors, whereas SIRT1 knockdown reversed this effect. LncRNA NEAT1 interacted with SIRT1, and overexpression of NEAT1 stabilized SIRT1 by inhibiting its ubiquitination modification. NEAT1 knockdown altered the promoting effect of carvacrol on osteogenic differentiation of BMSCs, while overexpression of SIRT1 reversed the effects of NEAT1 knockdown. Similarly, NEAT1 knockdown altered the inhibitory effect of carvacrol on osteolysis in vivo. Conclusion: Our research results demonstrate that carvacrol showed potential in treating osteolysis in aged mice by regulating NEAT1 to promote the expression of SIRT1 and can facilitate the osteogenic differentiation of BMSCs.

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Section: Discussionmentioning

confidence: 99%

Carvacrol induces osteogenic differentiation of BMSCs and alleviates osteolysis in aged mice by upregulating lncRNA NEAT1 to promote SIRT1 expression

Xu,

Wang,

et al. 2023

APT

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“…MSCs were isolated from the bone marrow of SD rats as described in a previous study. 15 Isolated cells were cultured in lowglucose Dulbecco's modified Eagle's medium (DMEM; Invitrogen) supplemented with 10 mM L-glutamine, 10% fetal bovine serum (FBS), 1% penicillin (100 U/mL), and streptomycin (100 mg/mL). Cells were incubated at 37 °C in a humidified atmosphere containing 5% carbon dioxide.…”

Section: Methodsmentioning

confidence: 99%

Effects of Different Surface Functionalizations of Silica Nanoparticles on Mesenchymal Stem Cells

Huang,

Zhou,

et al. 2024

ACS Appl. Bio Mater.

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Physicochemical properties of nanoparticles, such as particle size, surface charge, and particle shape, have a significant impact on cell activities. However, the effects of surface functionalization of nanoparticles with small chemical groups on stem cell behavior and function remain understudied. Herein, we incorporated different chemical functional groups (amino, DETA, hydroxyl, phosphate, and sulfonate with charges of +9.5, + 21.7, −14.1, −25.6, and −37.7, respectively) to the surface of inorganic silica nanoparticles. To trace their effects on mesenchymal stem cells (MSCs) of rat bone marrow, these functionalized silica nanoparticles were used to encapsulate Rhodamine B fluorophore dye. We found that surface functionalization with positively charged and short-chain chemical groups facilitates cell internalization and retention of nanoparticles in MSCs. The endocytic pathway differed among functionalized nanoparticles when tested with ion-channel inhibitors. Negatively charged nanoparticles mainly use lysosomal exocytosis to exit cells, while positively charged nanoparticles can undergo endosomal escape to avoid scavenging. The cytotoxic profiles of these functionalized silica nanoparticles are still within acceptable limits and tolerable. They exerted subtle effects on the actin cytoskeleton and migration ability. Last, phosphate-functionalized nanoparticles upregulate osteogenesis-related genes and induce osteoblast-like morphology, implying that it can direct MSCs lineage specification for bone tissue engineering. Our study provides insights into the rational design of biomaterials for effective drug delivery and regenerative medicine.

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“…LO2 cells were cultured in high-glucose DMEM containing 10% FBS. As previously reported, MSCs were extracted from the bone marrow of SD rats [ 26 ] and maintained in low-glucose DMEM containing 10% FBS. Cells between 3 and 5 passages were used.…”

Section: Methodsmentioning

confidence: 99%

Targeted Therapy of Acute Liver Injury via Cryptotanshinone-Loaded Biomimetic Nanoparticles Derived from Mesenchymal Stromal Cells Driven by Homing

Zhang,

Yi,

Jiang

et al. 2023

Pharmaceutics

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Acute liver injury (ALI) has the potential to compromise hepatic function rapidly, with severe cases posing a considerable threat to human health and wellbeing. Conventional treatments, such as the oral administration of antioxidants, can inadvertently lead to liver toxicity and other unwanted side effects. Mesenchymal stromal cells (MSCs) can target therapeutic agents directly to inflammatory sites owing to their homing effect, and they offer a promising avenue for the treatment of ALI. However, the efficacy and feasibility of these live cell products are hampered by challenges associated with delivery pathways and safety concerns. Therefore, in this work, MSC membranes were ingeniously harnessed as protective shells to encapsulate synthesized PLGA nanoparticle cores (PLGA/MSCs). This strategic approach enabled nanoparticles to simulate endogenous substances and yielded a core–shell nano-biomimetic structure. The biomimetic nanocarrier remarkably maintained the homing ability of MSCs to inflammatory sites. In this study, cryptotanshinone (CPT)-loaded PLGA/MSCs (CPT@PLGA/MSC) were prepared. These nanoparticles can be effectively internalized by LO2 cells. They reduced cellular oxidative stress and elevated inflammatory levels. In vivo results suggested that, after intravenous administration, CPT@PLGA/MSCs significantly reduced uptake by the reticuloendothelial system and immune recognition compared to PLGA nanoparticles without MSC membrane coatings, subsequently resulting in their targeted and enhanced accumulation in the liver. The effectiveness of CPT@PLGA/MSCs in alleviating carbon tetrachloride-induced oxidative stress and inflammation in a mouse model was unequivocally demonstrated through comprehensive histological examination and liver function tests. This study introduces a pioneering strategy with substantial potential for ALI treatment.

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Rat bone marrow mesenchymal stem cells induced by rrPDGF-BB promotes bone regeneration during distraction osteogenesis

Cited by 5 publications

References 58 publications

Carvacrol induces osteogenic differentiation of BMSCs and alleviates osteolysis in aged mice by upregulating lncRNA NEAT1 to promote SIRT1 expression

Carvacrol induces osteogenic differentiation of BMSCs and alleviates osteolysis in aged mice by upregulating lncRNA NEAT1 to promote SIRT1 expression

Effects of Different Surface Functionalizations of Silica Nanoparticles on Mesenchymal Stem Cells

Targeted Therapy of Acute Liver Injury via Cryptotanshinone-Loaded Biomimetic Nanoparticles Derived from Mesenchymal Stromal Cells Driven by Homing

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