Rat sciatic nerve regeneration through a micromachined silicon chip

Zhao, Qing; Drott, Johan; Laurell, Thomas; Wallman, Lars; Lindström, Kjell; Bjursten, Lars Magnus; Lundborg, Göran; Montelius, Lars; Danielsen, Nils

doi:10.1016/s0142-9612(96)00112-3

Cited by 61 publications

(30 citation statements)

References 21 publications

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“…As discussed above, initial implantation stability seems to be an important factor and, therefore, porous surfaces may have an advantage in long term applications. In the specific sieve electrode design, implant stability is usually achieved by enclosing the sieve electrode in a nerve regeneration chamber and securing the two nerve stumps the chamber wall [4], [6], [9], [11]. Unstable implant design relative to the ends of the transected nerve has been reported to induce damage to the nerve ends during the movements of the host [3], [55].…”

Section: A Discussionmentioning

confidence: 99%

“…Surfaces Made Out of Silicon and Titanium ation through sieve electrodes and to record nerve signals and not to study the tissue reactions evoked by the implanted material with a few exceptions [11]. This biomaterial aspect has to be considered since excessive fibrous encapsulation, which could hamper necessary electrochemical contact between axons and electrodes, has been suggested to be the most important reason for failure in the clinical use of neural interfaces [16].…”

Section: Tissue Reactions Evoked By Porous and Planementioning

confidence: 99%

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Tissue reactions evoked by porous and plane surfaces made out of silicon and titanium

Rosengren

Wallman

Danielsen

et al. 2002

IEEE Trans. Biomed. Eng.

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Tissue reactions evoked by porous and plane surfaces made out of silicon and titanium.Rosengren, Agneta; Wallman, Lars; Danielsen, Nils; Laurell, Thomas; Bjursten, Lars Magnus General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal Abstract-Square-shaped silicon or titanium implants with plane or porous surfaces surrounded by a rim of silicone were implanted in the rat abdominal wall for evaluation of the tissue response after one, six, or 12 weeks. Cell damage was identified as increased membrane permeability using fluorescence microscopy by injection of propidium iodide prior to the killing of the rats. Capsule thickness and immunohistochemical quantification of macrophages were used as a further measure of the foreign-body reaction. There were no significant differences in capsular cell densities for macrophages, total cells (macrophages, fibroblasts, and other cells), or necrotic cells at the different time points for the four surfaces studied. However, significant differences in the kinetics of the response were found between plane surfaces compared with porous ones. Both types of plane surfaces developed a significant increase in capsule thickness over time in contrast to the porous implants. Porous silicon displayed a significant decrease in total cells in the reactive capsule over time. Furthermore, porous silicon and titanium surfaces displayed a significant decrease in total cell numbers at the implant interface between six and 12 weeks. The present study demonstrated that implanted silicon elicited soft-tissue reactions comparable to that of titanium.

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Section: A Discussionmentioning

confidence: 99%

Section: Tissue Reactions Evoked By Porous and Planementioning

confidence: 99%

Tissue reactions evoked by porous and plane surfaces made out of silicon and titanium

Rosengren

Wallman

Danielsen

et al. 2002

IEEE Trans. Biomed. Eng.

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“…[41][42][43][44] The cells exhibit sensitivity to the dimensions of the microgrooves. 45 Most of the microgrooves have been fabricated on inorganic substrates, such as micromachined silicon chips 46 …”

Section: Oriented Matricesmentioning

confidence: 99%

Types of neural guides and using nanotechnology for peripheral nerve reconstruction

Biazar

Khorasani²,

Montazeri³

et al. 2010

IJN

103

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Peripheral nerve injuries can lead to lifetime loss of function and permanent disfigurement. Different methods, such as conventional allograft procedures and use of biologic tubes present problems when used for damaged peripheral nerve reconstruction. Designed scaffolds comprised of natural and synthetic materials are now widely used in the reconstruction of damaged tissues. Utilization of absorbable and nonabsorbable synthetic and natural polymers with unique characteristics can be an appropriate solution to repair damaged nerve tissues. Polymeric nanofibrous scaffolds with properties similar to neural structures can be more effective in the reconstruction process. Better cell adhesion and migration, more guiding of axons, and structural features, such as porosity, provide a clearer role for nanofibers in the restoration of neural tissues. In this paper, basic concepts of peripheral nerve injury, types of artificial and natural guides, and methods to improve the performance of tubes, such as orientation, nanotechnology applications for nerve reconstruction, fibers and nanofibers, electrospinning methods, and their application in peripheral nerve reconstruction are reviewed.

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“…16 Microstamping techniques can be used to pattern proteins on biodegradable surfaces, but cannot be used to create microgrooves on substrates for physical guidance. 17 We have developed novel patterning techniques to create microgrooves on biodegradable polymer substrates and attach adhesive proteins selectively in the grooves.…”

Section: Introduction Nmentioning

confidence: 99%

Synergistic Effects of Physical and Chemical Guidance Cues on Neurite Alignment and Outgrowth on Biodegradable Polymer Substrates

2002

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This article demonstrates that directional outgrowth of neurites is promoted by applying a combination of physical and chemical cues to biodegradable polymer substrates. Films of poly-D,L-lactic acid and poly(lactide-co-glycolide) were micropatterned to form grooves on substrate surfaces, using novel indirect transfer techniques developed specifically for biodegradable polymers that cannot be micropatterned directly. Laminin was selectively adsorbed in the grooves. Whole and dissociated dorsal root ganglia were seeded on the substrates and neurite outgrowth and alignment along the microgrooves were measured. The microgrooves provide physical guidance, whereas laminin provides chemical cues to the neurons. The groove depth and spacing were found to significantly influence neurite alignment. The presence of laminin was found to promote neurite adhesion and outgrowth along the grooves. Using a combination of optimized physical and chemical cues, excellent spatial control of directional neurite outgrowth, with up to 95% alignment of neurites, was obtained. The synergistic effect of physical and chemical guidance cues was found to be more effective than individual cues in promoting directional outgrowth of neurites. ABSTRACTThis article demonstrates that directional outgrowth of neurites is promoted by applying a combination of physical and chemical cues to biodegradable polymer substrates. Films of poly-D,L-lactic acid and poly(lactide-co-glycolide) were micropatterned to form grooves on substrate surfaces, using novel indirect transfer techniques developed specifically for biodegradable polymers that cannot be micropatterned directly. Laminin was selectively adsorbed in the grooves. Whole and dissociated dorsal root ganglia were seeded on the substrates and neurite outgrowth and alignment along the microgrooves were measured. The microgrooves provide physical guidance, whereas laminin provides chemical cues to the neurons. The groove depth and spacing were found to significantly influence neurite alignment. The presence of laminin was found to promote neurite adhesion and outgrowth along the grooves. Using a combination of optimized physical and chemical cues, excellent spatial control of directional neurite outgrowth, with up to 95% alignment of neurites, was obtained. The synergistic effect of physical and chemical guidance cues was found to be more effective than individual cues in promoting directional outgrowth of neurites.

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Rat sciatic nerve regeneration through a micromachined silicon chip

Cited by 61 publications

References 21 publications

Tissue reactions evoked by porous and plane surfaces made out of silicon and titanium

Tissue reactions evoked by porous and plane surfaces made out of silicon and titanium

Types of neural guides and using nanotechnology for peripheral nerve reconstruction

Synergistic Effects of Physical and Chemical Guidance Cues on Neurite Alignment and Outgrowth on Biodegradable Polymer Substrates

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