Raxofelast, a hydrophilic vitamin E-like antioxidant, stimulates wound healing in genetically diabetic mice

Galeano, Mariarosaria; Torre, Valerio; Deodato, Barbara; Campo, Giuseppe M.; Mr, Colonna; Sturiale, Alessio; Squadrito, Francesco; Cavallari, Vittorio; Cucinotta, Domenico; Buemi, Michele; Altavilla, Domenica

doi:10.1067/msy.2001.112072

Cited by 48 publications

(41 citation statements)

References 26 publications

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“…31,52 Erhan et al 53 suggest that both vitamin E and selenium promote wound healing activity by increasing the blood flow in the wound region. New evidences from the studies on diabetic mouse models point to an involvement of oxidative stress in diabetic wound healing and significantly improved wound healing by topical vitamin E. 54,55 Another finding showed vitamin E or α-tocopherol supplementation reduced the levels of lipid peroxides in the wound tissue of diabetic rats, and the wound healing process was enhanced, in particular in the Vitamin E treated animals. 56 Tsoureli-Nikita et al 57 performed a clinical single, blind, placebo controlled study in which 96 atopic dermatitis patients were treated with either placebo or oral vitamin E (400 IE/day) for 8 months.…”

Section: Discussionmentioning

confidence: 98%

Cutaneous Wound Healing after Topical Application of <i>Pistacia atlantica</i> Gel Formulation in Rats

Hamidi

Naeini

Oryan

et al. 2017

tjps

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ÖZAmaç: Bu çalışma, sıçanlarda oluşturulan deneysel yara modelinde Pistacia atlantica yağı ile hazırlanmış jel formülasyonunun, enzimatik antioksidanlar üzerindeki etkisini araştırmak amacıyla yapılmıştır. Gereç ve Yöntemler: Genel anestezi altına alınan hayvanlarda, aseptik koşullarda, insizyon yapmak suretiyle kare şeklinde yaralar (2x2 cm) oluşturulmuştur. Daha sonra, hayvanlar rasgele dört gruba ayrılmıştır (I-tedavi edilmemiş kontrol grubu, II-haricen uygulanan baz jel grubu, IIIharicen %5 jel uygulanan grup, IV-haricen %10 jel uygulanan grup). Bulgular: Sonuçlar, Pistacia atlantica yağı ile hazırlanmış jel formülasyonunun haricen uygulanmasının, kontrol ve baz jel gruplarına kıyasla, bütünlüğü bozulmamış stratum bazalisi, olgun granülasyon dokusunu, kıl folikülleri ve ter bezleri ile reepitelizasyonu arttırdığını göstermektedir. Yara oluşumundan 21 gün sonra, %10 yağlı jel ile tedavi edilen hayvanların dokularındaki gerilme kuvveti, kopma gerilimi ve bükülme direnci, kontrol ve baz jel grubu ile karşılaştırıldığında önemli ölçüde artmıştır. Aynı evredeki kontrol ve baz jel ile muamele edilen gruplara kıyasla, kollajen liflerin daha organize bir düzenlenme ve dokunun daha düzgün bir iyileşme gösterdiği belirlenmiştir. Sonuç: Pistacia atlantica yağ jelinin haricen uygulanmasının, sıçanlarda oluşturulan deneysel yaralar üzerinde morfolojik, biyokimyasal ve biyomekanik yönden düzeltici rol oynadığı ortaya koyulmuştur. Anahtar kelimeler: Pistacia atlantica, yara iyileşmesi, histopatoloji, biomekanik, deri Objectives: This study has been undertaken to investigate the antioxidant activity of the gel formulation from Pistacia atlantica oil extraction on enzymatic antioxidants in experimental wound created in rats. Materials and Methods: Under anesthesia, a square-shaped skin defect (2x2 cm) was created aseptically by surgical incision. Then, animals were randomly allocated to four groups (I-untreated controls, II-topically treated with base gel, III-topically treated with 5% gel, IV-topically treated with 10% gel). Results:The results suggest that topical application of Pistacia atlantica oil gels improved reepithelialization with continuous stratum basalis and a mature granulation tissue and adnexa (hair follicles and sweat gland) compared with control and base gel groups. Treatment with 10% oil gel significantly enhanced the tensile strength, ultimate stress, yield strength and stiffness in this group compared with the control and base gel groups at 21 days post injury. The collagen fibers showed a more organized pattern and the tissue alignment was greater as compared to the control and base gel-treated groups at the same stage. Conclusion:The results suggest that topical application of Pistacia atlantica oil gel improved the morphological, biochemical and biomechanical properties of experimentally-induced wound defects in rats.

