Rat-Rat Hybridoma Formation and Rat Monoclonal Antibodies

Review: Veterinary Sources of Nonrodent Monoclonal Antibodies: Interspecific and Intraspecific Hybridomas

“…(27) As potential substitutes for human antibodies, and as model system s for the development of hum an fusions, primates have also received much attention, and antibodies have been developed in chimpanzees, (28)(29)(30) baboons, (28) and several species of macaque monkey. (28,(31)(32)(33)(34) The motivation for developing rat MAbs, (35) and to some extent hamster MAbs, (3 6,37) has been primarily the search for antibodies to endogenous murine antigens or antigens that produce poor responses in mice. The production of MAbs from rabbits (38)(39)(40)(41) and guinea pigs (42 ,43) has proved to be considerably more difficult, and, although som e immunoglobu lin fragment and intact rabbit antibodies have been reported from interspecific fusions, a most significant advance has been the development of a rabbit plasmacytoma fusion partner.…”

Performance of thiophilic adsorption chromatography in the purification of rat IgG2b monoclonal antibodies from serum- and protein-free culture supernatants

“…These antibodies are usually purified by affinity chromatography, either using protein A or G, or by immunoaffinity by means of mouse anti-rat immunoglobulin chain(s) (Harlow and Lane, 1988a;Bazin et al, 1990a;Darby et al, 1993;Savelkoul et al, 1994;Latinne et al, 1996;Soares et al, 1996;Xu et al, 1996;Andrew and Titus, 1997). These procedures, while rendering antibody preparations pure, require rather expensive materials; further, rat IgG2b does not bind to protein A and binds weakly to protein G (Harlow and Lane, 1988b;Andrew and Titus, 1997).…”

“…Other alternative purification methods are multi-step, lead to relatively pure preparations and are much less frequently applied (Harlow and Lane, 1988a;Bazin et al, 1990a).…”

Modern Concepts in Penicillium and Aspergillus Classification

“…Applied biologists are becoming increasingly frustrated and impatient, often refusing to accept changes (Bennett, 1985;Rossmore, 1988), whereas taxonomists regard any mention of restriction on name changes as a threat to their right to undertake objective scientific research and publish the results. With increased emphasis on the relevance of research to practical applications, systematic work which leads to instability in the vocabulary of applied biology finds difficulty in obtaining support.…”

“…The galactomannans represent one of the most widely distributed classes of polysaccharides among fungi and have been described as integral components of the mycelial cell wall (Johnston, 1965) as well as exocellular extracts (Gorin and Spencer, 1968). The majority of studies on the structure and antigenic properties of galactomannans have involved polysaccharides which have been either chemically extracted from cell wall preparations of mycelium or spores (Barreto-Bergter and Travassos, 1980;Barreto-Bergter et al, 1981;Webster and McGinley, 1980), or from cytoplasmic material after total disruption of the mycelium (Bennett et al, 1985). Galactomannans, with molecular weights greater than 10,000 daltons, can be also released during growth (Preston et al, 1969;Gander et al, 1974;Notermans et al, 1987).…”