Rationally designed protein cross-linked hydrogel for bone regeneration via synergistic release of magnesium and zinc ions

Chen, Xin; Tan, Baoyu; Wang, Shang; Tang, Rongze; Bao, Zhiteng; Chen, Gaoyang; Chen, Shuai; Tang, Wanze; Wang, Zhenmin; Long, Canling; Lu, William W.; Yang, Dazhi; Bian, Liming; Peng, Songlin

doi:10.1016/j.biomaterials.2021.120895

Cited by 72 publications

(44 citation statements)

References 50 publications

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“…Absorbable and bio-adhesive hydrogels could be injected or implanted locally at the defective site to facilitate the bone regeneration [ 10–12 ]. Hydrogel could also deliver pro-regenerative mineral salts including phosphate, zin ionic and silicate [ 13–15 ], to improve the osteogenic differentiation, alkaline phosphatase (ALP) activity and mineralization of human bone marrow stem cells (hBMSCs). Moreover, osteogenic cytokines, such as BMP-2, deferoxamine and stromal cell-derived factor-1 [ 16 ] or microRNA [ 17 ] that could improve the angiogenesis and osteogenesis, were also delivered in hydrogel to improve the efficacy of bone repair.…”

Section: Introductionmentioning

confidence: 99%

Titanium alloy composited with dual-cytokine releasing polysaccharide hydrogel to enhance osseointegration via osteogenic and macrophage polarization signaling pathways

Wang

Feng

Liu

et al. 2022

Regenerative Biomaterials

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Titanium alloy has been widely used in orthopedic surgeries as bone defect filling. However, the regeneration of high-quality new bones is limited due to the pro-inflammatory microenvironment around implants, resulting in a high occurrence rate of implant loosening or failure in osteological therapy. In this study, extracellular matrix (ECM)-mimetic polysaccharide hydrogel co-delivering BMP-2 and IL-4 was composited with 3D printed titanium alloy to promote the osseointegration and regulate macrophage response to create a pro-healing microenvironment in bone defect. Notably, it is discovered from the bioinformatics data that IL-4 and BMP-2 could affect each other through multiple signal pathways to achieve a synergistic effect towards osteogenesis. The composite scaffold significantly promoted the osteoblast differentiation and proliferation of human bone marrow mesenchyme stem cells (hBMSCs). The repair of large-scale femur defect in rat indicated that the dual-cytokine-delivered composite scaffold could manipulate a lower inflammatory level in situ by polarizing macrophages to M2 phenotype, resulting in superior efficacy of mature new bone regeneration over the treatment of native titanium alloy or that with an individual cytokine. Collectively, this work highlights the importance of M2-type macrophages-enriched immune-environment in bone healing. The biomimetic hydrogel-metal implant composite is a versatile and advanced scaffold for accelerating in vivo bone regeneration, holding great promise in treating orthopedic diseases.

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Section: Introductionmentioning

confidence: 99%

Titanium alloy composited with dual-cytokine releasing polysaccharide hydrogel to enhance osseointegration via osteogenic and macrophage polarization signaling pathways

Wang

Feng

Liu

et al. 2022

Regenerative Biomaterials

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“…Moreover, Chen et al [73] covalently cross-linked PEG with T4 lysozyme mutant (T4M), in order to fabricate hydrogels for Zn 2+ and Mg 2+ release. The cross-linking was based on surface-located free amine functional groups of T4M and was performed employing non-toxic covalent cross-linkers for solidifying the hydrogel matrix.…”

Section: Protein-based Hydrogelsmentioning

confidence: 99%

Protein-Based Hydrogels: Promising Materials for Tissue Engineering

et al. 2022

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The successful design of a hydrogel for tissue engineering requires a profound understanding of its constituents’ structural and molecular properties, as well as the proper selection of components. If the engineered processes are in line with the procedures that natural materials undergo to achieve the best network structure necessary for the formation of the hydrogel with desired properties, the failure rate of tissue engineering projects will be significantly reduced. In this review, we examine the behavior of proteins as an essential and effective component of hydrogels, and describe the factors that can enhance the protein-based hydrogels’ structure. Furthermore, we outline the fabrication route of protein-based hydrogels from protein microstructure and the selection of appropriate materials according to recent research to growth factors, crucial members of the protein family, and their delivery approaches. Finally, the unmet needs and current challenges in developing the ideal biomaterials for protein-based hydrogels are discussed, and emerging strategies in this area are highlighted.

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“…[130] In another application, Chen et al developed a recombinant protein crosslinked hydrogel loaded with magnesium and zinc ions for bone regeneration via their synergistic and sustained release. [131] Glycol chitosan, gelatin, silk fibroin, alginate, and cell-laden based hydrogels have also been studied for bone tissue engineering. [132][133][134][135][136][137][138] The development of pH responsive injectable hydrogels have been shown to be good candidates for localized and sustained delivery of RNAi.…”

Section: Other Prophylactic Treatment Examplesmentioning

confidence: 99%

Hydrogels and Hydrogel Nanocomposites: Enhancing Healthcare through Human and Environmental Treatment

Gutiérrez

Frazar

Klaus

et al. 2021

Adv Healthcare Materials

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Humans are constantly exposed to exogenous chemicals throughout their life, which can lead to a multitude of negative health impacts. Advanced materials can play a key role in preventing or mitigating these impacts through a wide variety of applications. The tunable properties of hydrogels and hydrogel nanocomposites (e.g., swelling behavior, biocompatibility, stimuli responsiveness, functionality, etc.) have deemed them ideal platforms for removal of environmental contaminants, detoxification, and reduction of body burden from exogenous chemical exposures for prevention of disease initiation, and advanced treatment of chronic diseases, including cancer, diabetes, and cardiovascular disease. In this review, three main junctures where the use of hydrogel and hydrogel nanocomposite materials can intervene to positively impact human health are highlighted: 1) preventing exposures to environmental contaminants, 2) prophylactic treatments to prevent chronic disease initiation, and 3) treating chronic diseases after they have developed.

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Rationally designed protein cross-linked hydrogel for bone regeneration via synergistic release of magnesium and zinc ions

Cited by 72 publications

References 50 publications

Titanium alloy composited with dual-cytokine releasing polysaccharide hydrogel to enhance osseointegration via osteogenic and macrophage polarization signaling pathways

Titanium alloy composited with dual-cytokine releasing polysaccharide hydrogel to enhance osseointegration via osteogenic and macrophage polarization signaling pathways

Protein-Based Hydrogels: Promising Materials for Tissue Engineering

Hydrogels and Hydrogel Nanocomposites: Enhancing Healthcare through Human and Environmental Treatment

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