Rauscher-leukemia-virus-related sequences in human DNA: presence in some tissues of some patients with hemotopoietic neoplasias and absence in DNA from other tissues.

Abstract

Nature 262,[190][191][192][193][194][195], because there is no sequence homology between nucleic acids from MuLVR and baboon virus. The absence of these nucleic acid sequences in many tissues of patients with neoplasia and from the few tissues examined from people with nonneoplastic disease suggests that they are not endogenous elements but are acquired after fertilization. Taken together with the previous detection of baboon and woolly monkey type-C viral related components in some human tumors, the results suggest acquisition of at least three types of type-C viral sequences in the human population. Data are accumulating that indicate the presence of type-Ctumor-virus-like components in some human tissues (1-23). Cytoplasmic nucleic acids with sequences related to the RNA of Rauscher murine leukemia virus (MuLVR) and other viruses have been described (4, 9-17). Recently, nucleic acids and proteins related to the two known primate type-C virus groups-the simian sarcoma virus/gibbon ape leukemia virus group and the baboon endogenous virus group-have been reported in fresh human tissues (5-8, 11-14, 16, 18-23) The objective of experiments reported here was to examine DNA from human tissues for sequences related to RNA of MuLVR in more detail and with more carefully prepared cDNA probes. We report: (i) detection of nucleic acid sequences distantly related to MuLVR in DNA from spleens of some patients with acute myelogenous leukemia (AML), Hodgkin's disease, and multiple myeloma; (ii) absence of these sequences in DNA from apparently uninvolved tissues of the same patients with neoplasia and from the normal human tissues tested. Since MuLVR and the baboon endogenous virus are not detectably related (32), the detection of MuLVR-related DNA sequences is not explained by the previously reported presence of proviral sequences of baboon virus in DNA from some leukemia patients. Taken together, the results show at least two independent sets of oncornavirus-related sequences in the DNA from some leukemia patients. While the mode, source, and time of acquisition, and relevance to etiology, must be speculative, interspecies transmission of one or both agents may be involved.MATERIALS AND METHODS Purification of DNA from Human Tissues. The tissues used in these experiments were uncultured specimens obtained at the time of autopsy and kept frozen at -80°until the isolation of DNA. The organ tissues obtained from people with neoplasia were infiltrated with tumor cells. The tissues were lysed in a

Virus-induced Diabetes Mellitus. XXV. Difference in the RNA Fingerprints of Diabetogenic and Non-diabetogenic Variants of Encephalomyocarditis Virus

“…The hybrids were also analysed for thermal stability to evaluate mismatching in base pairing. Thermal elution midpoints (te50) were determined by loading hydroxylapatite columns with the hybrids in 0.12M-phosphate buffer pH 6-8 containing 0.2~ SDS at 60°C and subsequent elution with the same buffer at 4 °C increments up to 100 °C (Aulakh & Gallo, 1977). As shown in Fig.…”

Search for type‐c oncornavirus–related genetic information in tissues from patients with systemic lupus erythematosus

“…In particular, type-C-virus-like particles were found in placentas of both SLE patients and normal women (38,39), and virus isolation attempts from SLE tissues have been negative (40,41) with one possible exception (42). More definitive evidence of type-C virus presence would be provided by finding DNA provirus copies in SLE tissues (1)(2)(3)(4)(5)(6)14). We therefore addressed the question of type-C involvement in SLE by hybridizing DNA from various tissues with recycled DNA probes that were made from two type-C strains related to those implicated by immunochemical studies (32)(33)(34)(35)(36).…”

“…Various strains of type-C oncornaviruses are widespread among species of the animal kingdom and there are a number of reports implicating their presence in human beings. Several laboratories have reported evidence associating type-C oncornavirus with human malignancies by the presence of viral coat proteins, reverse transcriptase, and provirus DNA sequences (DNA copies of the viral RNA genome) in these tissues (7)(8)(9)(10)(11)(12)(13)(14). Isolations of type-C oncornaviruses from cultured human tissues have been reported (15)(16)(17)(18)(19), but are clearly uncommon (20).…”

DNA Relatedness among Established Ureaplasma Species and Unidentified Feline and Canine Serogroups

“…The sheared [3H]DNA was then extracted with phenol saturated with TNE buffer (10 mM NaCl, 1 mM EDTA, 10 mM Tris-hydrochloride; pH 7), dialyzed against TNE buffer, and concentrated by lyophilization. has been described previously (1). Briefly, the reaction mixture contained 1 mg of unlabeled, sheared DNA per ml, 15,000 cpm of 3H-labeled DNA probe, 0.2% SDS, 1 mM EDTA, and 0.48 M phosphate buffer.…”

“…A 2-1-1.8 portion of purified native DNA was labeled in vitro with [1',2',5'-3H]dCTP (Amersham Corp., Arlington Heights, Ill.) by the nick translation method (17) and was processed for hybridization as described previously (1). The specific activity of the resultant single-stranded [3H]DNA probes ranged from 1.0 x lo7 to 2.0 x lo7 cprn/pg of DNA, as determined by trichloroacetic acid precipitation.…”