Rates of corolla growth in tobacco determined with the plastochron index

Abstract

The growth of vegetative and reproductive shoots of Nicotiana tabacum L. cv. Xanthi is analyzed with the plastochron index to estimate the relationship between corolla growth and time. The plastochron of leaves 9 through 20 declines steadily at each successive node. The flower plastochron increases steadily during the growth of an individual cyme, with the most distal flower to open having the longest plastochron. Variation in the flower plastochron is the result of variation in the rate of flower initiation, not the growth rate of individual flowers. The corolla has an extended phase of approximately constant relative growth in length (between 0.2 · d(-1) and 0.3 · d(-1)) until a peak of growth (0.5 · d(-1)) 2-3 d before anthesis. Corollas also have periodic peaks and troughs of growth that are low in amplitude (0.1 · d(-1)), but persist throughout most of corolla development. The pattern of corolla expansion contrasts strongly with earlier reports of the pattern of tobacco leaf growth.

A proteomic analysis of 14‐3‐3 binding proteins from developing barley grains

“…Using this isolation method, it was possible to obtain samples that were 70-80% intact. This is comparative to work carried out on the isolation of pea endosperm amyloplasts [34], which yielded preparations with intactness .70%. These purified preparations were run on 10% SDS-PAGE gels and electro-blotted to an NC membrane which was then incubated with anti-spinach 14-3-3 antiserum.…”

Jasmonoyl-l-Isoleucine Coordinates Metabolic Networks Required for Anthesis and Floral Attractant Emission in Wild Tobacco (Nicotiana attenuata)

“…Calculations were done according to Hill and Malmberg (1991), who described corolla RGR as the slope of ln(10 3 corolla length) over time. The RGR is constant when absolute growth rate is exponential; for the duration of the exponential phase, we used the following equation: RGR DAPi 2 j = ln (length j /length i )/(DAPi 2 DAPi).…”

“…During flower maturation, the speed and rate at which floral organs elongate vary strongly with the flower's developmental stage (Smyth et al, 1990;Hill and Malmberg, 1991). Recent studies have shown that the developmental progression of distinct floral organs after organogenesis is controlled by the interaction of differing hormonal networks that vary in their dynamics (Chandler, 2010).…”

Puzzle Box, a Tobacco Line with Flowers that Mix Floral and Inflorescence Characteristics

“…The structure of the puzzle box vegetative apex appears similar to wild type (Poethig and Sussex , 1985) , although the size is more variable. Hill and Malmberg (1991) reported that wild type tobacco produces 21 to 24 leaves prior to flowering under greenhouse conditions and that the leaf plastochron for vegetative shoots gradually decreases as flowering approaches. Under 24 C growth chamber conditions, wild type plants produce 20 to 23 leaves before flowering and the leaf plastochron gradually decreases as flowering approaches (Trull, Holaway, and Malmberg, 1992).…”