Rce1 suppresses invasion and metastasis of hepatocellular carcinoma via epithelial‐mesenchymal transition induced by the TGF‐β1/H‐Ras signaling pathway

Ma, Chaoqun; Yang, Yan; Xu, Lei; Tu, Wei; Chen, Feng; Wang, Jianming

doi:10.1002/jcp.29155

Cited by 12 publications

(9 citation statements)

References 36 publications

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“…Oppositely, several studies have also reported its suppressive functions in HCC, which hinges on the intracellular p53 status (37,38). HRAS, a member of the RAS gene family that is closely related to multiple human cancers, and studies have shown that it engages in mediating EMT of HCC cells through the TGF-β1/HRAS axis, thereby promoting tumor invasion and metastasis (39). And the expression of GNA14 is decreased in tumor tissues and is related to the poor OS of patients with HCC, indicating that it can be a tumor suppressor gene.…”

Section: Discussionmentioning

confidence: 99%

Genetic Analysis of Platelet-related Genes in Hepatocellular Carcinoma Reveals a Novel Prognostic Signature and Determines PRKCD as the Potential Molecular Bridge

Zhao

et al. 2022

Preprint

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Background Hepatocellular carcinoma (HCC) belongs to a representative lethality gastrointestinal malignancy, and comprehensive management of HCC remains intractable at present on account of its invasive biological feature that is easy to relapse and early metastasis. The intimate connection between platelets and tumor progression has been widely reported, and platelet-related indicators are also used in the clinical practice of carcinoma. This work is designed to investigate the significance of platelet-related genes in the prognostic prediction of patients with HCC and their potential role in the cross-talk between HCC cells and platelets in the tumor microenvironment. Methods By integrating the RNA-seq data and clinicopathological information of HCC patients, we extracted prognosis-associated platelet-related genes based on the univariate cox analysis and further established a relevant prognostic signature via the lasso cox regression analysis, and two independent HCC cohorts were used as external validation. Multiple bioinformatics methods were utilized to explore the underlying functional discrepancy between different risk groups classified by the risk model. And in vitro proliferation, invasion, and migration assays were conducted to investigate the effect of platelet stimulation on HCC cells’ viability and motility, and flow cytometric analysis was exerted to demonstrate the influence of HCC cells on platelet activation. Results A novel platelet-related risk model was developed and patients both in the training and testing cohorts were divided into distinct risk subgroups according to the median risk score. It was observed that the high-risk status was closely associated with poor prognosis and worse clinicopathological parameters. Meanwhile, an obvious discrepancy in the constitution of the immune microenvironment also indicated that distinct immune status might be a potential determinant affecting prognosis as well as immunotherapy reactiveness. Moreover, in vitro experiments demonstrated that PRKCD could act as a molecular bridge between tumor cells and platelets, which could either participate in regulating tumor malignant phenotype or mediating platelet activation. Conclusions In brief, this work reveals a novel platelet-related risk signature for prognostic evaluation of HCC patients and confirms that PRKCD is a key messenger in HCC cell-platelet interaction and plays a crucial role in mediating platelet-induced tumor progression.

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Section: Discussionmentioning

confidence: 99%

Genetic Analysis of Platelet-related Genes in Hepatocellular Carcinoma Reveals a Novel Prognostic Signature and Determines PRKCD as the Potential Molecular Bridge

Zhao

et al. 2022

Preprint

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“…The expression of PIK3R1 in HCC tissues was higher than that in adjacent normal tissues, and knockdown of PIK3R1 could suppress the proliferation and migration of HCC cells [ 51 ]. Besides, HRAS gene was associated with the early invasion and metastasis of HCC caused by epithelial-mesenchymal transition [ 52 ]. Therefore, previous studies made our results more plausible.…”

Section: Discussionmentioning

confidence: 99%

Determining the Traditional Chinese Medicine (TCM) Syndrome with the Best Prognosis of HBV-Related HCC and Exploring the Related Mechanism Using Network Pharmacology

