Ratlarda Metoraksat Kaynaklı Testiküler Toksisite Üzerine Hesperidinin Etkisi

Abstract

Citation of This ArticleBelhan S, Özkaraca M, Kandemir FM, Gülyüz F, Yıldırım S, Ömür AD, Yener Z: Effectiveness of hesperidin on methotrexate-induced testicular toxicity in rats. Kafkas Univ Vet Fak Derg, 23 (5): 789-796, 2017789-796, . DOI: 10.9775/kvfd.2017 AbstractThe aim of this study was to investigate the effect of hesperidin on male reproductive system in rats to which methotrexate (MTX) was administered. In the study, 28 male Wistar albino rats at the age of 8 weeks and had 250-300 g of live weight were used. Four experimental groups were formed; Group 1 (n=7): The control group, only feed and water were given. Group 2 (n=7): MTX group, a single dose of 20 mg/ kg of i.p. MTX was administered. Group 3 (n=7): Hesperidin group, 200 mg/kg of hesperidin was administered by gavage for 7 days. Group 4: MTX + hesperidin group (n=7): Following administration of a single dose of 20 mg/kg i.p. MTX , 200 mg/kg of Hesperidin was administered by oral gavage for 7 days. At the end of the experiment, rats were decapitated and biochemical, histopathological and spermatological parameters were examined. It was observed that in the MTX group, sperm motility and density, the enzymes CAT, GPx and SOD and GSH level decreased, TNF-alpha and IL-1 Beta, as well as MDA, levels were increased, regular structure of spermatogenic cells was impaired, and seminiferous tubules became necrotic and degenerative. It was determined that spermatological parameters improved and, necrotic and degenerative changes diminished by the administration of MTX+hesperidin. These outcomes indicated that hesperidin had a protective effect on destructive effects of MTX in rat testiclesv. Keywords: Male rat, Hesperidin, Methotrexate, Sperm, Reproductive system Ratlarda Metoraksat Kaynaklı Testiküler Toksisite Üzerine Hesperidinin Etkisi ÖzetBu çalışmanın amacı, metoraksat (MTX) uygulanan ratlarda hesperidinin erkek üreme sistemi üzerindeki etkisini araştırmaktı. Çalışmada 8 haftalık yaşta, 250-300 g canlı ağırlığa sahip 28 erkek Wistar albino cinsi rat kullanıldı. Dört deney grubu oluşturuldu; Grup 1 (n=7): kontrol grubu, sadece yem ve su verildi. Grup 2 (n=7): MTX grubu, 20 mg/kg MTX i.p. tek doz uygulandı. Grup 3 (n=7): Hesperidin grubu, 200 mg/kg hesperidin 7 gün süre ile ağızdan sonda yardımıyla verildi. Grup 4 (n=7): MTX + hesperidin grubu, 20 mg/kg MTX i.p tek doz uygulamasını takiben 200 mg/kg hesperidin 7 gün süre ile ağızdan sonda yardımıyla verildi. Deney sonunda ratlar dekapite edilerek biyokimyasal, histopatolojik ve spermatolojik parametreler incelendi. MTX grubunda sperm motilitesi ve yoğunluğunun, CAT, GPx ve SOD enzimlerinin ve GSH düzeyinin düştüğü, TNF-alpha ve IL-1 Beta ile MDA düzeyinin arttığı, spermatojenik hücrelerin düzenli yapısının bozulduğu, seminifer tubullerin nekrotik ve dejeneratif bir hal aldığı gözlendi. MTX + hesperidin uygulaması ile spermatolojik parametrelerin düzeldiği, nekrotik ve dejeneratif değişikliklerin azaldığı tespit edildi. Bu sonuçlar hesperidinin rat testislerinde MTX tarafından oluşan yıkıcı etkiler üze...

Investigation of protective effects of lithium borate on spermatogenesis and testes histopathology against cadmium-induced acute toxicity in rats

“…8-OHdG expressions in the Cd group were severely observed. The expression of 8-OHdG in this toxicity is consistent with the literature (Belhan et al, 2017;Belhan et al, 2019).…”

“…8-OHdG concentrations of a cell reveal a significant measure of oxidative stress. The 8-OHdG expressions have been reported to be severe in testis tissue in oxidative damage (Belhan et al, 2017;Saripinar et al, 2017). In this study, 8-OHdG expressions were immunohistochemically observed to determine the oxidative damage in testes.…”

Ginkgo biloba Extract Attenuates Methotrexate-Induced Testicular Injury in Rats: Cross-talk Between Oxidative Stress, Inflammation, Apoptosis, and miRNA-29a Expression

“…NFκB is thus considered an impor tant regulator of inflammation. 13 MTX was reported to induce a marked rise in the inflammatory mediators like IL6 and IL1β in testicular tissues 10,13,15,39 along with upregulation of the NFκB mRNA and protein testicular expressions. 13,15 In our study, MTX induced a marked testicular inflammation response which was confirmed by the marked elevation in the inflammatory markers of IL6 and IL1β testicular levels as well as the marked upregulation of the testicular NFκB expression, which was in harmony with previous studies.…”

“…Moreover, the release of pro-inflammatory cytokines like interleukin-6 (IL-6) and IL-1β along with cell death through apoptotic cascades was also documented in the pathophysiology of MTX-induced testicular injury. 10,13,15 Apoptosis is known as a process of programmed cell death and regulated by the family members of bcl-2 which has been documented to be involved in the testicular apoptosis. 16 -18 Bcl-2 has been reported to inhibit apoptosis and prolong cell survival while bcl-2 associated x protein (Bax) could block the capability of bcl-2 in inhibiting apoptosis and could be considered as an apoptosis regulator.…”

Protective effects of apocynin on damaged testes of rats exposed to methotrexate

“…Adding an antioxidant medication to MTX treatment regimen may be the solution to reduce or prevent the side effects of MTX. Many researchers have studied effect of different substances such as against oxidative damage of MTX in testes [10,11,18,31,34,[37][38][39][40][41][42][43][44]. MTX + caffeic acid phenethyl ester (CAPE) treatment caused the oxidative stress to increase in the testicles despite the antioxidant, antiinflammatory properties of CAPE [31].…”