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Section: Discussionmentioning

confidence: 98%

Cutaneous Wound Healing after Topical Application of <i>Pistacia atlantica</i> Gel Formulation in Rats

Hamidi

Naeini

Oryan

et al. 2017

tjps

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“…19 In incisional skin-wound models made on the back of db/db mice, delayed repair was characterized by reduced angiogenesis, delayed formation of granulation tissue, decreased collagen content, and low breaking strength as compared with normal littermates. 20 Characteristics of the microvascular derangements during healing in a mouse model of diabetes include decreased arteriolar number and density, loss of vascular tone, and a reduction in the cross-sectional area of new vessel walls. 20 In a study by Altavilla et al, greater conjugated diene content, a measure of the extent of lipid peroxidation, was associated with wounds in mouse models of diabetes.…”

Section: Impaired Wound Healingmentioning

confidence: 99%

Abnormal angiogenesis in diabetes mellitus

Martin

Komada

Sane

2002

Medicinal Research Reviews

413

274

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The adverse long-term effects of diabetes mellitus have been well described and involve many organ systems. While diabetes management has largely focused on control of hyperglycemia, the presence of abnormalities of angiogenesis may cause or contribute to many of the clinical manifestations of diabetes. When compared with non-diabetic subjects, diabetics demonstrate vascular abnormalities of the retina, kidneys, and fetus. Diabetics have impaired wound healing, increased risk of rejection of transplanted organs, and impaired formation of coronary collaterals. In each of these conditions, and possibly in diabetic neuropathy as well, abnormalities of angiogenesis can be implicated in the pathogenesis. A perplexing feature of the aberrant angiogenesis is that excessive and insufficient angiogenesis can occur in different organs in the same individual. In this review, the clinical features, molecular mechanisms, and potential therapeutic options of abnormal angiogenesis in diabetes will be reviewed.

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“…[3][4][5] Consistent with this notion, previous studies have shown that antioxidants accelerated diabetic wound healing. 39,50 On the basis of these studies and our own findings, it is reasonable to speculate that high glucose-induced cutaneous O 2 Ϫ increase contributes, at least in part, to impaired NO function in diabetic wounds, and antioxidant strategies may be beneficial for improving diabetic wound healing.…”

Section: Oxidants and Antioxidants And Wound Healingmentioning

confidence: 99%

Gene Therapy of Endothelial Nitric Oxide Synthase and Manganese Superoxide Dismutase Restores Delayed Wound Healing in Type 1 Diabetic Mice

et al. 2004

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Background-Nitric oxide (NO) deficiency contributes to diabetic wound healing impairment. The present study tested the hypothesis that increased cutaneous superoxide (O 2 Ϫ ) levels in type 1 diabetic mice cause NO deficiency and delayed wound healing. Methods and Results-Wound healing was markedly delayed in streptozotocin-induced type 1 diabetic mice compared with the normal controls. There were significantly reduced levels of endothelial NO synthase (eNOS) protein and constitutive NOS activity in diabetic wounds, whereas O 2 Ϫ levels were markedly increased. A single regimen of cutaneous gene therapy of eNOS or manganese superoxide dismutase (MnSOD) restored such healing delay, with a concomitant suppression of wound O 2 Ϫ levels and augmentation of both eNOS protein and constitutive NOS activity. Gene therapy of MnSOD also increased cutaneous MnSOD activity. Cutaneous O 2 Ϫ levels were also increased in Ins2Akita diabetic mice. In vitro glucose treatment of cutaneous tissues from normal mice for 24 hours increased O 2

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Raxofelast, a hydrophilic vitamin E-like antioxidant, stimulates wound healing in genetically diabetic mice

Cited by 48 publications

References 26 publications

Cutaneous Wound Healing after Topical Application of <i>Pistacia atlantica</i> Gel Formulation in Rats

Cutaneous Wound Healing after Topical Application of <i>Pistacia atlantica</i> Gel Formulation in Rats

Abnormal angiogenesis in diabetes mellitus

Gene Therapy of Endothelial Nitric Oxide Synthase and Manganese Superoxide Dismutase Restores Delayed Wound Healing in Type 1 Diabetic Mice

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