Rational design of a luminescent nanoprobe for hypoxia imaging in vivo via ratiometric and photoluminescence lifetime imaging microscopy

Qi, Yu; Huang, Tianci; Li, Yipeng; Wei, Huanjie; Liu, Shujuan; Huang, Wei; Du, Jing; Zhao, Qiang

doi:10.1039/c7cc00668c

Cited by 29 publications

(20 citation statements)

References 37 publications

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“…In the first situation, no obvious interaction occurs between materials, where the self-luminous nanomaterial is only used as a reference for ratiometric detection. Currently, these frequently-used self-referenced luminous nanoparticles mainly consist of dye-embedded silica, 153, 154 gold nanoclusters (AuNCs), 155–157 fluorescent bovine serum albumin nanoparticles, 158 Cdots, 159, 160 , silicon nanodots, 161 or quantum dots (QDs). 162, 163 Through a combination of the corresponding target-sensitive fluorescent dye units with unique responses to target analytes or molecular events, these dual-emission nanoparticle-dye nanoconjugates have been extensively applied for ratiometric sensing and imaging of diverse physical and physiological changes.…”

Section: Ratiometric Fluorescence Nanoprobesmentioning

confidence: 99%

Ratiometric optical nanoprobes enable accurate molecular detection and imaging

et al. 2018

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Exploring and understanding biological and pathological changes are of great significance for early diagnosis and therapy of diseases. Optical sensing and imaging approaches have experienced major progress in this field. Particularly, an emergence of various functional optical nanoprobes has provided enhanced sensitivity, specificity, targeting ability, as well as multiplexing and multimodal capabilities due to improvements in their intrinsic physicochemical and optical properties. However, one of the biggest challenges of conventional optical nanoprobes is their absolute intensity-dependent signal readout, which causes inaccurate sensing and imaging results due to the presence of various analyte-independent factors that can cause fluctuations in their absolute signal intensity. Ratiometric measurements provide built-in self-calibration for signal correction, enabling more sensitive and reliable detection. Optimizing nanoprobe designs with ratiometric strategies can surmount many of the limitations encountered by traditional optical nanoprobes. This review first elaborates upon existing optical nanoprobes that exploit ratiometric measurements for improved sensing and imaging, including fluorescence, surface enhanced Raman scattering (SERS), and photoacoustic nanoprobes. Next, a thorough discussion is provided on design strategies for these nanoprobes, and their potential biomedical applications for targeting specific biomolecule populations (e.g. cancer biomarkers and small molecules with physiological relevance), for imaging the tumor microenvironment (e.g. pH, reactive oxygen species, hypoxia, enzyme and metal ions), as well as for intraoperative image guidance of tumor-resection procedures.

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Section: Ratiometric Fluorescence Nanoprobesmentioning

confidence: 99%

Ratiometric optical nanoprobes enable accurate molecular detection and imaging

et al. 2018

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“…Thus, such coupling reaction has little influence on fluorescence of nanomaterials and is widely used to prepare fluorescent nanocomposites. Indeed, some NCs‐based ratiometric fluorescent probes have been prepared by carbodiimide‐activated coupling reaction due to the fact that the surface of NCs usually contains a large amount of carboxyl groups or amino groups . For example, carboxyl‐functionalized Au NCs and amino‐functionalized carbon dots (CDs) can be directly bonded together to afford the assembled C‐dots‐AuNCs binary heterogeneous nanocomplexes via carbodiimide‐activated coupling reaction ( Figure a) .…”

Section: Sensor Designmentioning

confidence: 99%

“…Resultant DEFNs display ideal single‐excitation, dual‐emission with two well‐resolved, intensity‐comparable fluorescence peaks, and function in optical thermometry with high reliability and accuracy by exploiting the temperature sensitivity of their fluorescence intensity ratio (blue/red). Yu et al reported a dual‐emitting fluorescent probe by using EDC/NHS as an active agent to carry out carbodiimide‐activated coupling of Au NCs with amino‐containing iridium complexes …”

