Rational design to change product specificities and thermostability of cyclodextrin glycosyltransferase from Paenibacillus sp.

Abstract

Functional modification of cyclodextrin glycosyltransferase (CGTases) for better product specificity and thermostability is of great importance for industrial applications.

Rational design of cholesterol oxidase for efficient bioresolution of cholestane skeleton substrates

“…Protein engineering provides a rigorous strategy and allows the creation of novel enzymes for biotechnological applications 14 , 15 . Rational design based on structure and function relationships has achieved significant success for improving enzymatic performance 16 , 17 . For instance, Toyama and co-workers introduced amino acid substitutions into Streptomyces cholesterol oxidase using site-directed mutagenesis and structural comparisons to investigate substrate specificity and affinity 18 .…”