Rational orthologous pathway and biochemical process engineering for adipic acid production using Pseudomonas taiwanensis VLB120

Bretschneider, Lisa; Heuschkel, Ingeborg; Bühler, Katja; Karande, Rohan; Bühler, Bruno

doi:10.1016/j.ymben.2022.01.014

Cited by 18 publications

(15 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To enable complete lignocellulose consumption, non‐native catabolic pathways were incorporated in Pseudomonas strains, but several limitations remain such as slower growth and non‐ideal phenotypes (Elmore et al, 2020). The solvent tolerance of other P. putida group strains: S12, DOT‐T1E and P. taiwanensis VLB120 make them promising candidates for conversion of toxic substrates compared to the KT2440 strain (Bretschneider et al, 2022; Rühl et al, 2009). P. taiwanensis VLB120 also has a xylose utilization pathway that has been characterized, but it does not grow on p ‐coumarate and L‐arabinose, limiting its applications for growth on biomass hydrolysate (Wordofa & Kristensen, 2018).…”

Section: Discussionmentioning

confidence: 99%

Development of genetic tools for heterologous protein expression in a pentose‐utilizing environmental isolate of Pseudomonas putida

Gauttam

Eng

Zhao

et al. 2023

Microbial Biotechnology

View full text Add to dashboard Cite

Pseudomonas putida has emerged as a promising host for the conversion of biomass-derived sugars and aromatic intermediates into commercially relevant biofuels and bioproducts. Most of the strain development studies previously published have focused on P. putida KT2440, which has been engineered to produce a variety of non-native bioproducts. However, P. putida is not capable of metabolizing pentose sugars, which can constitute up to 25% of biomass hydrolysates. Related P. putida isolates that metabolize a larger fraction of biomass-derived carbon may be attractive as complementary hosts to P. putida KT2440. Here we describe genetic tool development for P. putida M2, a soil isolate that can metabolize pentose sugars. The functionality of five inducible promoter systems and 12 ribosome binding sites was assessed to regulate gene expression. The utility of these expression systems was confirmed by the production of indigoidine from C6 and C5 sugars. Chromosomal integration and expression of non-native genes was achieved by using chassis-independent recombinase-assisted genome engineering (CRAGE) for single-step gene integration of biosynthetic pathways directly into the genome of P. putida M2. These genetic tools provide a foundation to develop hosts complementary to P. putida KT2440 and expand the ability of this versatile microbial group to convert biomass to bioproducts. − , m k + ) supE44 thi −1 gyrA996 relA1 phoA Hanahan (1983) E. coli BW29427 A conjugal donor strain to transfer plasmids, auxotrophic to diaminopimelic acid Wang et al. (2019) P. putida KT2440 Wild type ATCC 12633 P. putida M2 Accession number JADOUD010000001 Park, Fong, et al. (2022) P. putida PPK1 (PP1; JPUB_014489) P. putida KT2440 carrying pRGPDuo2; Kan R Gauttam et al. (2020) P. putida PPK2 (PP2; JPUB_014491) P. putida KT2440 carrying pRGPDuo2-sfGFPtac RBS0; Kan R Gauttam et al. (2020) P. putida PPK3 (PP3; JPUB_014493) P. putida KT2440 carrying pRGPDuo2-sfGFPtet RBS0; Kan R Gauttam et al. (2020) P. putida PPK7 (PP7; JPUB_014775) P. putida KT2440 carrying pRGPDuo1; Gent R Gauttam et al. (2021) P. putida PPK9 (PP9; JPUB_014779) P. putida KT2440 carrying pRGPDuo1-sfGFPtac RBS0; Gent R Gauttam et al. (2021) P. putida PPK12 (PP12; JPUB_014785) P. putida KT2440 carrying pRGPDuo3; Gent R Gauttam et al. (2021) P. putida PPK13 (PP13; JPUB_014787) P. putida KT2440 carrying pRGPDuo4; Kan R Gauttam et al. (2021) P. putida PPK14 (PP14; JPUB_014810) P. putida KT2440 carrying pRGPDuo3-sfGFPbad RBS0; Gent R Gauttam et al. (2021) P. putida PPK16 (PP16; JPUB_014791) P. putida KT2440 carrying pRGPDuo4-sfGFPbad RBS0; Kan R Gauttam et al. (2021) P. putida PP2M226 (JPUB_020213) P. putida M2 carrying pRGPDuo1; Gent R This study P. putida PP2M227 (JPUB_020214) P. putida M2 carrying pRGPDuo2; Kan R This study P. putida PP2M229 (JPUB_020215) P. putida M2 carrying pRGPDuo1-sfGFPtac RBS0; Gent R This study P. putida PP2M230 P. putida M2 carrying pRGPDuo2-sfGFPtet RBS0; Kan R This study P. putida PP2M231 (JPUB_020216) P. putida M2 carrying pRGPDuo2-sfGFPtac RBS0; Kan R ...

