Rate of spontaneous polyploidization in haploid yeast &lt;em&gt;Saccharomyces cerevisiae&lt;/em&gt;

Andreychuk, Yu. V.; Zhuk, Anna S.; Tarakhovskaya, Elena; Инге-Вечтомов, С. Г.; Stepchenkova, Elena I.

doi:10.21638/spbu03.2022.202

Cited by 2 publications

(1 citation statement)

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“…Laboratory yeast strains are usually heterothallic because they lack the HO endonuclease, which is involved in mating-type switching by introducing a break into the MAT locus [6] (Figure 1C,D, see Section 7 for more details). Spontaneous changes of ploidy in laboratory yeast strains are not rare and occur with a rate about 10 −5 -10 −4 (autodiploidization through whole-genome duplication) or lower (~10 −5 -10 −7 ) if it involves mating-type switching and hybridization [7,8]. Regulation of transcription of the mating-type specific genes in haploid and diploid cells of yeast S. cerevisiae (a simplified scheme).…”

Section: Introductionmentioning

confidence: 99%

Practical Approaches for the Yeast Saccharomyces cerevisiae Genome Modification

Stepchenkova,

Zadorsky,

Shumega

et al. 2023

IJMS

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The yeast S. cerevisiae is a unique genetic object for which a wide range of relatively simple, inexpensive, and non-time-consuming methods have been developed that allow the performing of a wide variety of genome modifications. Among the latter, one can mention point mutations, disruptions and deletions of particular genes and regions of chromosomes, insertion of cassettes for the expression of heterologous genes, targeted chromosomal rearrangements such as translocations and inversions, directed changes in the karyotype (loss or duplication of particular chromosomes, changes in the level of ploidy), mating-type changes, etc. Classical yeast genome manipulations have been advanced with CRISPR/Cas9 technology in recent years that allow for the generation of multiple simultaneous changes in the yeast genome. In this review we discuss practical applications of both the classical yeast genome modification methods as well as CRISPR/Cas9 technology. In addition, we review methods for ploidy changes, including aneuploid generation, methods for mating type switching and directed DSB. Combined with a description of useful selective markers and transformation techniques, this work represents a nearly complete guide to yeast genome modification.

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Section: Introductionmentioning

confidence: 99%

Practical Approaches for the Yeast Saccharomyces cerevisiae Genome Modification

Stepchenkova,

Zadorsky,

Shumega

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

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Development of a Robust Saccharomyces cerevisiae Strain for Efficient Co-Fermentation of Mixed Sugars and Enhanced Inhibitor Tolerance through Protoplast Fusion

Zhao,

et al. 2024

Microorganisms

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The economical and efficient commercial production of second-generation bioethanol requires fermentation microorganisms capable of entirely and rapidly utilizing all sugars in lignocellulosic hydrolysates. In this study, we developed a recombinant Saccharomyces cerevisiae strain, BLH510, through protoplast fusion and metabolic engineering to enhance its ability to co-ferment glucose, xylose, cellobiose, and xylooligosaccharides while tolerating various inhibitors commonly found in lignocellulosic hydrolysates. The parental strains, LF1 and BLN26, were selected for their superior glucose/xylose co-fermentation capabilities and inhibitor tolerance, respectively. The fusion strain BLH510 demonstrated efficient utilization of mixed sugars and high ethanol yield under oxygen-limited conditions. Under low inoculum conditions, strain BLH510 could completely consume all four kinds of sugars in the medium within 84 h. The fermentation produced 33.96 g/L ethanol, achieving 84.3% of the theoretical ethanol yield. Despite the challenging presence of mixed inhibitors, BLH510 successfully metabolized all four sugars above after 120 h of fermentation, producing approximately 30 g/L ethanol and reaching 83% of the theoretical yield. Also, strain BLH510 exhibited increased intracellular trehalose content, particularly under conditions with mixed inhibitors, where the intracellular trehalose reached 239.3 mg/g yeast biomass. This elevated trehalose content contributes to the enhanced stress tolerance of BLH510. The study also optimized conditions for protoplast preparation and fusion, balancing high preparation efficiency and satisfactory regeneration efficiency. The results indicate that BLH510 is a promising candidate for industrial second-generation bioethanol production from lignocellulosic biomass, offering improved performance under challenging fermentation conditions. Our work demonstrates the potential of combining protoplast fusion and metabolic engineering to develop superior S. cerevisiae strains for lignocellulosic bioethanol production. This approach can also be extended to develop robust microbial platforms for producing a wide array of lignocellulosic biomass-based biochemicals.

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Rate of spontaneous polyploidization in haploid yeast <em>Saccharomyces cerevisiae</em>

Cited by 2 publications

References 67 publications

Practical Approaches for the Yeast Saccharomyces cerevisiae Genome Modification

Practical Approaches for the Yeast Saccharomyces cerevisiae Genome Modification

Development of a Robust Saccharomyces cerevisiae Strain for Efficient Co-Fermentation of Mixed Sugars and Enhanced Inhibitor Tolerance through Protoplast Fusion

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