Rat interferon inhibits steroid‐inducible glycerol 3‐phosphate dehydrogenase synthesis in a rat glial cell line

Illinger, Dominique; Coupin, G.; Richards, Mary H.; Poindron, Philippe

doi:10.1016/0014-5793(76)80334-1

Cited by 24 publications

(8 citation statements)

References 13 publications

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“…In virus-infected cells, it appears that they can act at different stages of the virus replicative cycle, depending on the virus and cell type [29], with inhibition of translation of viral mRNAs into proteins being the major effect in many cases [29]. In uninfected cells, in contrast, interferons have no effect on overall RNA and protein synthesis although synthesis of specific proteins may be enhanced or suppressed [2,4,5]. We have shown here that lymphoblastoid interferons have a selective effect on the uptake and metabolism of exogenous thymidine and its incorporation into DNA.…”

Section: Concluding Commentsmentioning

confidence: 99%

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Inhibition of Cell Division by Interferons

Gewert

Shah

Clemens

1981

European Journal of Biochemistry

107

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The Daudi line of human lymphoblastoid cells shows a high sensitivity towards growth inhibition by human interferons. In cells pretreated with 70 reference units/ml of an interferon preparation for 48 h, the incorporation of exogenous [3H]thymidine into DNA is inhibited by as much as 85%. We are investigating the extent to which this effect reflects a true inhibition of the rate of DNA synthesis or whether it may be caused by changes in the metabolic utilization of exogenous thymidine by the cells. lnterferon treatment results in a 30% inhibition of the rate of membrane transport and a 60% decrease in the rate of phosphorylation of [3H]thymidine in vivo. The latter effect is due to a decrease in V of thymidine kinase without any change in the value of K,,, for this enzyme. In addition to these changes, incorporation of [3H]uridine into DNA, which occurs as a result of the intracellular conversion of this precursor into thymidine nucleotides, is also inhibited by 75 x, whereas RNA labelling by [3H]uridine is decreased by only 15 in interferon-treated cells. Thus several different metabolic events associated with thymidine nucleotide metabolism and DNA synthesis in Daudi cells are disrupted by interferon treatment.

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Section: Concluding Commentsmentioning

confidence: 99%

“…In interferon-treated, uninfected cells, no overall change in cellular RNA and protein synthesis has been noted [2,3], although the synthesis of specific proteins may be enhanced or suppressed following interferon treatment [2,4,5]. In many cell types, however, interferons inhibit the incorporation of exogenous thymidine into DNA [6-81.…”

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confidence: 99%

Inhibition of Cell Division by Interferons

Gewert

Shah

Clemens

1981

European Journal of Biochemistry

107

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“…IFNs are naturally produced inhibitors of cell growth that can also affect differentiated cell function [38]. IFNs have been previously shown to inhibit the hormone-induced synthesis of several enzymes [3,19,32] and the differentiation and proliferation of glial cells [1,33]. Researchers investigated the effect of IFN-alpha/beta on the expression of glycerol phosphate dehydrogenase (GPDH) in both C6 glioma cells and pure cultures of oligodendrocytes.…”

Section: Discussionmentioning

confidence: 99%

The potential teratogenic effects of interferon beta-1a and interferon beta-1b on in vitro embryonic development

et al. 2016

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“…Lundblad and Lundgren have recently reported that interferon inhibited the proliferation of human glioma U-251 MG cells through a mechanism which prematurely stopped DNA synthesis (16). In addition to its antiproliferative activity, interferon affected a macromolecular synthesis resulting in either the inhibition or enhancement of its production (7,10,14,23). It may be postulated that interferon inhibits the synthesis of macromolecules related to cell division and enhances synthesis related to cell differentiation (8,9).…”

Section: Introductionmentioning

confidence: 99%

Interferon effects on multiplication, cytoplasmic protein and GFAP content, and morphology in human glioma cells

et al. 1983

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Beta-type human fibroblast interferon inhibited the multiplication of human glioma cells in a dose-dependent manner. The inhibitory effects were thought to be due to suppression of the cells entering the S-phase. After interferon treatment with 5 X 10(3) IU/ml for ten days, the mean cell volume, the soluble protein, and the glial fibrillary acidic protein increased to 970%, 190%, and 380% that of the controls, respectively. The interferon-treated cells changed shape and resembled mature astrocytes. It is presumed that beta-type human fibroblast interferon inhibits DNA synthesis of cultured glial cells and subsequently induces specific differentiated proteins and morphologic alteration.

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Rat interferon inhibits steroid‐inducible glycerol 3‐phosphate dehydrogenase synthesis in a rat glial cell line

Cited by 24 publications

References 13 publications

Inhibition of Cell Division by Interferons

Inhibition of Cell Division by Interferons

The potential teratogenic effects of interferon beta-1a and interferon beta-1b on in vitro embryonic development

Interferon effects on multiplication, cytoplasmic protein and GFAP content, and morphology in human glioma cells

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