Rational engineering of glycosaminoglycan-based Dickkopf-1 scavengers to improve bone regeneration

Ruiz-Gómez, Gloria; Salbach-Hirsch, Juliane; Dürig, Jan-Niklas; Köhler, Linda; Balamurugan, Kanagasabai; Rother, Sandra; Heidig, Sophie-Luise; Moeller, Stephanie; Schnabelrauch, Matthias; Furesi, Giulia; Pählig, Sophie; Guillem-Gloria, Pedro M.; Hofbauer, Christine; Hintze, Vera; Pisabarro, M. Teresa; Rademann, Jörg; Hofbauer, Lorenz C.

doi:10.1016/j.biomaterials.2023.122105

Biomaterials

2023

DOI: 10.1016/j.biomaterials.2023.122105

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Rational engineering of glycosaminoglycan-based Dickkopf-1 scavengers to improve bone regeneration

Gloria Ruiz-Gómez¹,

Juliane Salbach-Hirsch²,

Jan-Niklas Dürig³

et al.

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Cited by 2 publications

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Modulating Lineage Specification in Stem Cell Differentiation via Bioelectrical Stimulation Intensity Matching

Zhang,

Yan,

et al. 2023

Adv Materials Inter

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Development and regeneration in biological tissues are fundamentally affected by stem‐cell‐fate commitment. Bioelectricity is heterogeneous between different tissues and crucially regulates cell behaviors, including cell differentiation. However, the effects of heterogeneous bioelectricity on stem‐cell differentiation remain poorly understood. Herein, it is shown that providing stem cells with electrical stimulation matching the endogenous membrane potentials of cells derived from different tissues (osteogenic‐related: −55.05 ± 4.22 mV, neurogenic‐related: −84.8 ± 7.48 mV) can induce their osteogenic or neurogenic lineage commitment. Molecular dynamics simulations indicated that the osteogenic‐related surface potential favors the adsorption of fibronectin, while the neurogenic‐related surface potential enhances the adsorption of FGF‐2. These different protein adsorptions trigger either downstream Wnt or Erk signaling, which direct stem‐cell differentiation. Surface‐potential‐mediated lineage‐specification of stem cells using bioelectrical intensity has enormous potential application value in tissue regenerative therapy.

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Modulating Lineage Specification in Stem Cell Differentiation via Bioelectrical Stimulation Intensity Matching

Zhang,

Yan,

et al. 2023

Adv Materials Inter

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Dimerization and Crowding in the Binding of Interleukin 8 to Dendritic Glycosaminoglycans as Artificial Proteoglycans

Dürig,

Schulze,

Bosse

et al. 2024

Chemistry A European J

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Interactions of glycosaminoglycans (GAG) with proteins of the extracellular matrix govern and regulate complex physiological functions including cellular growth, immune response, and inflammation. Repetitive presentation of GAG binding motifs as found in native proteoglycans might enhance GAG–protein binding through multivalent interactions. Here, we report the chemical synthesis of dendritic GAG oligomers constructed of nona‐sulfated hyaluronan tetrasaccharides for investigating the binding of the protein chemokine interleukin 8 (IL‐8) to artificial, well‐defined proteoglycan architectures. Binding of mutant monomeric and native dimerizable IL‐8 was investigated by nuclear magnetic resonance (NMR) spectroscopy and isothermal titration calorimetry. Dendritic oligomerization of GAG increased the binding affinity of both monomeric and dimeric IL‐8. Monomeric IL‑8 bound to monomeric and dimeric GAG with KD values of 7.3 µM and 0.108 µM, respectively. The effect was less pronounced for dimerizable wildtype IL‐8, for which GAG dimerization improved the affinity from 34 nM to 5 nM. Binding of dimeric IL‐8 to oligomeric GAG was limited by steric crowding effects, strongly reducing the affinity of subsequent binding events. In conclusion, the strongest effect of GAG oligomerization was the amplified binding of IL‐8 monomers, which might concentrate monomeric protein in the extracellular matrix and thus promote protein dimerization under physiological conditions.

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Rational engineering of glycosaminoglycan-based Dickkopf-1 scavengers to improve bone regeneration

Cited by 2 publications

References 46 publications

Modulating Lineage Specification in Stem Cell Differentiation via Bioelectrical Stimulation Intensity Matching

Modulating Lineage Specification in Stem Cell Differentiation via Bioelectrical Stimulation Intensity Matching

Dimerization and Crowding in the Binding of Interleukin 8 to Dendritic Glycosaminoglycans as Artificial Proteoglycans

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