Rat sciatic nerve regeneration in semipermeable artificial tubes

Hurtado, Hernán; Knoops, Bernard; Aguilar, P van den Bosch de

doi:10.1016/0014-4886(87)90133-6

Cited by 20 publications

(15 citation statements)

References 10 publications

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“…This results may go against the control side results of silicone tubes without stem cells which also went with other studies results regarding silicone tubes [19,30,35] which usually contains more fibrous and inflammatory cells and it can be correlated with the gap distance comparing this study to other studies which used much less gap distances. My control.…”

Section: Discussionsupporting

confidence: 47%

Angiogenesis induced by autologous whole bone marrow stem cells seeded on collagen scaffolds in silicone nerve tubes. An experimental study

Azeem

Al-Ahmady²,

Rahman

et al. 2014

Tanta Dental Journal

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Objective: This study aimed at evaluating Vascularity of tissues formed in silicone nerve tubes with collagen scaffolds seeded with Bone marrow stem cells (BMSC) as a sign of tissue regeneration. Materials and methods: Six adult mongrel dogs were included in this study; a 3 cm gap was made on the buccal branch of right side of facial nerve and also on the left side as a "control side". Next mononuclear cells containing mesenchymal stem cells were separated from whole bone marrow using Gradient separation method with Ficoll. Silicone tubes embedded with collagen type I sponge seeded with stem cells and neurogenic media were sutured in the perineural fascia of both ends of the cut nerve on the experimental side, while in the control side empty silicone tubes alone were sutured to the perineural fascia of the cut nerves. Healing was evaluated at 8 and 10 weeks scarifying 3 dogs each time and performing histological analysis. Results: After 8 weeks the 6 dogs showed limited movement in the facial area supplied by the buccal nerve on the experimental side and the salivary drooling decreased moderately. After 10 weeks the remaining dogs showed observable muscle movements and decreased salivary drooling in contrast to control side. These results go with the histological examination results showing massive multiple blood vessels formation and nerve like structures with partial absorption of the scaffold at 8 weeks then well organized nerve like tissues with dilated multiple blood vessels at 10 weeks. Conclusion: Collagen scaffolds seeded with mononuclear layer containing stem cells and nerve growth factors showed efficacy at achieving good neo-vascularization which lead to eventual good healing.

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Section: Discussionsupporting

confidence: 47%

Angiogenesis induced by autologous whole bone marrow stem cells seeded on collagen scaffolds in silicone nerve tubes. An experimental study

Azeem

Al-Ahmady²,

Rahman

et al. 2014

Tanta Dental Journal

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“…Esta estructura es similar a otras ya descritas en la literatura utilizando este sistema experimental (6,8,9). La matriz presenta un aspecto difuso y contiene esencialmente células sanguíneas y fibrina.…”

Section: Resultsunclassified

“…El modelo de la entubación se ha convertido en una herramienta experimental muy útil para el estudio de los fenómenos básicos subyacentes en la regeneración del nervio periférico (1-3). Las guías tubulares que se han utilizado son hechas de diversos materiales y permeabilidades: sintéticas impermeables, semipermeables o totalmente permeables; biodegradables o de origen biológico (4)(5)(6)(7)(8)(9). En la rata, los tubos de silicona impermeable permiten la regeneración de un mayor número de fibras mielinicas que los tubos acrilicos semipermeables (10).…”

Section: Discussionunclassified

“…Cuatro semanas de supervivencia: para los tres grupos experimentales, la organización histológica básica es similar a otras descripciones ya publicadas (6,9); en términos generales, se observa un puente circular de tejido conectivo, altamente vascularizado y colonizado por células de Schwann y fibras nerviosas mielínicas (figura 4A). Sin embargo, nuevamente se observa una diferencia importante entre los tubos con 9 agujeros y los otros.…”

Section: Su Madurez Depende Del Número De Agujerosunclassified

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Efecto de la permeabilidad en la regeneración del nervio ciático entubado de rata

Hurtado¹

1997

biomedica

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“…Los materiales de estas cámaras pueden ser diversos y van desde la utilización de materiales biológicos como venas, vainas mesoteliales (1 O), vainas perineurales (1 l ) , tubos de colágeno (12-13) y materiales sintéticos como silicona (14), ácido poli-D-láctico (S), acrílicos (15), polisulfonas y gore-tex, entre otros (4). Estudios recientes realizados con materiales sintéticos sugieren que las características físicas, químicas y eléctricas, así como la inclusión de geles dentro de estos canales, pueden generar cambios en el microambiente que optimizan los procesos de regeneración (1,16).…”

Section: Introductionunclassified

Estudio de la regeneración de nervio periférico a través de cámaras permeables de silicona con poros de 10 y 60 micras

Alzate

Sutachán²,

Duque³

et al. 1999

biomedica

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ResumenSe entubó el nervio ciático de ratas, luego de un corte transverso, utilizando una cámara de silicona de 14 mm de largo que tenía dos ventanas de 8 mm de largo cubiertas con malla de 10 o 60 pm. Después de 6 meses, los animales se perfundieron con una mezcla de glutaraldehído y paraformaldehído, y se evaluó el aspecto microscópico de los nervios regenerados en dos niveles dentro de la cámara (3 mm y nivel medio) y 5 mm distal a ésta. Se contó el número de fibras mielínicas presentes en cada nivel y tratamiento, y se realizó una comparación entre ellos. Se encontró una importante desorganización del endoneurio a nivel medio de la cámara. Distalmente, los conteos de fibras mielínicas fueron muy bajos. No se observaron diferencias significativas entre los grupos con mallas de 10 y 60 pm. En conclusión, la presencia de poros de 10 o 60 pm no impide la entrada masiva de fibroblastos a la cámara, llevando a una marcada desorganización del nervio regenerado.Palabras clave: regeneración, nervio periférico, entubación, fibroblasto, permeabilidad.A study of peripheral nerve regeneration in permeable silicone chambers with 10 and 60 micrometer pores Abstract Rat sciatic nerves were transected and intubated with a fourteen millimeter lengthsilicone chamber, with two 8 mm windows covered with either a 10 or 60 pm mesh. After six months, the animals were perfused with a glutaraldehyde and paraformaldehyde mixture and the regenerated nerve histology was evaluated at two levels within the chamber (3 mm and midlevel) and 5 mm distal to the chamber. The myelinated nerve fibers were counted and compared for al1 levels and treatments. We found a disorganized endoneurium at midchamber level. Distally, a very low myelinated nerve fibers count was registered. No significant differences were observed between 10 and 60 vm meshes.We conclude that 10 or 60 pm meshes do not control the massive invasion of the chamber by fibroblasts, leading to a highly disorganized regenerated nerve.

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Rat sciatic nerve regeneration in semipermeable artificial tubes

Cited by 20 publications

References 10 publications

Angiogenesis induced by autologous whole bone marrow stem cells seeded on collagen scaffolds in silicone nerve tubes. An experimental study

Angiogenesis induced by autologous whole bone marrow stem cells seeded on collagen scaffolds in silicone nerve tubes. An experimental study

Efecto de la permeabilidad en la regeneración del nervio ciático entubado de rata

Estudio de la regeneración de nervio periférico a través de cámaras permeables de silicona con poros de 10 y 60 micras

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