Rational engineering of phospholipase C from Bacillus cereus HSL3 for simultaneous thermostability and activity improvement

Zhang, Yonghui; Dai, Pengcheng; Liu, Rongkai; Liu, Wenlong; Xiao, Anfeng; Li, Jian; Li, Guiling; Li, Jingwen

doi:10.1016/j.jbiotec.2022.06.009

Cited by 5 publications

(3 citation statements)

References 44 publications

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“…The application of enzymes in the industrial synthesis of functional lipids may face challenges associated with high temperatures. For instance, elevated processing temperatures can simplify industrial degumming processes by reducing oil viscosity and mitigating microbial contamination, thereby facilitating enzymatic hydrolysis (Zhang et al 2022b ). The utilization of more thermostable enzymes in the industry has shown several benefits, such as improved biocatalytic efficiency, shortened processing times, and ultimately reduced energy consumption (Rathi et al 2016 ).…”

Section: Enhancing Functional Lipid Synthesis Via Protein Engineeringmentioning

confidence: 99%

“…The resulting variant, F96R/Q153P, exhibited a notable increase in its optimal reaction temperature (90 °C) and 2.37-fold enhancement in k cat / K m . The mutant shows great potential application in food processing industries such as enzymatic degumming under extreme high temperature(Zhang et al 2022b ). Substituting the N-terminal structural domain is another method for enhancing enzyme thermal stability, and this modification often brings surprising changes to the enzyme's functionality.…”

Section: Enhancing Functional Lipid Synthesis Via Protein Engineeringmentioning

confidence: 99%

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Enzyme engineering for functional lipids synthesis: recent advance and perspective

Guan,

Hou,

Yang

et al. 2024

Bioresour. Bioprocess.

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Functional lipids, primarily derived through the modification of natural lipids by various processes, are widely acknowledged for their potential to impart health benefits. In contrast to chemical methods for lipid modification, enzymatic catalysis offers distinct advantages, including high selectivity, mild operating conditions, and reduced byproduct formation. Nevertheless, enzymes face challenges in industrial applications, such as low activity, stability, and undesired selectivity. To address these challenges, protein engineering techniques have been implemented to enhance enzyme performance in functional lipid synthesis. This article aims to review recent advances in protein engineering, encompassing approaches from directed evolution to rational design, with the goal of improving the properties of lipid-modifying enzymes. Furthermore, the article explores the future prospects and challenges associated with enzyme-catalyzed functional lipid synthesis.

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Section: Enhancing Functional Lipid Synthesis Via Protein Engineeringmentioning

confidence: 99%

Section: Enhancing Functional Lipid Synthesis Via Protein Engineeringmentioning

confidence: 99%

Enzyme engineering for functional lipids synthesis: recent advance and perspective

Guan,

Hou,

Yang

et al. 2024

Bioresour. Bioprocess.

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show abstract

“…Additionally, PLCs exhibit the capacity to yield enhanced quantities of diacylglycerol (DAG). This augmentation in DAG content contributes to the overall oil yield, further underscoring the importance of PLCs in the context of oil degumming processes. , Therefore, PLCs have been considered to be one of the preferred enzymes for oil degumming.…”

Section: Introductionmentioning

confidence: 99%

Crystal Structure of Fungal Nonspecific Phospholipase C Unveils a Distinct Catalytic Mechanism

Feng,

Fang,

Wang

et al. 2023

J. Agric. Food Chem.

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Nonspecific phospholipase C (NPC) plays a pivotal role in hydrolyzing phospholipids, releasing diacylglycerolan essential second messenger. Extensive research has elucidated the structure and function of bacterial and plant NPCs, but our understanding of their fungal counterparts remains limited. Here, we present the first crystal structure of a fungal NPC derived from Rasamsonia emersonii (RePLC), unraveling its distinguishable features divergent from other known phospholipase C. Remarkably, the structure of RePLC contains solely the phosphoesterase domain without the crucial C-terminal domain (CTD) found in plant NPCs, although CTD is important for their activity. Through a comparative analysis of structural features among NPCs from diverse species combined with structure-based mutation analyses and bioinformatics methods, we propose a potential molecular mechanism that may universally underlie the catalysis of phospholipid hydrolysis in fungal NPCs. Furthermore, our study sheds light on the captivating evolutionary trajectory of enzymes across diverse species.

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AI-assisted food enzymes design and engineering: a critical review

Wang

Yang

Zhao

et al. 2022

Syst Microbiol and Biomanuf

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Rational engineering of phospholipase C from Bacillus cereus HSL3 for simultaneous thermostability and activity improvement

Cited by 5 publications

References 44 publications

Enzyme engineering for functional lipids synthesis: recent advance and perspective

Enzyme engineering for functional lipids synthesis: recent advance and perspective

Crystal Structure of Fungal Nonspecific Phospholipase C Unveils a Distinct Catalytic Mechanism

AI-assisted food enzymes design and engineering: a critical review

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