RBOH-Dependent ROS Synthesis and ROS Scavenging by Plant Specialized Metabolites To Modulate Plant Development and Stress Responses

Chapman, Jordan M.; Mühlemann, Joëlle K.; Gayomba, Sheena R.; Muday, Gloria K.

doi:10.1021/acs.chemrestox.9b00028

Cited by 261 publications

(205 citation statements)

References 322 publications

(933 reference statements)

Supporting

Mentioning

195

Contrasting

Order By: Relevance

“…Cytosolic pH [25,26,39] and NOX enzymes [40][41][42][43][44] have both complex regulations and multiple physiological roles and we found a connection between these two important actors. Future studies should identify the mechanism of activation of NOXs by low pHc and the cellular targets regulating growth.…”

Section: Regulation Of Nadph Oxidases By Phmentioning

confidence: 54%

An Arabidopsis Mutant Over-Expressing Subtilase SBT4.13 Uncovers the Role of Oxidative Stress in the Inhibition of Growth by Intracellular Acidification

Bissoli

Muñoz‐Bertomeu

Bueso

et al. 2020

IJMS

View full text Add to dashboard Cite

Intracellular acid stress inhibits plant growth by unknown mechanisms and it occurs in acidic soils and as consequence of other stresses. In order to identify mechanisms of acid toxicity, we screened activation-tagging lines of Arabidopsis thaliana for tolerance to intracellular acidification induced by organic acids. A dominant mutant, sbt4.13-1D, was isolated twice and shown to over-express subtilase SBT4.13, a protease secreted into endoplasmic reticulum. Activity measurements and immuno-detection indicate that the mutant contains less plasma membrane H + -ATPase (PMA) than wild type, explaining the small size, electrical depolarization and decreased cytosolic pH of the mutant but not organic acid tolerance. Addition of acetic acid to wild-type plantlets induces production of ROS (Reactive Oxygen Species) measured by dichlorodihydrofluorescein diacetate. Acid-induced ROS production is greatly decreased in sbt4.13-1D and atrboh-D,F mutants. The latter is deficient in two major NADPH oxidases (NOXs) and is tolerant to organic acids. These results suggest that intracellular acidification activates NOXs and the resulting oxidative stress is important for inhibition of growth. The inhibition of acid-activated NOXs in the sbt4.13-1D mutant compensates inhibition of PMA to increase acid tolerance.

show abstract

Section: Regulation Of Nadph Oxidases By Phmentioning

confidence: 54%

An Arabidopsis Mutant Over-Expressing Subtilase SBT4.13 Uncovers the Role of Oxidative Stress in the Inhibition of Growth by Intracellular Acidification

Bissoli

Muñoz‐Bertomeu

Bueso

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…Among the other genes differentially regulated in roots in Lachit versus Hacha, RBOHG, RBOHB and the HMT/D, other OPT and MATE genes have not been sufficiently characterized in the context of metal homeostasis in rice. NADPH oxidase/respiratory burst oxidase homolog (RBOH) proteins generate localized ROS increases to regulate developmental and stress responses (Chapman, Muhlemann, Gayomba, & Muday, 2019), and down-regulation upon high Fe may prevent excessive ROS signaling. Phenylalanine ammonia lyase PAL02 and caffeoyl-CoA-O-methyltransferase ROMT16 (CCoAOMT-1) act in concert in the coumarin biosynthesis pathway.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic expression patterns of two contrasting lowland rice varieties reveal high iron stress tolerance

Kar

Khalouf

et al. 2020

Preprint

View full text Add to dashboard Cite

analyzed the data; P.B. and H.-J.M. wrote the original draft; and H.-J.M., A.B., S.K.P., G.X., L.S. and P.B. reviewed and edited the article; P.B. acquired funding. Summary statementLowland rice varieties Hacha and Lachit were selected for contrasting abilities to cope with iron excess stress. Morphological and physiological phenotypes were mirrored by molecular transcriptome changes, indicating altered metal homeostasis in the root as an adaptive tolerance mechanism in Lachit. AbstractIron (Fe) toxicity is a major challenge for plant cultivation in acidic water-logged soil environments, where lowland rice is a major staple food crop. Only few studies addressed the molecular characterization of excess Fe tolerance in rice, and these highlight different mechanisms for Fe tolerance in the studied varieties.Here, we screened 16 lowland rice varieties for excess Fe stress growth responses to identify contrasting lines, Fe-tolerant Lachit and -susceptible Hacha. Hacha and Lachit differed in their physiological and morphological responses to excess Fe, including leaf growth, leaf rolling, reactive oxygen species generation, Fe and metal contents. These responses were mirrored by differential gene expression patterns, obtained through RNA-sequencing, and corresponding GO term enrichment in tolerant versus susceptible lines. From the comparative transcriptomic profiles between Lachit and Hacha in response to excess Fe stress, individual genes of the category metal homeostasis, mainly root-expressed, may contribute to the tolerance of Lachit. 22 out of these 35 metal homeostasis genes are present in selection sweep genomic regions, in breeding signatures and/or differentiated during rice domestication. These findings will serve to design targeted Fe tolerance breeding of rice crops.

