Rats recognize spatial and temporal attributes in a new object recognition memory task with multiple trials

Araujo, Ana Paula de Castro; Lucena, Jalles Dantas de; Drieskens, Davi Carvalho; Neves, Lívia Rodrigues; Pugliane, Karen Cristina; Belchior, Hindiael; Castelo-Branco, Rochele; Barbosa, Flávio Freitas

doi:10.1016/j.jneumeth.2020.108936

Cited by 4 publications

(2 citation statements)

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“…We considered as object exploration time intervals when a rat faced an object at least 2 centimeters away from its snout for more than 1 s. In order to reduce locomotion-related modulation of hippocampal oscillations, the following analyses have only included behavioral epochs of object exploration in which the animals were clearly still and not running or walking. The total exploration time was then calculated as the sum of the time spent exploring each object cumulatively minute-by-minute within each 5-min session (sample, HD and LD tests), similarly to previous studies ( Dix and Aggleton, 1999 ; Ameen-Ali et al, 2015 ; Araujo et al, 2021 ). In order to test novel object location recognition memory, we used a discrimination index that evaluates the animal’s spontaneous preference for one of the objects.…”

Section: Methodsmentioning

confidence: 99%

“…The animals are allowed to explore two equal objects in a familiar arena during the sample phase, and after a given interval one of these objects is moved to a new location. It is expected that rats spend more time exploring the displaced object relative to the stationary one ( Ennaceur, 2010 ; Cohen and Stackman, 2015 ; Araujo et al, 2021 ). Hunsaker and Kesner (2008) have developed a NOL paradigm that sets different levels of object displacement as a tool to study spatial pattern separation.…”

Section: Introductionmentioning

confidence: 99%

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Theta and gamma oscillations in the rat hippocampus support the discrimination of object displacement in a recognition memory task

Neves

Lobão-Soares

Araujo

et al. 2022

Front. Behav. Neurosci.

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Episodic memory depends on the recollection of spatial and temporal aspects of past experiences in which the hippocampus plays a critical role. Studies on hippocampal lesions in rodents have shown that dentate gyrus (DG) and CA3 are necessary to detect object displacement in memory tasks. However, the understanding of real-time oscillatory activity underlying memory discrimination of subtle and pronounced displacements remains elusive. Here, we chronically implanted microelectrode arrays in adult male Wistar rats to record network oscillations from DG, CA3, and CA1 of the dorsal hippocampus while animals executed an object recognition task of high and low spatial displacement tests (HD: 108 cm, and LD: 54 cm, respectively). Behavioral analysis showed that the animals discriminate between stationary and displaced objects in the HD but not LD conditions. To investigate the hypothesis that theta and gamma oscillations in different areas of the hippocampus support discrimination processes in a recognition memory task, we compared epochs of object exploration between HD and LD conditions as well as displaced and stationary objects. We observed that object exploration epochs were accompanied by strong rhythmic activity in the theta frequency (6–12 Hz) band in the three hippocampal areas. Comparison between test conditions revealed higher theta band power and higher theta-gamma phase-amplitude coupling in the DG during HD than LD conditions. Similarly, direct comparison between displaced and stationary objects within the HD test showed higher theta band power in CA3 during exploration of displaced objects. Moreover, the discrimination index between displaced and stationary objects directly correlated with CA1 gamma band power in epochs of object exploration. We thus conclude that theta and gamma oscillations in the dorsal hippocampus support the successful discrimination of object displacement in a recognition memory task.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Theta and gamma oscillations in the rat hippocampus support the discrimination of object displacement in a recognition memory task

Neves

Lobão-Soares

Araujo

et al. 2022

Front. Behav. Neurosci.

Self Cite

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Post-learning caffeine administration improves ‘what-when’ and ‘what-where’ components of episodic-like memory in rats

Dias

Golzio

Santos

et al. 2022

Behavioural Brain Research

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Assessing episodic memory in rodents using spontaneous object recognition tasks

Barbosa

Castelo-Branco

2022

Emerging Topics in Life Sciences

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Models of episodic memory are successfully established using spontaneous object recognition tasks in rodents. In this review, we present behavioral techniques devised to investigate this type of memory, emphasizing methods based on associations of places and temporal order of items explored by rats and mice. We also provide a review on the areas and circuitry of the medial temporal lobe underlying episodic-like memory, considering that a large number of neurobiology data derived from these protocols. Although spontaneous recognition tasks are commonplace in this field, there is need for careful evaluation of factors affecting animal performance. Such as the ongoing development of tools for investigating the neural basis of memory, efforts should be put in the refinement of experimental designs, in order to provide reliable behavioral evidence of this complex mnemonic system.

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Rats recognize spatial and temporal attributes in a new object recognition memory task with multiple trials

Cited by 4 publications

References 23 publications

Theta and gamma oscillations in the rat hippocampus support the discrimination of object displacement in a recognition memory task

Theta and gamma oscillations in the rat hippocampus support the discrimination of object displacement in a recognition memory task

Post-learning caffeine administration improves ‘what-when’ and ‘what-where’ components of episodic-like memory in rats

Assessing episodic memory in rodents using spontaneous object recognition tasks

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