Rate of Healing in Skin-Grafted Burn Wounds

Jewell, Lisa; Guerrero, R.E. Asensio; Quesada, Abel R; Chan, Linda S.; Garner, W.L.

doi:10.1097/01.prs.0000267416.64164.7e

Cited by 44 publications

(29 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of all animals treated with an STSG, 80% showed a graft adherence of more than 75%; however, there were no significant differences between the treatment group receiving eCryo and the control. This is comparable with the results of a recent prospective clinical trial showing healing rates in skin grafted burn wounds (24). Only 46% of patients were completely healed after 2 weeks and critically ill patients were excluded from this study.…”

Section: Discussionsupporting

confidence: 87%

See 1 more Smart Citation

Novel cryoprecipitate for wound healing and skin grafts in rats

Scholz

Waltzman

Wirth

et al. 2008

International Wound Journal

View full text Add to dashboard Cite

The authors sought to evaluate the ability of locally administered enhanced cryoprecipitate (eCryo) to improve the wound healing of split thickness skin grafts (STSG) and their donor sites. An STSG (5 x 5 cm) was harvested on the back of 30 rats and divided into four areas that were then treated in one of the following groups: A: 'standard' dressing without STSG; B: eCryo without STSG; C: eCryo with STSG coverage and D: STSG alone. Macroscopic and histological assessments (histomorphometric grading scale and cellular composition) were evaluated at days 7, 14, 21 and 28 for wound healing. All wound beds as well as STSGs healed well without any complications. Eighty per cent of the STSG showed a histological graft take of >75% after 28 days. There were no statistically significant differences of macroscopic or histological results between the groups at any time point. Preparation of eCryo is easy and effective. Its use as an adhesive for STSGs is safe and shows similar results as controls. The theoretical benefits of eCryo did not show significant differences. Possible reasons as well as important findings for future research on wound healing are discussed.

show abstract

Section: Discussionsupporting

confidence: 87%

“…• our data show that local therapy with eCryo is an easy, quick and safe procedure, and that the results obtained here are comparable with the results achieved by the use of fibrin glues (24). Only 46% of patients were completely healed after 2 weeks and critically ill patients were excluded from this study.…”

Section: Key Pointssupporting

confidence: 74%

Novel cryoprecipitate for wound healing and skin grafts in rats

Scholz

Waltzman

Wirth

et al. 2008

International Wound Journal

View full text Add to dashboard Cite

show abstract

“…The integrity of this TCSA after reaching full differentiation could be maintained in vitro for up to 6 weeks. The time needed for complete wound closure under optimal conditions is between 2 and 75 days [42] for meshed split skin. TCSAs can reduce the healing time.…”

Section: Discussionmentioning

confidence: 99%

Clinical Application of a Tissue-Cultured Skin Autograft: An Alternative for the Treatment of Non-Healing or Slowly Healing Wounds?

Zöller¹,

Valesky²,

Butting³

et al. 2014

Dermatology

View full text Add to dashboard Cite

Background: The treatment regime of non-healing or slowly healing wounds is constantly improving. One aspect is surgical defect coverage whereby mesh grafts and keratinocyte suspension are applied. Objective: Tissue-cultured skin autografts may be an alternative for the treatment of full-thickness wounds and wounds that cover large areas of the body surface. Methods: Autologous epidermal and dermal cells were isolated, expanded in vitro and seeded on collagen-elastin scaffolds. The developed autograft was immunohistochemically characterized and subsequently transplanted onto a facial chronic ulceration of a 71-year-old patient with vulnerable atrophic skin. Results: Characterization of the skin equivalent revealed comparability to healthy human skin due to the epidermal strata, differentiation and proliferation markers. Within 138 days, the skin structure at the transplantation site closely correlated with the adjacent undisturbed skin. Conclusion: The present study demonstrates the comparability of the developed organotypic skin equivalent to healthy human skin and the versatility for clinical applications.

show abstract

“…Moreover, the transplanted keratinocytes facilitate the formation of blood vessels [8]. In addition, skin grafting has been used for the treatment of burns and chronic leg ulcers, which can promote faster re-epithelialization of partial thickness wounds [9][10][11][12]. However, the potential mechanism of keratinocytes for accelerated skin wound healing is unclear.…”

mentioning

confidence: 99%

Microenvironment-induced myofibroblast-like conversion of engrafted keratinocytes

Han

et al. 2014

Sci. China Life Sci.

View full text Add to dashboard Cite

Myofibroblasts, recognized classically by -smooth muscle actin (-SMA) expression, play a key role in the wound-healing process, promoting wound closure and matrix deposition. Although a body of evidence shows that keratinocytes explanted onto a wound bed promote closure of a skin injury, the underlying mechanisms are not well understood. The basal layer of epidermis is rich in undifferentiated keratinocytes (UKs). We showed that UKs injected into granulation tissue could switch into -SMA positive cells, and accelerate the rate of skin wound healing. In addition, when the epidermis sheets isolated from foreskin cover up the wound bed or are induced in vitro, keratinocytes located at the basal layers or adjacent sites were observed to convert into myofibroblast-like cells. Thus, UKs have a potential for myofibroblastic transition, which provides a novel mechanism by which keratinocyte explants accelerate skin wound healing. epithelial-mesenchymal transition, epithelial-myofibroblast transition, keratinocyte-myofibroblast transition, myofibroblast, wound healing Citation:Li MR, Ti DD, Han WD, Fu XB. Microenvironment-induced myofibroblast-like conversion of engrafted keratinocytes.

show abstract

Rate of Healing in Skin-Grafted Burn Wounds

Cited by 44 publications

References 13 publications

Novel cryoprecipitate for wound healing and skin grafts in rats

Novel cryoprecipitate for wound healing and skin grafts in rats

Clinical Application of a Tissue-Cultured Skin Autograft: An Alternative for the Treatment of Non-Healing or Slowly Healing Wounds?

Microenvironment-induced myofibroblast-like conversion of engrafted keratinocytes

Contact Info

Product

Resources

About