Ratio of Somaclonal Variation and the Phytohormonal Content of Citrus × latifolia in Three In Vitro Culture Systems

Bulbarela-Marini, Javier Emanuel; Gómez-Meriño, Fernando Carlos; Galindo-Tovar, María Elena; Pastelín-Solano, Miriam Cristina; Murguía-González, Joaquín; Núñez-Pastrana, Rosalía; Castañeda-Castro, Odón

doi:10.1007/s00344-022-10796-x

Cited by 6 publications

(2 citation statements)

References 28 publications

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“…3 and 4). However, genetic alterations in tissue-cultured plant materials reported in some studies were rare and referred to personal error rather than the tissue culture technique itself and described to be epigenetic as the original DNA was not altered (Smulders and Klerk, 2011;Biswas et al, 2023).The tissue culture of plants could be affected by somaclonal variation and other factors like the type of plant hormones used, the time of in vitro propagation, direct-versus indirect regeneration, etc (Biswas et al, 2023;Bulbarela et al, 2023). However, most reports found a low percentage of soma clonal variation occurrences in tissue-cultured plants (Taji et al, 2002;Duta-Cornescu et al, 2023).…”

Section: Effect Of In Vitro Propagation On Genetic Stability Of S Nig...mentioning

confidence: 99%

Untitled

2023

JJBS

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Study areas include cell biology, genomics, microbiology, immunology, molecular biology, biochemistry, embryology, immunogenetics, cell and tissue culture, molecular ecology, genetic engineering and biological engineering, bioremediation and biodegradation, bioinformatics, biotechnology regulations, gene therapy, organismal biology, microbial and environmental biotechnology, marine sciences. The JJBS welcomes the submission of manuscript that meets the general criteria of significance and academic excellence. All articles published in JJBS are peerreviewed. Papers will be published approximately one to two months after acceptance.

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Section: Effect Of In Vitro Propagation On Genetic Stability Of S Nig...mentioning

confidence: 99%

Untitled

2023

JJBS

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“…In the plant tissue culture studies, the genetic homogeneity of the in vitro propagated plants in most cases is still constant. The soma-clonal variation in the plant tissue culture is affected by the growth regulators used and the direct and indirect regeneration [61,62]. However, most reports found a low percentage of somaclonal variation occurrence in tissue-cultured plants [63,64].…”

Section: Cryopreservation Of S Dominica Callusmentioning

confidence: 99%

A Sustainable Approach to In Vitro Propagation and Conservation of Salvia dominica L.: A Wild Medicinal Plant from Jordan

Al-Qudah,

Shibli,

Zatimeh

et al. 2023

Sustainability

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Salvia dominica L. is an important wild medicinal plant that grows in Jordan and neighboring countries, and this plant has been suffering from many threats in its wild environment. Therefore, this research aims to establish a comprehensive and sustainable approach via an in vitro propagation and conservation system for the S. dominica L. plant. Axillary buds were used to initiate the in vitro culture on Murashige and Skoog MS media supplemented with 0.5 mg L−1 of GA3. In vitro shoot proliferation and rooting were experimented on with different concentrations of cytokinins and auxins, respectively. Calli were induced in the dark on excised leaf discs (0.5 cm in diameter), and multiplication was experimented on with different growth regulators. Cryopreservation experiments were applied on the callused segments under different growth conditions via the vitrification technique. A full protocol was achieved for shoot proliferation with 6.3 shoots/explant using 1.2 mg L−1 of thidiazuron (TDZ), while rooting was achieved at 1.5 mg L−1 of NAA with 6.6 functional roots/explant. Acclimatization was completely successful for the rooted plants. The highest callus production with 5.81 g/calli was achieved using 1.5 mg L−1 of benzylaminopurine (BAP). Cryopreservation of the S. dominica calli was successfully achieved when a pure plant vitrification solution (PVS2) was used to dehydrate the calli for 20 min after immersion in the loading solution for 20 min with a 76.6% regrowth percentage. The loading and the plant vitrification solution type and duration were the most critical points in the regrowth of the cryopreserved calli. In conclusion, a successful protocol was set up for the in vitro propagation and conservation of S. dominica calli. This study has prompted us to perform further studies on sustainable in vitro production and conservation of critically endangered medicinal plants to implement a green environment protecting against surrounding threats.

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Factors Affecting Tissue Culture-Induced Variations

Sánchez-Romero

2024

Somaclonal Variation: Basic and Practical Aspects

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Ratio of Somaclonal Variation and the Phytohormonal Content of Citrus × latifolia in Three In Vitro Culture Systems

Cited by 6 publications

References 28 publications

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A Sustainable Approach to In Vitro Propagation and Conservation of Salvia dominica L.: A Wild Medicinal Plant from Jordan

Factors Affecting Tissue Culture-Induced Variations

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