Rat Liver Regeneration After 90% Partial Hepatectomy

Abstract

In previous studies, 90% partial hepatectomy in the rat was invariably accompanied by 100% mortality within 40 hr. This paper describes a technique by which 90% of the liver mass can be removed with only 14% mortality, provided that rats have free access to glucose-containing drinking water. Measurements of total liver DNA, [3H]thymidine labeling index and mitotic index suggest rapid cell proliferation, commensurate with a powerful regenerative stimulus. In the presence of normoglycemia, peripheral insulin and glucagon concentrations were elevated to levels normally observed in the portal vein.

Activin or Follistatin: Which is More Beneficial to Support Liver Regeneration After Massive Hepatectomy?

“…All animals were fasted for 24 h prior to surgery [12]. Ninety percent hepatectomy was performed through a midline incision under diethylether anesthesia as described by Gaub and Iverson [13]. After the hepatectomy, 50 mg of cefazol sodium was administered intraabdominally.…”

The Outcome of Major Hepatectomies Following Different Durations of Portal Vein Ligation in Rats

“…Regenerative response has not been occured because of the relatively small resection of the liver tissue in GII, in our study. Regenerative response might have been observed if PVL duration had been shorter as in Chjiiwa's study (32).…”

“…Dextrose effects regeneration by increasing the secretion of hepatotrophic insulin (29). Similarly in Gaub and Iverson's feeding rats model study, survive of the rats was increased to 80% with postoperative glucose solution (32).…”

“…It has been known that vena portae carries hepatotrophic factors (Insulin, glucagon etc.) (15,(27)(28)(29)(30)(31)(32). Atrophy in ligated lobe and hypertrophy in opposite lobe have been observed in human and animals and this was called Atrophy/Hypertrophy complex (AHC) (14).…”

Gene expression of ATP-binding cassette transporters during liver regeneration after 90% hepatectomy in rats

“…The rats received standard laboratory chow, had free access to food and water and were kept in a thermostatically controlled room (25˚C) with a 12-h light-dark cycle. Before undergoing the surgical procedures, all rats were fasted for 24 h. After the surgical procedures, they had free access to a 200 g/l glucose solution for 24 h to avoid post-operative hypoglycemia after the massive hepatectomy (23). This study was carried out in accordance with the Guidelines for Animal Experimentation, Hirosaki University, and all of the animals received humane care according to the criteria outlined in the 'Guide for the care and use of laboratory animals' prepared by the National Academy of Sciences and published by the National Institutes of Health (NIH publication 86-23, revised 1985).…”