Ratiometric luminescence 2D<i>in vivo</i>imaging and monitoring of mouse skin oxygenation

Hofmann, Julian; Meier, Robert J.; Mahnke, Alexander; Schatz, Valentin; Brackmann, Florian; Trollmann, Regina; Bogdan, Christian; Liebsch, Gregor; Wang, Xudong; Wolfbeis, Otto S.

doi:10.1088/2050-6120/1/4/045002

Cited by 34 publications

(26 citation statements)

References 52 publications

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“…S1A, Experimental Procedures). The sensor consisted of a red luminescent O2-dependent probe and a green luminescent reference dye, both immobilized in a highly O2-permeable polymer matrix to form a sensor layer that was fixed on a transparent and O2-blocking polyester support (Hofmann et al, 2013). This guarantees that the sensor detects only O2 on the side of the sensor layer that was facing towards the mouse cecal tissue.…”

Section: Transserosal Luminescence Two-dimensional In Vivo Tissue Oxymentioning

confidence: 99%

“…S1A). The RLI sensor system was used to monitor O2 levels as described earlier (Hofmann et al, 2013). In brief, the measurement set-up consisted of a transparent O2-sensitive foil (prototype O2 sensor set SF-RPSu4-NAU-L4/OIW, Presens, Regensburg, Germany) and a portable USB-connected RLI device (USB microscope VisiSens A1 prototype, Presens) that excites the sensor foils with light-emitting diodes (blue light) and records with RGB chip the red and green light emitted from the sensor foil.…”

Section: Transserosal Oxygen Sensing Of Cecal Tissue Using Ratiometrimentioning

confidence: 99%

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Low-oxygen tensions found inSalmonella-infected gut tissue boostSalmonellareplication in macrophages by impairing antimicrobial activity and augmentingSalmonellavirulence

Jennewein

Matuszak

Walter

et al. 2015

Cellular Microbiology

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In Salmonella infection, the Salmonella pathogenicity island-2 (SPI-2)-encoded type three secretion system (T3SS2) is of key importance for systemic disease and survival in host cells. For instance, in the streptomycin-pretreated mouse model SPI-2-dependent Salmonella replication in lamina propria CD11c(-)CXCR1(-) monocytic phagocytes/macrophages (MΦ) is required for the development of colitis. In addition, containment of intracellular Salmonella in the gut critically depends on the antimicrobial effects of the phagocyte NADPH oxidase (PHOX), and possibly type 2 nitric oxide synthase (NOS2). For both antimicrobial enzyme complexes, oxygen is an essential substrate. However, the amount of available oxygen upon enteroinvasive Salmonella infection in the gut tissue and its impact on Salmonella-MΦ interactions was unknown. Therefore, we measured the gut tissue oxygen levels in a model of Salmonella enterocolitis using luminescence two-dimensional in vivo oxygen imaging. We found that gut tissue oxygen levels dropped from ∼78 Torr (∼11% O2) to values of ∼16 Torr (∼2% O2) during infection. Because in vivo virulence of Salmonella depends on the Salmonella survival in MΦ, Salmonella-MΦ interaction was analysed under such low oxygen values. These experiments revealed an increased intracellular replication and survival of wild-type and t3ss2 non-expressing Salmonella. These findings were paralleled by blunted nitric oxide and reactive oxygen species (ROS) production and reduced Salmonella ROS perception. In addition, hypoxia enhanced SPI-2 transcription and translocation of SPI-2-encoded virulence protein. Neither pharmacological blockade of PHOX and NOS2 nor impairment of T3SS2 virulence function alone mimicked the effect of hypoxia on Salmonella replication under normoxic conditions. However, if t3ss2 non-expressing Salmonella were used, hypoxia did not further enhance Salmonella recovery in a PHOX and NOS2-deficient situation. Hence, these data suggest that hypoxia-induced impairment of antimicrobial activity and Salmonella virulence cooperate to allow for enhanced Salmonella replication in MΦ.

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Section: Transserosal Luminescence Two-dimensional In Vivo Tissue Oxymentioning

confidence: 99%

Section: Transserosal Oxygen Sensing Of Cecal Tissue Using Ratiometrimentioning

confidence: 99%

Low-oxygen tensions found inSalmonella-infected gut tissue boostSalmonellareplication in macrophages by impairing antimicrobial activity and augmentingSalmonellavirulence

Jennewein

Matuszak

Walter

et al. 2015

Cellular Microbiology

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“…RLI-sensor foils were two-point calibrated using Na 2 S 2 O 5 and air-saturated distilled water before use. 54 Pimonidazole analyses were performed by administration of pimonidazole (1 mM hypoxyprobe-1; Chemicon International, Temecula, CA) to the plMDCK cysts within the matrix overnight before fixation with paraformaldehyde (4%). Whole collagen blobs then were incubated with hypoxyprobe MAb1 (1:50) conjugated with FITC.…”

