Ratio Spectra Derivative and Zero-Crossing Difference Spectrophotometric Determination of Olmesartan Medoxomil and Hydrochlorothiazide in Combined Pharmaceutical Dosage Form

Abstract

Abstract. Two simple, economical, rapid, precise, and accurate methods for simultaneous determination of olmesartan medoxomil and hydrochlorothiazide in combined tablet dosage form have been developed. The first method is based on ratio spectra derivative spectrophotometry, and the second method is zerocrossing difference spectrophotometry. The amplitudes in the first derivative of the corresponding ratio spectra at 231.0 and 271.0 nm were selected to determine olmesartan medoxomil and hydrochlorothiazide, respectively. Measurements of absorbance were carried out at zero-crossing wavelengths 257.8 and 240.2 nm for olmesartan medoxomil and hydrochlorothiazide by zero-crossing difference spectrophotometric method. Beer's law is obeyed in the concentration range of 08-24 µg/mL for olmesartan medoxomil (OLM) and 05-15 µg/mL for hydrochlorothiazide (HCT) by ratio spectra derivative and 05-30 µg/mL for OLM and HCT by zero-crossing difference spectrophotometric method. The results of the assay were found to be 100.46±0.95 for OLM and 100.4±0.27 for HCT by ratio spectra derivative and 99.06±1.14 for OLM and 100.05±0.90 for HCT by zero-crossing difference spectrophotometric method. These methods passes F test and t test. Both methods were validated statistically and by performing recovery study.

Simultaneous determination and validation of some binary mixtures of antihypertensive drugs using ratio derivative spectrophotometric method

“…OLM and HCT binary mixtures have already been studied using DD 1 method [5] in 0.1 M NaOH solu tions, while ZOF-HCT mixture has not yet analyzed with that method. Also, OLM-HCT and ZOF-HCT mixtures are not yet officially found in any pharmaco poeia.…”

“…Several methods have been reported concerning si multaneous determination of OLM and HCT [4][5][6][7][8][9][10][11][12][13] in pharmaceutical dosage form or biological samples using UV spectrophotometric [4][5][6], LC-MS/MS [7], TLC and HPTLC [8][9][10], and LC [10][11][12][13]. Even though some procedures have been described for the assay of either ZOF or HCT in dosage forms, there are only limited methods such as LC [14,15] and deriva tive spectrophotometry [16] able to determine ZOF and HCT simultaneously [16].…”

A comparative study of two separate analytical techniques for the simultaneous determination of diclofenac sodium and diacerein from combined dosage form

“…A study of variables like concentration of the standard spectrum divisor, number of points for the smoothing function and Δλ were necessary for the simultaneous determination of DS and DC using first derivative ratio spectroscopy in order to optimize the signal of the ratio derivative spectra with good selectivity and higher sensitivity (Rote, Bari, 2009). Concentrations of 5µg /mL of DC (DC5) and 6 µg /ml of DS (DS6) were selected as divisor concentration.…”

Simultaneous Determination of Ambroxol, Guaiphenesin and Levosalbutamol Sulphate in Pharmaceutical Formulations With the Use of Four Rapid Derivative Spectrophotometric Methods

“…In this work, various orders of derivative and different kinds of measurements were assayed, i.e., zero-crossing first derivative [8][9][10], simultaneous equation method [11][12], ratio-spectra first derivative zero crossing [13] and double divisor ratio spectra derivative method [14]. Four methods have been successfully developed for mentioned combination and satisfactory results were obtained.…”