Sugarcane is a highly productive crop with high water requirements. In addition, its growth and sugar content are limited by the water deficit, a major problem affecting agriculture due to climate change. However, monitoring strategies are being developed worldwide that seek to increase productivity in the same area and with less water consumption. The sugarcane plants are produced sugar table, ethanol, and, from the hydrolysis of biomass, produce second-generation bioethanol, in addition to generating energy in thermoelectric plants. This research described the strategies and mechanisms used by sugarcane to tolerate water deficit. For this, a series of physiological, biochemical, enzymatic, morphological, anatomical, and ultrastructural analyses were developed. We used four commercial varieties of sugarcane, two tolerant and two sensitives, which were tested in all phases of the study. It was concluded that the variety RB92579 is drought tolerant as well as RB867515 and RB72454 genotype is sensitive to drought stress. Therefore, we proposed that variety RB855536 be assigned as an intermediary due to tolerance and sensitivity to water deficit.