Rates of detection of Salmonella and Campylobacter in meats in response to the sample size and the infection level of each species

Tokumaru, Masakazu; Konuma, Hirotaka; Umesako, Makoto; Konno, Sumio; Shinagawa, Kunihiro

doi:10.1016/0168-1605(91)90134-b

Cited by 31 publications

(17 citation statements)

References 8 publications

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“…The frequency of contamination of red meats with Campylobacter spp. is customarily very low, and similar prevalence rates were obtained in the United States (31,93), Japan (87), and Italy (92).…”

Section: Discussionmentioning

confidence: 58%

Pet Dogs and Chicken Meat as Reservoirs ofCampylobacterspp. in Barbados

2005

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Campylobacter spp. are the second most common pathogen isolated from stools of patients with gastroenteritis in Barbados. The aim of this study was to identify reservoirs of Campylobacter and the likely source(s) of human infection. Fecal specimens from 596 animals and 311 samples of animal food products were analyzed for the presence of Campylobacter spp. by standard culture techniques. Isolates were characterized by conventional phenotypic tests, confirmed by latex agglutination and PCR with genus-specific primers, and identified by the use of species-specific primers. High isolation rates were obtained for chickens (94.2%), pigs (90.5%), dogs (46.9%), cats (37.3%), and wild birds (39.3%). Campylobacter was also recovered from monkeys (17.1%) and sheep (4.2%) but not from cows. Chicken meat was frequently contaminated with Campylobacter (58.4%), but its recovery from other animal food products was rare. Campylobacter jejuni was the most commonly identified species in humans (63.6%), chickens (86.6%), dogs (51.5%), and chicken meat (79.8%). Porcine isolates were predominantly C. coli (98.4%), while cats harbored mainly C. upsaliensis and C. helveticus. Wild birds alone carried urease-positive thermophilic campylobacters. C. jejuni and C. coli isolates from different sources were compared with isolates from humans by randomly amplified polymorphic DNA typing with the primers OPA 11 and HLWL 85. Genotyping revealed similarities between isolates from chicken meat and those from humans and could not distinguish between two clinical isolates and four canine strains. Our results suggest that dogs are significant reservoirs of Campylobacter and contribute to human enteric infections and that chicken meat is a likely vehicle for the transmission of campylobacters to humans.

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Section: Discussionmentioning

confidence: 58%

Pet Dogs and Chicken Meat as Reservoirs ofCampylobacterspp. in Barbados

2005

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“…In Japan, previous studies showed that the contamination rate was higher in chicken meat samples than pork and beef samples [11,28]. Recently, Carraminana et al [7] reported that cross contamination of Salmonella in a Spanish poultry slaughterhouse elevated the contamination rate of air-chilled carcasses to 60% during the processing.…”

Section: Discussionmentioning

confidence: 99%

“…Broilers are widely accepted as an important reservoir for human salmonellosis. Although reports on the contamination of the broiler chicken meat in slaughterhouses and retail shops have been published all over the world [3,4,7,[11][12][13][14]28], little is known about the incidence of Salmonella in broiler chickens in Japan. This study was designed to investigate the incidence of Salmonella in broiler farms to assess the status of infection with Salmonella in flocks of broiler chickens.…”

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confidence: 99%

Prevalence and Persistence of Salmonella in Broiler Chicken Flocks.

et al. 1999

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ABSTRACT. Cecal contents of 2,345 broiler chickens consisting of 28 flocks originated from 12 farms were examined for the prevalence of Salmonella to know the actual status of infection with Salmonella in the chicken flocks. Salmonella was isolated from 336 (14.3%) samples. From these isolates, eight serovars were identified. Of the 336 Salmonella isolates, 242 (72.0%) were serotyped as S. Blockley, 60 (17.9%) S. Hadar, 15 (4.5%) S. Bredeney, nine (2.7%) S. Schwarzengrund, four (1.2%) S. Anatum, three (0.9%) S. Enteritidis, two (0.6%) S. Ohio, and one (0.3%) S. Livingstone. The same serovars of Salmonella were repeatedly found in the chickens from the same farms. S. Typhimurium and S. Enteritidis were detected in pooled broken eggshell samples collected from the hatchery. Analysis of plasmid profiles revealed 11 patterns of S. Blockley and seven patterns of S. Hadar. Strains of the same plasmid profiles of S. Blockley were isolated repeatedly from the same farm over one year after the first isolation.-KEY WORDS: broiler, hatchery, poultry farm, prevalence, Salmonella.J. Vet. Med. Sci. 61(3): 255-259, 1999 cooled in an icebox and immediately transported to our laboratory. Isolation and identification of Salmonella : To each bag of cecal contents, added was 9 volumes of selenite cystine broth (SCB, Nissui). These samples were incubated at 37°C for 48 hr, then each sample was inoculated onto a brilliant green agar (BBL) plate supplemented with 20 µg novobiocin/ml (BGN) and a desoxycholate hydrogen sulfide lactose (DHL) agar (Nissui) plate. The plates were incubated at 37°C for 24 hr.

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“…Infections with S. enterica are some of the most important sources of human gastroenteritis (39). In Germany, 52,563 human salmonellosis cases were reported in 2006 (http://www3 .rki.de/SurvStat).…”

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confidence: 99%

Influence of a Probiotic Strain ofEnterococcus faeciumonSalmonella entericaSerovar Typhimurium DT104 Infection in a Porcine Animal Infection Model

Szabó

Wieler

Tedin

et al. 2009

Appl Environ Microbiol

103

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The beneficial effects of probiotic Enterococcus spp. in different hosts, such as mice and humans, have previously been reported in several studies. However, studies of large domestic animals, as well as challenge studies with pathogenic microorganisms, are very rare. Here, we investigated the influence of oral treatment of pigs with the probiotic bacterium Enterococcus faecium NCIMB 10415 on Salmonella enterica serovar Typhimurium DT104 infections in weaning piglets. Clinical symptoms, fecal excretion, the organ distribution of Salmonella, and the humoral immune response (immunoglobulin G [IgG], IgM, and IgA levels) in serum were examined. A pool of 89 piglets was randomly divided into probiotic and control groups. The probiotic group received a feed supplement containing E. faecium starting on day 14 postpartum prior to challenge with Salmonella serovar Typhimurium DT104 at 28 days postpartum. After challenge with Salmonella serovar Typhimurium DT104, piglets in both groups showed no severe clinical signs of salmonellosis. However, fecal excretion and colonization of Salmonella in organs were significantly greater in piglets fed E. faecium. Likewise, the humoral immune response against Salmonella (serum IgM and IgA levels) was significantly greater in the probiotic group animals than in control animals. The results of this study suggest that E. faecium NCIMB 10415 treatment enhanced the course of infection in weaning piglets challenged with Salmonella serovar Typhimurium DT104. However, the probiotic treatment also appeared to result in greater production of specific antibodies against Salmonella serovar Typhimurium DT104.

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Rates of detection of Salmonella and Campylobacter in meats in response to the sample size and the infection level of each species

Cited by 31 publications

References 8 publications

Pet Dogs and Chicken Meat as Reservoirs ofCampylobacterspp. in Barbados

Pet Dogs and Chicken Meat as Reservoirs ofCampylobacterspp. in Barbados

Prevalence and Persistence of Salmonella in Broiler Chicken Flocks.

Influence of a Probiotic Strain ofEnterococcus faeciumonSalmonella entericaSerovar Typhimurium DT104 Infection in a Porcine Animal Infection Model

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