RCC1 regulates inner centromeric composition in a Ran-independent fashion

Zhang, Michael Shaofei; Furuta, Maiko; Arnaoutov, Alexei; Dasso, Mary

doi:10.1080/15384101.2018.1442630

Cited by 8 publications

(6 citation statements)

References 24 publications

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“…Pim1 is the yeast homolog of the metazoan RCC1. Given that RCC1 is implicated in regulating centromeric composition in vitro (Zhang et al, 2018), we examined the association of Pim1 with the centromeric regions of wild‐type and nup132 Δ meiotic cells. Using ChIP‐qPCR, Pim1 was detected at centromeric loci, and its localization at centromeres was significantly reduced in the absence of Nup132 (Figure 7a).…”

Section: Resultsmentioning

confidence: 99%

“…Bub1 is precociously loaded onto the meiotic centromeres in the nup132 Δ meiotic cells (Yang et al, 2015), suggesting that Nup132 regulates kinetochore dynamics via Top2 SUMOylation. In addition, studies using Xenopus egg extract indicate that increasing the level of chromosome‐associated RCC1 reduces the degree of Bub1 occupancy at the centromeres (Arnaoutov & Dasso, 2003; Zhang et al, 2018). It is possible that the reduced chromosomal binding of Pim1 allows for the untimely localization of Bub1 at the centromeres in the nup132 Δ mutant.…”

Section: Discussionmentioning

confidence: 99%

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A nuclear pore complex‐associated regulation of SUMOylation in meiosis

Yang

Asakawa

et al. 2022

Genes to Cells

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The nuclear pore complex (NPC) provides a permeable barrier between the nucleoplasm and cytoplasm. In a subset of NPC constituents that regulate meiosis in the fission yeast Schizosaccharomyces pombe, we found that nucleoporin Nup132 (homolog of human Nup133) deficiency resulted in transient leakage of nuclear proteins during meiosis I, as observed in the nup132 genedeleted mutant. The nuclear protein leakage accompanied the liberation of the small ubiquitin-like modifier (SUMO)-specific ubiquitin-like protease 1 (Ulp1) from the NPC. Ulp1 retention at the nuclear pore prevented nuclear protein leakage and restored normal meiosis in a mutant lacking Nup132. Furthermore, using mass spectrometry analysis, we identified DNA topoisomerase 2 (Top2) and RCC1-related protein (Pim1) as the target proteins for SUMOylation. SUMOylation levels of Top2 and Pim1 were altered in meiotic cells lacking Nup132. HyperSUMOylated Top2 increased the binding affinity at the centromeres of nup132 gene-deleted meiotic cells. The Top2-12KR sumoylation mutant was less localized to the centromeric regions. Our results suggest that SUMOylation of chromatin-binding proteins is regulated by the NPCbound SUMO-specific protease and is important for the progression of meiosis.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A nuclear pore complex‐associated regulation of SUMOylation in meiosis

Yang

Asakawa

et al. 2022

Genes to Cells

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“…The two enzymes are involved in protein metabolization like proteasomal degradation and cell apoptosis, which are tightly related to inflammation, organ injury, virus infection, and tumors, etc. [ 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

Furosine Induced Apoptosis by the Regulation of STAT1/STAT2 and UBA7/UBE2L6 Genes in HepG2 Cells

Xing

Zhao

et al. 2018

IJMS

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As a typical product in the Miallard reaction, research on the quantitative detection of furosine is abundant, while its bioactivities and toxic effects are still unclear. Our own work recently demonstrated the induction of furosine on apoptosis in HepG2 cells, while the related mechanism remained elusive. In this study, the effects of furosine on cell viability and apoptosis were detected to select the proper dosage, and transcriptomics detection and data analysis were performed to screen out the special genes. Additionally, SiRNA fragments of the selected genes were designed and transfected into HepG2 cells to validate the role of these genes in inducing apoptosis. Results showed that furosine inhibited cell viability and induced cell apoptosis in a dose-dependent manner, as well as activated expressions of the selected genes STAT1 (signal transducer and activator of transcription 1), STAT2 (signal transducer and activator of transcription 2), UBA7 (ubiquitin-like modifier activating enzyme 7), and UBE2L6 (ubiquitin-conjugating enzyme E2L6), which significantly affected downstream apoptosis factors Caspase-3 (cysteinyl aspartate specific proteinase-3), Bcl-2 (B-cell lymphoma gene-2), Bax (BCL2-Associated gene X), and Caspase-9 (cysteinyl aspartate specific proteinase-9). For the first time, we revealed furosine induced apoptosis through two transcriptional regulators (STAT1 and STAT2) and two ubiquitination-related enzymes (UBA7 and UBE2L6).

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“…While these pathways seem to be distinct [Sampath et al., 2004; Maresca et al., 2009], relationships exist between the two. For instance, RCC1 acts at the kinetochore to modulate the spindle assembly checkpoint [Arnaoutov and Dasso, 2003], where it regulates the metaphase‐anaphase transition through the displacement of Sgo1 and CPC [Zhang et al., 2018]. In addition, the stabilisation of k‐fibre minus‐ends is ensured by a Ran‐GTP‐dependent mechanism [Meunier and Vernos, 2011] and Ran‐GTP is crucial for the maintenance of stable kinetochore‐MT attachments through the regulation of Aurora B [Lee et al., 2012].…”

Section: Mechanisms Of Spindle Mt Nucleationmentioning

confidence: 99%

Mechanisms of spindle assembly and size control

Gibeaux

2020

Biology of the Cell

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The spindle is crucial for cell division by allowing the faithful segregation of replicated chromosomes to daughter cells. Proper segregation is ensured only if microtubules (MTs) and hundreds of other associated factors interact to assemble this complex structure with the appropriate architecture and size. In this review, we describe the latest view of spindle organisation as well as the molecular gradients and mechanisms underlying MT nucleation and spindle assembly. We then discuss the overlapping physical and molecular constraints that dictate spindle morphology, concluding with a focus on spindle size regulation.

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RCC1 regulates inner centromeric composition in a Ran-independent fashion

Cited by 8 publications

References 24 publications

A nuclear pore complex‐associated regulation of SUMOylation in meiosis

A nuclear pore complex‐associated regulation of SUMOylation in meiosis

Furosine Induced Apoptosis by the Regulation of STAT1/STAT2 and UBA7/UBE2L6 Genes in HepG2 Cells

Mechanisms of spindle assembly and size control

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