Rate of mosaicism unaffected by oxygen levels during incubation

Black, Margaret A.; Masbou, A.K.; McCulloh, David H.; McCaffrey, C.; Grifo, J.

doi:10.1016/j.fertnstert.2018.02.035

Cited by 2 publications

(1 citation statement)

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“…Culture was performed during different time periods in different incubators. Rates of mosaicism were high and differed, being around 4% lower in the low oxygen groups (37.3%) compared with the high oxygen (41.6%), but this was not statistically significant (Black et al, 2018). While unable to isolate the impact of oxygen levels alone, another preliminary study suggested that improper CO 2 and elevated O 2 (∼21%) may increase blastocyst mosaicism, resulting in an ∼17% higher rate of mosaicism (2.3% versus 20%) due to elevated pH and oxygen when compared with optimized culture conditions (Katz-Jaffe et al, 2018).…”

Section: Oxygenmentioning

confidence: 78%

Controversies in ART: can the IVF laboratory influence preimplantation embryo aneuploidy?

Swain

2019

Reproductive BioMedicine Online

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Section: Oxygenmentioning

confidence: 78%

Controversies in ART: can the IVF laboratory influence preimplantation embryo aneuploidy?

Swain

2019

Reproductive BioMedicine Online

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Consistency in rates of diagnosis of embryonic mosaicism, segmental abnormalities, and “no call” results among experienced embryologists performing preimplantation genetic testing for aneuploidy

et al. 2020

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Objective: To determine whether differences exist in rates of subchromosomal abnormalities, mosaicism, and ''no call'' results among embryologists performing and loading trophectoderm biopsies for preimplantation genetic testing for aneuploidy (PGT-A). Design: Retrospective cohort. Setting: Large infertility center. Patient(s): All patients undergoing in vitro fertilization with PGT-A. Intervention(s): The NexCCS next generation sequencing platform was used for PGT-A. The c 2 testing assessed differences in rates of primary outcomes between embryologists. Intraclass correlation coefficients evaluated inter-embryologist reliability in rates of abnormal and no call results. Median absolute performance difference (MAPD) scores, which quantify the impact of technical variation on analytical performance, were averaged for individual embryologists. Analysis of variance assessed differences in mean MAPD scores. Main Outcome Measure(s): Interoperator variability in rates of mosaic, segmental, and no call results. Result(s): Four embryologists performed 30,899 biopsies and 6 embryologists loaded specimens into designated tubes. Among individuals performing trophectoderm sampling, rates of mosaicism were 4.3% to 6.1%, segmental errors were 9.0% to 10.7%, and inconclusive results were 1.1% to 2.9%. For those loading, the incidence of mosaicism was 4.2% to 5.9%, subchromosomal abnormalities was 9.7% to 10.4%, and no call results was 1.2% to 2.2%. The intraclass correlation coefficient was 0.978 for embryologists performing biopsies and 0.981 for those loading. Differences in mean MAPD scores were within 0.6% and 0.2% of each other for doing biopsies and loading embryologists, respectively. Conclusion(s): Rates of mosaicism, segmental, and no call PGT-A results are consistent among experienced embryologists. Due to the large sample size included, differences within 1% of the mean were deemed clinically irrelevant despite statistical significance. (Fertil Steril Rep Ò 2020;1:119-24. Ó2020 by American Society for Reproductive Medicine.

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Rate of mosaicism unaffected by oxygen levels during incubation

Cited by 2 publications

References 0 publications

Controversies in ART: can the IVF laboratory influence preimplantation embryo aneuploidy?

Controversies in ART: can the IVF laboratory influence preimplantation embryo aneuploidy?

Consistency in rates of diagnosis of embryonic mosaicism, segmental abnormalities, and “no call” results among experienced embryologists performing preimplantation genetic testing for aneuploidy

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