RcbHLH59-RcPRs module enhances salinity stress tolerance by balancing Na+/K+ through callose deposition in rose (<i>Rosa chinensis</i>)

Su, Lin; Zhang, Yichang; Yu, Shuang; Geng, Lifang; Lin, Shujun; Ouyang, Lin; Jiang, Xinqiang

doi:10.1093/hr/uhac291

Cited by 10 publications

(11 citation statements)

References 72 publications

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“…RcMYB8 improved drought and salt tolerance by influencing ROS dynamic changes and proline accumulation. In addition, previous studies have shown a connection between RcMYB8 and RcPR5/1 (Su et al 2021 ), and RcPR5/1 was proved to enhance salinity resistance in rose (Su et al 2023 ). We next examined the relevant gene expression corresponding to ROS scavenging, proline synthase and pathogenesis-related-5 (PR5) in both RcMYB8 -silenced and RcMYB8 -overexpressing plants.…”

Section: Resultsmentioning

confidence: 92%

“…Under drought or salinity stresses, plants accumulate some physiological indicators [relative conductivity (REL), Chl content, chlorophyll fluorescence, callose accumulation] to improve their adaptability under these unfavorable conditions (James et al 2018 ; Tang et al 2019 ; Su et al 2023 ). Among them, REL is a vital indicator reflecting the severity of cellular membrane impairment (James et al 2018 ).…”

Section: Discussionmentioning

confidence: 99%

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RcMYB8 enhances salt and drought tolerance in rose (Rosa chinensis) by modulating RcPR5/1 and RcP5CS1

Zhang,

Yu,

Niu

et al. 2024

Mol Horticulture

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Plant Myeloblastosis (MYB) proteins function crucially roles upon variegated abiotic stresses. Nonetheless, their effects and mechanisms in rose (Rosa chinensis) are not fully clarified. In this study, we characterized the effects of rose RcMYB8 under salt and drought tolerances. For induction of the RcMYB8 expression, NaCl and drought stress treatment were adopted. Rose plants overexpressing RcMYB8 displayed enhanced tolerance to salinity and drought stress, while silencing RcMYB8 resulted in decreased tolerance, as evidenced by lowered intra-leaf electrolyte leakage and callose deposition, as well as photosynthetic sustainment under stressed conditions. Here, we further show that RcMYB8 binds similarly to the promoters of RcPR5/1 and RcP5C51 in vivo and in vitro. Inhibiting RcP5CS1 by virus-induced gene silencing led to decreased drought tolerance through the reactive oxygen species (ROS) homeostatic regulation. RcP5CS1-silenced plants showed an increase in ion leakage and reduce of proline content, together with the content of malondialdehyde (MDA) increased, lowered activities of Catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD). Our study highlights the transcriptional modulator role of RcMYB8 in drought and salinity tolerances, which bridges RcPR5/1 and RcP5CS1 by promoting ROS scavenging. Besides, it is probably applicable to the rose plant engineering for enhancing their abiotic stress tolerances. Graphical Abstract

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Section: Resultsmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

RcMYB8 enhances salt and drought tolerance in rose (Rosa chinensis) by modulating RcPR5/1 and RcP5CS1

Zhang,

Yu,

Niu

et al. 2024

Mol Horticulture

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“…Salt treatment of rose leaves was described previously [ 68 ]. Thirty JDG and 30 DMS rose cuttings with good rooting and uniform appearance were selected, and mature leaves of similar size were collected.…”

Section: Methodsmentioning

confidence: 99%

Multi-omics analysis reveals key regulatory defense pathways and genes involved in salt tolerance of rose plants

Ren,

Yang,

Jing

et al. 2024

Horticulture Research

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Salinity stress causes serious damage to crops worldwide, limiting plant production. However, the metabolic and molecular mechanisms underlying the response to salt stress in rose (Rosa spp.) remain poorly studied. We therefore performed a multi-omics investigation of Rosa hybrida cv. Jardin de Granville (JDG) and Rosa damascena Mill. (DMS) under salt stress to determine the mechanisms underlying rose adaptability to salinity stress. Salt treatment of both JDG and DMS led to the buildup of reactive oxygen species (H2O2). Palisade tissue was more severely damaged in DMS than in JDG, while the relative electrolyte permeability was lower and the soluble protein content was higher in JDG than in DMS. Metabolome profiling revealed significant alterations in phenolic acid, lipids, and flavonoid metabolite levels in JDG and DMS under salt stress. Proteome analysis identified enrichment of flavone and flavonol pathways in JDG under salt stress. RNA sequencing showed that salt stress influenced primary metabolism in DMS, whereas it substantially affected secondary metabolism in JDG. Integrating these datasets revealed that the phenylpropane pathway, especially the flavonoid pathway, is strongly enhanced in rose under salt stress. Consistent with this, weighted gene coexpression network analysis (WGCNA) identified the key regulatory gene chalcone synthase 1 (CHS1), which is important in the phenylpropane pathway. Moreover, luciferase assays indicated that the bHLH74 transcription factor binds to the CHS1 promoter to block its transcription. These results clarify the role of the phenylpropane pathway, especially flavonoid and flavonol metabolism, in the response to salt stress in rose.

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“…For silencing of RhTSPO , a 400-bp fragment containing a 200-bp 3′ UTR fragment was cloned into pTRV2 using the Xba I and Kpn I restriction sites. Silencing of RhETR3 and RhTSPO in rose plants by VIGS was performed as previously described [ 53 ].…”

Section: Methodsmentioning

confidence: 99%

TSPO-induced degradation of the ethylene receptor RhETR3 promotes salt tolerance in rose (Rosa hybrida)

Zhao,

Jing,

et al. 2024

Horticulture Research

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The gaseous plant hormone ethylene regulates plant development, growth, and responses to stress. In particular, ethylene affects tolerance to salinity; however, the underlying mechanisms of ethylene signaling and salt tolerance are not fully understood. Here, we demonstrate that salt stress induces the degradation of the ethylene receptor ETHYLENE RESPONSE 3 (RhETR3) in rose (Rosa hybrid). Furthermore, the TspO/MBR (Tryptophan-rich sensory protein/mitochondrial benzodiazepine receptor) domain-containing membrane protein RhTSPO interacted with RhETR3 to promote its degradation in response to salt stress. Salt tolerance is enhanced in RhETR3-silenced rose plants but decreased in RhTSPO-silenced plants. The improved salt tolerance of RhETR3-silenced rose plants is partly due to the increased expression of ACC SYNTHASE1 (ACS1) and ACS2, which results in an increase in ethylene production, leading to the activation of ETHYLENE RESPONSE FACTOR98 (RhERF98) expression and, ultimately accelerating H2O2 scavenging under salinity conditions. Additionally, overexpression of RhETR3 increased the salt sensitivity of rose plants. Co-overexpression with RhTSPO alleviated this sensitivity. Together, our findings suggest that RhETR3 degradation is a key intersection hub for the ethylene signalling-mediated regulation of salt stress.

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RcbHLH59-RcPRs module enhances salinity stress tolerance by balancing Na+/K+ through callose deposition in rose (Rosa chinensis)

Cited by 10 publications

References 72 publications

RcMYB8 enhances salt and drought tolerance in rose (Rosa chinensis) by modulating RcPR5/1 and RcP5CS1

RcMYB8 enhances salt and drought tolerance in rose (Rosa chinensis) by modulating RcPR5/1 and RcP5CS1

Multi-omics analysis reveals key regulatory defense pathways and genes involved in salt tolerance of rose plants

TSPO-induced degradation of the ethylene receptor RhETR3 promotes salt tolerance in rose (Rosa hybrida)

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