Section: Oxygen Measurementsmentioning

confidence: 99%

Hypoxia-Inducible Factor-1α Causes Renal Cyst Expansion through Calcium-Activated Chloride Secretion

Buchholz

Schley

Faria

et al. 2014

Journal of the American Society of Nephrology

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Polycystic kidney diseases are characterized by numerous bilateral renal cysts that continuously enlarge and, through compression of intact nephrons, lead to a decline in kidney function over time. We previously showed that cyst enlargement is accompanied by regional hypoxia, which results in the stabilization of hypoxia-inducible transcription factor-1a (HIF-1a) in the cyst epithelium. Here we demonstrate a correlation between cyst size and the expression of the HIF-1a-target gene, glucose transporter 1, and report that HIF-1a promotes renal cyst growth in two in vitro cyst models-principal-like MDCK cells (plMDCKs) within a collagen matrix and cultured embryonic mouse kidneys stimulated with forskolin. In both models, augmenting HIF-1a levels with the prolyl hydroxylase inhibitor 2-(1-chloro-4-hydroxyisoquinoline-3-carboxamido) acetate enhanced cyst growth. In addition, inhibition of HIF-1a degradation through tubule-specific knockdown of the von Hippel-Lindau tumor suppressor increased cyst size in the embryonic kidney cyst model. In contrast, inhibition of HIF-1a by chetomin and knockdown of HIF-1a both decreased cyst growth in these models. Consistent with previous reports, plMDCK cyst enlargement was driven largely by transepithelial chloride secretion, which consists, in part, of a calcium-activated chloride conductance. plMDCKs deficient for HIF-1a almost completely lacked calcium-activated chloride secretion. We conclude that regional hypoxia in renal cysts contributes to cyst growth, primarily due to HIF-1a-dependent calcium-activated chloride secretion. These findings identify the HIF system as a novel target for inhibition of cyst growth.

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“…Such an approach would not, in principle, require any patient-specific calibration and could be carried out repeatedly under physiological conditions. Pioneering work in the development of such sensing films has been reported by Wolfbeis and his colleagues [23], including their use in visualizing wounds [24] and infections [25] via both lifetime measurements and measuring changes in phosphorescence intensity [26].…”

Section: Introductionmentioning

confidence: 99%

Non-invasive transdermal two-dimensional mapping of cutaneous oxygenation with a rapid-drying liquid bandage

Roussakis

Koolen

et al. 2014

Biomed. Opt. Express

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Oxygen plays an important role in wound healing, as it is essential to biological functions such as cell proliferation, immune responses and collagen synthesis. Poor oxygenation is directly associated with the development of chronic ischemic wounds, which affect more than 6 million people each year in the United States alone at an estimated cost of $25 billion. Knowledge of oxygenation status is also important in the management of burns and skin grafts, as well as in a wide range of skin conditions. Despite the importance of the clinical determination of tissue oxygenation, there is a lack of rapid, user-friendly and quantitative diagnostic tools that allow for non-disruptive, continuous monitoring of oxygen content across large areas of skin and wounds to guide care and therapeutic decisions. In this work, we describe a sensitive, colorimetric, oxygen-sensing paint-on bandage for two-dimensional mapping of tissue oxygenation in skin, burns, and skin grafts. By embedding both an oxygen-sensing porphyrin-dendrimer phosphor and a reference dye in a liquid bandage matrix, we have created a liquid bandage that can be painted onto the skin surface and dries into a thin film that adheres tightly to the skin or wound topology. When captured by a camera-based imaging device, the oxygen-dependent phosphorescence emission of the bandage can be used to quantify and map both the pO2 and oxygen consumption of the underlying tissue. In this proof-of-principle study, we first demonstrate our system on a rat ischemic limb model to show its capabilities in sensing tissue ischemia. It is then tested on both ex vivo and in vivo porcine burn models to monitor the progression of burn injuries. Lastly, the bandage is applied to an in vivo porcine graft model for monitoring the integration of full- and partial-thickness skin grafts.

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Ratiometric luminescence 2Din vivoimaging and monitoring of mouse skin oxygenation

Cited by 34 publications

References 52 publications

Low-oxygen tensions found inSalmonella-infected gut tissue boostSalmonellareplication in macrophages by impairing antimicrobial activity and augmentingSalmonellavirulence

Low-oxygen tensions found inSalmonella-infected gut tissue boostSalmonellareplication in macrophages by impairing antimicrobial activity and augmentingSalmonellavirulence

Hypoxia-Inducible Factor-1α Causes Renal Cyst Expansion through Calcium-Activated Chloride Secretion

Non-invasive transdermal two-dimensional mapping of cutaneous oxygenation with a rapid-drying liquid bandage

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