Ratiometric sensing of α-glucosidase and its inhibitor based on MnO2 nanosheets promoted in-situ fluorescent reactions

Cerium-Based Metal–Organic Framework Nanocrystals for Colorimetric Screening of α-Glucosidase Inhibitors

“…The IC 50 value was determined to be 7.04 μM, which is consistent with previous works' reported values of IC 50 . 26,27 This indicates that the established system possesses good reliability for in vitro screening of α-Glu inhibitors. Furthermore, from Figure 9b, it can be observed that the inhibition rate increases with the concentration of acarbose, exhibiting a good linear relationship within the range of 1.25−15 μM.…”

The construction of dual-emissive ratiometric fluorescent probes based on fluorescent nanoparticles for the detection of metal ions and small molecules

“…Accordingly, to improve the selectivity of FNP-nanozyme-o-PD ternary systems, a nanozyme-o-PD binary system was proposed for the detection of AA, which can reduce the interference from biothiols. [336][337][338][339][340] Using MnO 2 nanosheets as a model nanozyme, the detection mechanism is presented in Fig. 17c.…”

“…336 This MnO 2 -o-PD system could be used to detect AA 336 and AArelated reactions, such as ALP. 337 Based on a similar mechanism, an MnO 2 NS-o-PD ratiometric fluorescence system was proposed for the detection of the activity of α-glucosidase (α-Glu), which can catalyze the substrate 2-O-α-D-glucopyranose-L-ascorbic acid (AAG) to produce AA, 338 and the CuO NPo-PD ratiometric fluorescence system was proposed for the detection of AA in serum and fruits. 339 The Cu 2+ -AA-o-PD ternary system was proposed for the detection of bleomycin, which complexes with Cu 2+ to form larger CuNPs, weakening the autocatalytic activity of Cu 2+ .…”

In situ green synthesis of the nanocomposites of MnO2/graphene as an oxidase mimic for sensitive colorimetric and electrochemical dual-mode biosensing

“…4b, the peaks of O 1s at 529, 530.6 and 532.5 eV can be respectively attributed to Mn-O-Mn, C]O and C-OH. 41,42 In detail, the C 1s peak can be deconvoluted into three components, with binding energies at 284, 285.3 and 287.6 eV (Fig. 4c), which are ascribed to the graphitic carbons, C-O bonds and C]O bonds.…”