Raw and thermally treated cement asbestos exerts different cytotoxicity effects on A549 cells in vitro

Pugnaloni, Armanda; Lucarini, Guendalina; Rubini, Corrado; Smorlesi, Arianna; Tomasetti, Marco; Strafella, Elisabetta; Armeni, Tatiana; Gualtieri, Alessandro

doi:10.1016/j.acthis.2014.10.007

Cited by 5 publications

(5 citation statements)

References 48 publications

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“…As mentioned, KRY·AS is the product of the thermal inertization of CAS performed in industrial kilns at temperatures in the range of 1200–1300 °C . The obtained product is free of asbestos phases and contains newly formed clinker phases with cytotoxic effects comparable to those of commercial clinker . More specifically, KRY·AS is composed of subspherical particles of the newly formed crystalline phases but with an excess of aluminum, iron, and magnesium and a compositional variability mostly depending on the provenience of the CAS .…”

Section: Methodsmentioning

confidence: 99%

“…7 The obtained product is free of asbestos phases and contains newly formed clinker phases with cytotoxic effects comparable to those of commercial clinker. 9 More specifically, KRY•AS is composed of subspherical particles of the newly formed crystalline phases but with an excess of aluminum, iron, and magnesium and a compositional variability mostly depending on the provenience of the CAS. 10 Glass powder is from the sleeve filters of the mills used to grind glass from domestic recycling.…”

Section: Methodsmentioning

confidence: 99%

“…Disposal in landfills cannot be the ultimate solution not only as it conflicts with the directives of the European Commission (2008/98/EC, end-of-waste concept) but also as zero risk of fiber dispersion in air and water in the long run cannot be guaranteed . A very promising alternative to landfill storage is the inertization achieved through thermal treatment as firing of CAS yields an inert fine material, generally referred to as KRY·AS. − KRY·AS is an end-of-waste and has been proposed as a replacement for NRM in various applications depending on its mineralogical and chemical composition, demonstrating that the thermal inertization and recycling of the product of transformation are an effective solution to the asbestos problem. , …”

Section: Introductionmentioning

confidence: 99%

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Al-Substituted Tobermorites: An Effective Cation Exchanger Synthesized from “End-of-Waste” Materials

et al. 2022

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The policies to meet the “zero waste” regime and transition to sustainable circular economy can no longer ignore the use of wastes in place of natural resources, and these daunting and vital societal challenges are nowadays being faced by several nations. The main objective of this work was to search waste materials suitable for a quick and environmentally friendly production of a nanoporous geomaterial able to trap toxic metals at the solid/liquid interface. More specifically, the end-of-waste from the thermal inertization of cement–asbestos and glass powder from domestic glass containers have been employed as sources for the hydrothermal synthesis of a tobermorite-rich material (TRM) successfully tested for the selective removal of Pb2+, Zn2+, Cd2+, and Ni2+ from aqueous solutions. The synthesis was carried out in alkaline solution under mild hydrothermal conditions (120 °C) within 24 h. The quantitative phase analyses of the TRM carried out by applying the Rietveld method showed the occurrence of a large amount of well-crystallized 11 Å Al-substituted tobermorites and an amorphous phase and a lower content of aragonite and calcite. Chemical analyses and thermogravimetric measurements coupled with simultaneous evolved gas mass spectrometry highlighted that Al3+ for Si4+ substitutions in the wollastonite-like tetrahedral chains of tobermorites are balanced by the occurrence of Ca2+, Na+, and K+ cations in the interlayer rather than by (OH)− for O2– substitutions in the CaO polyhedra. Time-dependent removal tests clearly indicated that metal cations are selectively adsorbed depending on their concentration in solution. Moreover, the kinetic curves showed that the removal of metals from solution is fast and the equilibrium is almost reached in the first 8 h.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Al-Substituted Tobermorites: An Effective Cation Exchanger Synthesized from “End-of-Waste” Materials

et al. 2022

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“…Treatments were performed in triplicate. This experimental configuration was chosen because it allows a good interaction fibre/cell in culture systems and produces well-defined cellular responses as reported in previous studies (Hamilton et al, 1996;Cardile et al, 2004Cardile et al, , 2007aCardile et al, , 2007bPugnaloni et al, 2010Pugnaloni et al, , 2015Gualtieri et al, 2019c).…”

Section: Alamar Blue Viability Assaymentioning

confidence: 99%

“…Furthermore, in addition to the Met-5A and THP-1 cell lines, the human non-small cell lung carcinoma A549 cell line was tested. A549 cell line was employed in several studies as a cell model for ROS assay (Cardile et al, 2004;Pugnaloni et al, 2015;Wang et al, 2019). As shown by Cardile et al (2004), A549 cells produce a well-defined cellular response after exposure to fibrous amphiboles (such as crocidolite and fluoro-edenite) and ROS production is dose dependent.…”

Section: Reactive Oxygen Speciesmentioning

confidence: 99%

In vitro toxicity of fibrous glaucophane

et al. 2021

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The health hazard represented by the exposure to asbestos may also concern other minerals with asbestos-like crystal habit. One of these potentially hazardous minerals is fibrous glaucophane. Fibrous glaucophane is a major component of blueschist rocks of California (USA) currently mined for construction purposes. Dust generated by the excavation activities might potentially expose workers and the general public. The aim of this study was to determine whether fibrous glaucophane induces in vitro toxicity effects on lung cells by assessing the biological responses of cultured human pleural mesothelial cells (Met-5A) and THP-1 derived macrophages exposed for 24 h and 48 h to glaucophane fibres. Crocidolite asbestos was tested for comparison. The experimental configuration of the in vitro tests included a cell culture without fibres (i.e., control), cell cultures treated with 50 μg/mL (i.e., 15.6 μg/cm 2 ) of crocidolite fibres and 25-50− 100 μg/mL (i.e., 7.8− 15.6-31.2 μg/cm 2 ) of glaucophane fibres. Results showed that fibrous glaucophane may induce a decrease in cell viability and an increase in extra-cellular lactate dehydrogenase release in the tested cell cultures in a concentration dependent mode. Moreover, it was found that fibrous glaucophane has a potency to cause oxidative stress. The biological reactivity of fibrous glaucophane confirms that it is a toxic agent and, although it apparently induces lower toxic effects compared to crocidolite, exposure to this fibre may be responsible for the development of lung diseases in exposed unprotected workers and population.

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Mechanisms of action of mineral fibres in a placental syncytiotrophoblast model: An in vitro toxicology study

Fantone,

Tossetta,

Cianfruglia

et al. 2024

Chemico-Biological Interactions

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Raw and thermally treated cement asbestos exerts different cytotoxicity effects on A549 cells in vitro

Cited by 5 publications

References 48 publications

Al-Substituted Tobermorites: An Effective Cation Exchanger Synthesized from “End-of-Waste” Materials

Al-Substituted Tobermorites: An Effective Cation Exchanger Synthesized from “End-of-Waste” Materials

In vitro toxicity of fibrous glaucophane

Mechanisms of action of mineral fibres in a placental syncytiotrophoblast model: An in vitro toxicology study

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