Ratiometric photoacoustic imaging of endogenous HNOin vivofor assessing prodrug release and liver injury

Abstract

The effective imaging of endogenous HNO is great crucial for pathology research and medical development due to its important pharmacology activity in biological systems. Here, a ratiometric photoacoustic probe in...

An activatable fluorescent-photoacoustic dual-modal probe for highly sensitive imaging of nitroxyl in vivo

“…In the presence of HNO, specific cleavage of the triarylphosphine group of the probe leads to the release of the hydroxyl group, resulting in the activation of intense NIRF/PA signals during the recovery of the ICT process. Compared with reported NIRF 19–21,30,31 and NIRF/PA probes, 28,29 QL-HNO is confirmed to exhibit the highest sensitivity in NIRF mode and a desirable PA signal-to-noise ratio for HNO detection in vitro (Table S1†). QL-HNO has also been successfully utilized for visualizing endogenous and exogenous HNO both in living cells and tumors.…”

“…22 In comparison, as a novel biomedical imaging technique which can convert light excitation into ultrasonic signals, 23–25 photoacoustic (PA) imaging technology principally diminishes the effects of light scattering and allows deeper tissue penetration and higher three-dimensional spatial resolution imaging of live animals. 26,27 A PA imaging probe 28 and a NIRF/PA dual-modal imaging probe 29 based on hemicyanine were designed for HNO imaging in vivo . However, the sensitivity and PA signal-to-noise ratio are still unsatisfactory, which may limit their further application in the diagnosis of HNO-associated diseases.…”

Advances in Carbon Dot-Based Ratiometric Fluorescent Probes for Environmental Contaminant Detection: A Review

“…Ratiometric fluorescent probes play a crucial role in environmental monitoring, offering precise and reliable detection of various contaminants. Several ratiometric technologies are available, including Fluorescence Resonance Energy Transfer (FRET) [ 14 ], Chemiluminescence (CL) [ 15 ], Photoacoustic (PA) [ 16 ], Bioluminescence (BL) [ 17 ], and Afterglow [ 18 ], each with its own set of advantages and limitations. For example, FRET provides high sensitivity and resolution but requires matching fluorescent moieties and operates within limited distance ranges.…”