Ratiometric Barcoding for Mass Cytometry

Wu, Xu; DeGottardi, Quinn; Wu, I‐Chin; Li, Wu; Yu, Jian; Kwok, William W.; Chiu, Daniel T.

doi:10.1021/acs.analchem.8b03201

Cited by 10 publications

(10 citation statements)

References 33 publications

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“…Given that our barcoding nanosystem does not interfere with cellular proteins, fulfilling the main criteria for a universal cell barcoding, we believe that it could be compatible with other live-cell barcoding reagents, such as TeMal, 36 platinum-labeled antibodies targeting b2m and CD298, 33 and ratiometric barcoding with Pdots. 58 …”

Section: Resultsmentioning

confidence: 99%

Hybrid Fluorescent Mass-Tag Nanotrackers as Universal Reagents for Long-Term Live-Cell Barcoding

et al. 2022

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Barcoding and pooling cells for processing as a composite sample are critical to minimize technical variability in multiplex technologies. Fluorescent cell barcoding has been established as a standard method for multiplexing in flow cytometry analysis. In parallel, mass-tag barcoding is routinely used to label cells for mass cytometry. Barcode reagents currently used label intracellular proteins in fixed and permeabilized cells and, therefore, are not suitable for studies with live cells in long-term culture prior to analysis. In this study, we report the development of fluorescent palladium-based hybrid-tag nanotrackers to barcode live cells for flow and mass cytometry dual-modal readout. We describe the preparation, physicochemical characterization, efficiency of cell internalization, and durability of these nanotrackers in live cells cultured over time. In addition, we demonstrate their compatibility with standardized cytometry reagents and protocols. Finally, we validated these nanotrackers for drug response assays during a long-term coculture experiment with two barcoded cell lines. This method represents a new and widely applicable advance for fluorescent and mass-tag barcoding that is independent of protein expression levels and can be used to label cells before long-term drug studies.

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Section: Resultsmentioning

confidence: 99%

Hybrid Fluorescent Mass-Tag Nanotrackers as Universal Reagents for Long-Term Live-Cell Barcoding

et al. 2022

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“…reported a method to concurrently screen antigen‐specific T cells epitopes in a single sample by preparing 120 ratiometric codes using 10 metal isotopes . Chiu's group developed a ratiometric barcoding strategy by employing three metal isotopes for mass cytometric analysis. Zhang's group designed an accurate and multiplex assay to perform the isotope dilution for the absolute quantitative analysis of metal isotope‐tagged DNA targets.…”

Section: Methodsmentioning

confidence: 99%

Ratiometric DNA Walking Machine for Accurate and Amplified Bioassay

Wang

Liu

et al. 2019

Chemistry A European J

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DNA walkingm achines openedn ew avenues for the biosensing and demonstrated great successi nthe past few years.S inceD NA machines are mainly nonequilibrium systemsd riven by dynamic interactions, the matrix effects on DNA machines is ab ottlenecka nd more intricate than common DNA-mediated assays,e speciallyf or complicated physiological samples. Herein, to realizea n accurate and reliable quantitative machine, ar atiometric DNA walking machinew as developedi nh uman serums and cell lysatesb ased on the elemental isotoper atio measurement. The target DNA-triggered walking machine converted and amplified biological signals into mass spectrometric signal ratios ( 197 Au/ 115 In) via ab urnt-bridge mechanism. Under the optimized conditions, the limit of detection (LOD, 3s)w as 8f Mf or target DNA,w ith ad ynamic linear range of 0.05-0.7 pM. The ratiometric DNA walking machine was directly appliedi nh uman serum samples with satisfactory recoveries of 94 to 105 %, demonstrating an excellent stability and ah igh accuracy.C ombining the aptamer-based specific recognition,t he proposedD NA machine is expected to be av ersatile platform for other targets,s uch as small biomolecules and proteins.Supporting information and the ORCID identification number(s) for the author(s) of this article can be found under: https://doi.

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“…Narrower Pdot size distributions are anticipated to enhance the performance further . Pdot-based mass cytometry barcodes were subsequently generated by varying the identities and amounts of multiple metal isotopes in the Pdots, allowing for the coding of 16 sets of labeled cells …”

Section: Chemical Analysis and Bioanalysismentioning

confidence: 99%

Photoluminescent Nanoparticles for Chemical and Biological Analysis and Imaging

et al. 2021

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Research related to the development and application of luminescent nanoparticles (LNPs) for chemical and biological analysis and imaging is flourishing. Novel materials and new applications continue to be reported after two decades of research. This review provides a comprehensive and heuristic overview of this field. It is targeted to both newcomers and experts who are interested in a critical assessment of LNP materials, their properties, strengths and weaknesses, and prospective applications. Numerous LNP materials are cataloged by fundamental descriptions of their chemical identities and physical morphology, quantitative photoluminescence (PL) properties, PL mechanisms, and surface chemistry. These materials include various semiconductor quantum dots, carbon nanotubes, graphene derivatives, carbon dots, nanodiamonds, luminescent metal nanoclusters, lanthanidedoped upconversion nanoparticles and downshifting nanoparticles, triplet−triplet annihilation nanoparticles, persistent-luminescence nanoparticles, conjugated polymer nanoparticles and semiconducting polymer dots, multi-nanoparticle assemblies, and doped and labeled nanoparticles, including but not limited to those based on polymers and silica. As an exercise in the critical assessment of LNP properties, these materials are ranked by several application-related functional criteria. Additional sections highlight recent examples of advances in chemical and biological analysis, point-of-care diagnostics, and cellular, tissue, and in vivo imaging and theranostics. These examples are drawn from the recent literature and organized by both LNP material and the particular properties that are leveraged to an advantage. Finally, a perspective on what comes next for the field is offered.

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Ratiometric Barcoding for Mass Cytometry

Cited by 10 publications

References 33 publications

Hybrid Fluorescent Mass-Tag Nanotrackers as Universal Reagents for Long-Term Live-Cell Barcoding

Hybrid Fluorescent Mass-Tag Nanotrackers as Universal Reagents for Long-Term Live-Cell Barcoding

Ratiometric DNA Walking Machine for Accurate and Amplified Bioassay

Photoluminescent Nanoparticles for Chemical and Biological Analysis and Imaging

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