This paper focuses on the adaptive neural output feedback control of a class of uncertain multi-input-multioutput nonlinear time-delay non-integer-order systems with unmeasured states, unknown control direction, and unknown asymmetric saturation actuator. Thus, the mean value theorem and a Gaussian error function-based continuous differentiable model are used in the paper to describe the unknown asymmetric saturation actuator and to get an affine model in which the control input appears in a linear fashion, respectively. The design of the controller follows a number of steps. Firstly, based on the semigroup property of fractional-order derivative, the system is transformed into a normalized fractional-order system by means of a state transformation in order to facilitate the control design. Then, a simple linear state observer is constructed to estimate the unmeasured states of the transformed system. A neural network is incorporated to approximate the unknown nonlinear functions while a Nussbaum function is used to deal with the unknown control direction. In addition, the strictly positive real condition, the Razumikhin Lemma, the frequency-distributed model, and the Lyapunov method are utilized to derive the parameter adaptive laws and to perform the stability proof. The main advantages of this work are that:(1) it can handle systems with constant, time-varying, and distributed time-varying delays, (2) the considered class of systems is relatively large, (3) the number of adjustable parameters is reduced, (4) the tracking errors converge asymptotically to zero and all signals of the closed-loop system are bounded. Finally, some simulation examples are provided to demonstrate the validity and effectiveness of the proposed scheme.