show abstract

“…Nonenzymatic ROS production can occur in mitochondria and chloroplast through electron transport chains (ETC) [ 61 , 62 , 63 ]. Enzymatic ROS production can occur in peroxisomes, cell walls, plasma membrane and apoplast [ 64 ], and also through respiratory burst oxidase homologs (RBOHs), a plasma-membrane-bound NADPH oxidase [ 62 , 65 ]. ROS induce [Ca 2+ ] cyt elevations by activation of the specialized Ca 2+ -permeable ion channels in the plasma membrane.…”

Section: Fruit Tree Responses To O 2 Deficiencymentioning

confidence: 99%

“…Elevation of [Ca 2+ ] cyt under hypoxia triggers multiple metabolic events and it is associated with both early and late responses to low oxygen conditions (deeply reviewed in [ 67 ]). ROS generated in response to abiotic stresses may be involved in various responses, acting as signaling molecules or triggering ROS-induced cell death [ 65 ]. Under hypoxic stress conditions, ROS can be generated due to an impairment of photosynthesis and aerobic respiration processes by inhibiting mETC [ 54 , 61 , 68 ].…”

Section: Fruit Tree Responses To O 2 Deficiencymentioning

confidence: 99%

Keep Calm and Survive: Adaptation Strategies to Energy Crisis in Fruit Trees under Root Hypoxia

Salvatierra¹,

Toro²,

Mateluna³

et al. 2020

Plants

View full text Add to dashboard Cite

Plants are permanently facing challenges imposed by the environment which, in the context of the current scenario of global climate change, implies a constant process of adaptation to survive and even, in the case of crops, at least maintain yield. O2 deficiency at the rhizosphere level, i.e., root hypoxia, is one of the factors with the greatest impact at whole-plant level. At cellular level, this O2 deficiency provokes a disturbance in the energy metabolism which has notable consequences on the yield of plant crops. In this sense, although several physiological studies describe processes involved in plant adaptation to root hypoxia in woody fruit trees, with emphasis on the negative impacts on photosynthetic rate, there are very few studies that include -omics strategies for specifically understanding these processes in the roots of such species. Through a de novo assembly approach, a comparative transcriptome study of waterlogged Prunus spp. genotypes contrasting in their tolerance to root hypoxia was revisited in order to gain a deeper insight into the reconfiguration of pivotal pathways involved in energy metabolism. This re-analysis describes the classically altered pathways seen in the roots of woody fruit trees under hypoxia, but also routes that link them to pathways involved with nitrogen assimilation and the maintenance of cytoplasmic pH and glycolytic flow. In addition, the effects of root hypoxia on the transcription of genes related to the mitochondrial oxidative phosphorylation system, responsible for providing adenosine triphosphate (ATP) to the cell, are discussed in terms of their roles in the energy balance, reactive oxygen species (ROS) metabolism and aerenchyma formation. This review compiles key findings that help to explain the trait of tolerance to root hypoxia in woody fruit species, giving special attention to their strategies for managing the energy crisis. Finally, research challenges addressing less-explored topics in recovery and stress memory in woody fruit trees are pointed out.

show abstract

RBOH-Dependent ROS Synthesis and ROS Scavenging by Plant Specialized Metabolites To Modulate Plant Development and Stress Responses

Cited by 261 publications

References 322 publications

An Arabidopsis Mutant Over-Expressing Subtilase SBT4.13 Uncovers the Role of Oxidative Stress in the Inhibition of Growth by Intracellular Acidification

An Arabidopsis Mutant Over-Expressing Subtilase SBT4.13 Uncovers the Role of Oxidative Stress in the Inhibition of Growth by Intracellular Acidification

Transcriptomic expression patterns of two contrasting lowland rice varieties reveal high iron stress tolerance

Keep Calm and Survive: Adaptation Strategies to Energy Crisis in Fruit Trees under Root Hypoxia

Contact Info

Product

Resources

About