2018
DOI: 10.1016/j.cbpc.2017.10.008
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Rattling the border wall: Pathophysiological implications of functional and proteomic venom variation between Mexican and US subspecies of the desert rattlesnake Crotalus scutulatus

Abstract: While some US populations of the Mohave rattlesnake (Crotalus scutulatus scutulatus) are infamous for being potently neurotoxic, the Mexican subspecies C. s. salvini (Huamantlan rattlesnake) has been largely unstudied beyond crude lethality testing upon mice. In this study we show that at least some populations of this snake are as potently neurotoxic as its northern cousin. Testing of the Mexican antivenom Antivipmyn showed a complete lack of neutralisation for the neurotoxic effects of C. s. salvini venom, w… Show more

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Cited by 33 publications
(32 citation statements)
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“…Fluorescent substrate cleavage studies were conducted as previously described by us (Cipriani et al, 2017;Debono et al, 2017;Dobson et al, 2017;Koludarov et al, 2017). A working stock solution of freeze-dried venom was reconstituted in a buffer containing 50% deionised H2O/50% glycerol (>99.9%, Sigma) at a 1:1 ratio to preserve enzymatic activity and reduce enzyme degradation with the final venom concentration of 0.1 mg/ml, and then stored at -20°C.…”
Section: Cleavage Of Fluorescent Substratesmentioning
confidence: 99%
“…Fluorescent substrate cleavage studies were conducted as previously described by us (Cipriani et al, 2017;Debono et al, 2017;Dobson et al, 2017;Koludarov et al, 2017). A working stock solution of freeze-dried venom was reconstituted in a buffer containing 50% deionised H2O/50% glycerol (>99.9%, Sigma) at a 1:1 ratio to preserve enzymatic activity and reduce enzyme degradation with the final venom concentration of 0.1 mg/ml, and then stored at -20°C.…”
Section: Cleavage Of Fluorescent Substratesmentioning
confidence: 99%
“…Ecological niche partitioning and a shift in diet would contribute to venom evolution of the species across varying localities and habitats, with consequential impacts upon relative clinical effects Fry et al, 2008). Such wide variation depicted within a single genus has also been shown within many other groups of closely related snakes, each inhabiting slightly varied ecological niches (Debono et al, 2019a;Debono et al, 2019c;Debono et al, 2017a;Dobson et al, 2018;Rogalski et al, 2017) as well as variation between localities of the same species (Sousa et al, 2018). While some ontogenetic shift has been shown within one species of Gloydius (Gao et al, 2013), due to lack of sample availability the impact upon relative coagulotoxicity was not investigated in the present study and should be the subject of future work.…”
Section: Discussionmentioning
confidence: 60%
“…Venoms from Azemiops feae, Calloselasma rhodostoma, Deinagkistrodon acutus, Hypnale hypnale, Tropidolaemus subannulatus and Tropidolaemus wagleri were investigated for ability to cleave fibrinogen using protocols previously described by us (Debono et al, 2019c;Dobson et al, 2018).…”
Section: Fibrinogen Sds Page Electrophoresis and Gel Image Analysismentioning
confidence: 99%
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