Rationally designed peptide nanosponges for cell-based cancer therapy

Peptide nanosponges designed for rapid uptake by leukocytes and neural stem cells

“…It is noteworthy that the nanosponges, obtained under the experimental conditions described here, appear to be larger (240 ± 30 nm in diameter), whereas their diameter reported earlier was 90 ± 15 nm. 38 However, a DK20 concentration approx. Five times lower (than reported in ref.…”

“…The preparation of PHK26-loaded type DK20 nanosponges was described earlier. 38 Cell experiments were carried out in the culturing medium described above. The percentage of viable cells was determined after 24 and 48 hours of incubation.…”

“…This team has recently reported the design, synthesis, and characterization of designer peptide-nanosponges for efficient uptake by delivery cells in drug delivery. 38 Their supramolecular building blocks consist of (cholesterol-(K/D) n DEVDGC) 3 -trimaleimide units featuring a trigonal maleimide linker to which either lysine (K) 20 or aspartic acid (D) 20 are attached. Furthermore, a consensus sequence for caspase-6 (DEVD-GC) 39 is integrated into the structures.…”

“…They are capable of incorporating fluorescent dyes ( e.g. carboxyfluorescein (discussed here) or PKH26 ( ref. 38 )), which can be used for fluorescence imaging and payload release studies.…”

Molecular simulations of self-assembling bio-inspired supramolecular systems and their connection to experiments

“…A possible reason is the difficulty to obtain reliable CG models for more complex molecules often involving conjugated ring systems. Exceptions are the CG self-assembly simulations on synthetic constructs from trimaleimide functionalized with peptide linkers and cholesterol, forming nanosponges, 109 and of porphyrin/dipeptide mixtures using DPD. 110 A recent highlight is the work of Bochicchio and Pavan who parameterized a Martini model for BTA.…”