show abstract

Section: Discussionmentioning

confidence: 99%

Development of genetic tools for heterologous protein expression in a pentose‐utilizing environmental isolate of Pseudomonas putida

Gauttam

Eng

Zhao

et al. 2023

Microbial Biotechnology

View full text Add to dashboard Cite

show abstract

“…Such biofilms can sometimes be beneficial for the environment and some industries. In fact, biofilms have been utilized for microbial bioprocessing, [36,37] microbial fuel cells, [38,39] bioremediation, [40,41] biosensor design, [42,43] and biohydrogen production. [44,45] However, a tendency of bacterial accumulation on surfaces is in charge of a diverse spectrum of local/systemic diseases and industrial biofouling.…”

Section: Discussionmentioning

confidence: 99%

A 3D‐Printed Customizable Platform for Multiplex Dynamic Biofilm Studies

Dhall

Ramjee

et al. 2022

Adv Materials Technologies

View full text Add to dashboard Cite

Biofilms are communities of microbes that colonize surfaces. While several biofilm experimental models exist, they often have limited replications of spatiotemporal dynamics surrounding biofilms. For a better understanding dynamic and complex biofilm development, this manuscript presents a customizable platform compatible with off‐the‐shelf well plates that can monitor microbial adhesion, growth, and associated parameters under various relevant scenarios by taking advantage of 3D printing. The system i) holds any substrate in a stable, vertical position, ii) subjects samples to flow at different angles, iii) switches between static and dynamic modes during an experiment, and iv) allows multiplexing and real‐time monitoring of biofilm parameters. Simulated fluid dynamics is employed to estimate flow patterns around discs and shear stresses at disc surfaces. A 3D printed peristaltic pump and a customized pH measurement system for real‐time tracking of spent biofilm culture media are equipped with a graphical user interface that grants control over all experimental parameters. The system is tested under static and dynamic conditions with Streptococcus mutans using different carbon sources. By monitoring the effluent pH and characterizing biochemical, microbiological, and morphological properties of cultured biofilms, distinct properties are demonstrated. This novel platform liberates designing experimental strategies for investigations of biofilms under various conditions.

show abstract

“…32 Recently, this route has been further amended by coupling with alcohol and aldehyde dehydrogenases for the synthesis of another important C6 bifunctional compound AA. 66…”

Section: -Hydroxyhexanoic Acid (Hha)mentioning

confidence: 99%

Recent advances in the sustainable production of α,ω-C6 bifunctional compounds enabled by chemo-/biocatalysts

Zhang

Zhao

et al. 2022

Green Chem.

View full text Add to dashboard Cite

show abstract

Rational orthologous pathway and biochemical process engineering for adipic acid production using Pseudomonas taiwanensis VLB120

Cited by 18 publications

References 64 publications

Development of genetic tools for heterologous protein expression in a pentose‐utilizing environmental isolate of Pseudomonas putida

Development of genetic tools for heterologous protein expression in a pentose‐utilizing environmental isolate of Pseudomonas putida

A 3D‐Printed Customizable Platform for Multiplex Dynamic Biofilm Studies

Recent advances in the sustainable production of α,ω-C6 bifunctional compounds enabled by chemo-/biocatalysts

Contact Info

Product

Resources

About