Section: Sensor Designmentioning

confidence: 99%

Metal Nanoclusters–Based Ratiometric Fluorescent Probes from Design to Sensing Applications

Zhao

Wang

et al. 2019

Part & Part Syst Charact

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The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/ppsc.201900298. Ratiometric fluorescent probes have many advantages including improved sensitivity, high reliability and accuracy, visualization sensing, etc. Herein, the development of metal nanoclusters (NCs)-based ratiometric fluorescent probes in the recent years is summarized. NCs, an emerging new class of fluorescent nanomaterials, have demonstrated excellent optical properties, good biocompatibility, great aqueous solubility, low cost, and simple synthesis, and NCs-based ratiometric fluorescent probes have attracted much attention. In this progress report, the preparation and properties of NCs and the design of ratiometric fluorescent probes are summarized. Sensing of a broad range of analytes including cations, gas, small molecules, macromolecules, temperature, and pH is discussed. In addition, the challenges and future directions for NCs-based ratiometric fluorescent probes are also presented.

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“…However, while TRAST is based on the readout of a strong fluorescence signal, the RTP signal is weak and susceptible to dynamic quenching by oxygen and trace impurities. Moreover, while TRAST can be based on a broad range of different fluorophores and even auto-fluorescent compounds, specific RTP probes are scarce and cannot easily be loaded into cells (Yu et al 2017 ). In contrast to RTP, TRAST is also not limited to imaging of triplet state parameters only.…”

Section: Transient-state (Trast) Spectroscopy/imaging To Exploit the mentioning

confidence: 99%

Fluorescence-based monitoring of electronic state and ion exchange kinetics with FCS and related techniques: from T-jump measurements to fluorescence fluctuations

Rigler

Widengren

2017

Eur Biophys J

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In this review, we give a historical view of how our research in the development and use of fluorescence correlation spectroscopy (FCS) and related techniques has its roots and how it originally evolved from the pioneering work of Manfred Eigen, his colleagues, and coworkers. Work on temperature-jump (T-jump) experiments, conducted almost 50 years ago, led on to the development of the FCS technique. The pioneering work in the 1970s, introducing and demonstrating the concept for FCS, in turn formed the basis for the breakthrough use of FCS more than 15 years later. FCS can be used for monitoring reaction kinetics, based on fluctuations at thermodynamic equilibrium, rather than on relaxation measurements following perturbations. In this review, we more specifically discuss FCS measurements on photodynamic, electronic state transitions in fluorophore molecules, and on proton exchange dynamics in solution and on biomembranes. In the latter case, FCS measurements have proven capable of casting new light on the mechanisms of proton exchange at biological membranes, of central importance to bioenergetics and signal transduction. Finally, we describe the transient-state (TRAST) spectroscopy/imaging technique, sharing features with both relaxation (T-jump) and equilibrium fluctuation (FCS) techniques. TRAST is broadly applicable for cellular and molecular studies, and we briefly outline how TRAST can provide unique information from fluorophore blinking kinetics, reflecting e.g., cellular metabolism, rare molecular encounters, and molecular stoichiometries.

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Rational design of a luminescent nanoprobe for hypoxia imaging in vivo via ratiometric and photoluminescence lifetime imaging microscopy

Abstract: A luminescent nanoprobe has been designed for detection of oxygen. The nanoprobe exhibits high sensitivity, selectivity and excellent reversibility, and has been employed for hypoxia imaging in vitro and in vivo by ratiometric and photoluminescence lifetime imaging techniques.

Cited by 29 publications

References 37 publications

Ratiometric optical nanoprobes enable accurate molecular detection and imaging

Ratiometric optical nanoprobes enable accurate molecular detection and imaging

Metal Nanoclusters–Based Ratiometric Fluorescent Probes from Design to Sensing Applications

Fluorescence-based monitoring of electronic state and ion exchange kinetics with FCS and related techniques: from T-jump measurements to fluorescence fluctuations

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