Wei

Wang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Background. In traditional Chinese medicine (TCM), TCM syndrome is a key guideline, and Chinese materia medicas are widely used to treat hepatitis B virus- (HBV-) related hepatocellular carcinoma (HCC) according to different TCM syndromes. However, the prognostic value of TCM syndromes in HBV-related HCC patients has never been studied. Methods. A retrospective cohort of HBV-related HCC patients at Shenzhen Traditional Chinese Medicine Hospital from December 2005 to October 2017 was analyzed. The prognostic value of TCM syndromes in HBV-related HCC patients was assessed by Kaplan–Meier survival curves and Cox analysis, and the TCM syndrome with the best prognosis of HBV-related HCC patients was determined. To further study the relevant mechanisms, key Chinese materia medicas (KCMMs) for the TCM syndrome with the best prognosis were summarized, and network pharmacology was also performed. Results. A total of 207 HBV-related HCC patients were included in this research, and we found that HBV-related HCC patients with TCM excess syndrome had better OS. Then, a total of eight KCMMs for TCM excess syndrome were identified, whose crucial ingredients included quercetin, beta-sitosterol, kaempferol, luteolin, and XH-14, and KCMMs could play a therapeutic role through MAPK, JAK-STAT, Wnt, Hippo, and other pathways. Moreover, TP53, SRC, STAT3, MAPK3, PIK3R1, HRAS, VEGFA, HSP90AA1, EGFR, and JAK2 were determined as the key targets. Conclusion. We propose a new research method of “prognosis of TCM syndromes-KCMMs-network pharmacology” to reveal the prognostic value of TCM syndromes and the potential mechanism by which TCM syndromes affect prognosis.

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“…Studies have reported that EMT is the driving force for cancer cells metastasis, the EMT is an important mechanism in the early stage of cancer metastasis, to study the molecular mechanism of EMT is the key to improve tumor diagnosis and treatment. [26,27] The decreased expression of E-cadherin and Vimentin, N-cadherin and other proteins have increased which have been considered to be the most signi cant feature of EMT. The changes of these EMT markers have enabled cancer cells to obtain features that promote migration and invasion.…”

Section: Discussionmentioning

confidence: 99%

CircPVT1 Promotes Bladder Cancer Progression by Acting as a ceRNA for miR-140-3p to Target TRPS1

Chi¹,

Sun²,

Zhao³

et al. 2020

Preprint

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Background: Bladder cancer (BC) is one of the most malignancy tumor in the urinary system. Therefore, further studies are needed to revealed the molecular mechanism of BC progression and development. Previous study demonstrated that the deregulation of circRNAs can regulate cell biological functions in tumorigenesis and development. However, the roles of circPVT1 in BC have not yet been revealed. Materials and methods: The expression level of circPVT1, miR-140-3p, and TRPS1 were measured by RT-PCR in BC tissues and cells; dual luciferase reporter and RIP assay showed that circRNA served as a sponge for miRNA, and miRNA could target mRNA. In vitro, effects of overexpression circPVT1 or sh-circPVT1 can regulate BC cells’ proliferation, migration, invasion were detected by CCK-8 assay, wound healing assay, and transwell assay. Results: Our research demonstrated that the expression of circPVT1 was upregulated in BC tissues and cell lines, and increased in metastatic tissues compared to that of non-metastatic tissues. CircPVT1 sponging miR-140-3p to target TRPS1 was revealed by dual luciferase reporter and RIP assay. In addition, the expression level of miR-140-3p was reduced in BC tumor tissues, and TRPS1 was significantly increased in BC tumor tissues. Pearson correlation analysis showed that miR-140-3p with circPVT1 and TRPS1 as compare with miR-140-3p have a moderately negative correlation, and there was a moderately positive correlation between circPVT1 with TRPS1. Further, cytological studies found that circPVT1 enhance BC cells’ proliferation, migration, and invasion by targeting TRPS1 via miR-140-3p. Conclusion: CircPVT1 plays a tumor enhancement role in BC and that can effectively promote cell proliferation, migration, invasion and EMT by targeting the miR-140-3p/TRPS1 axis. CircPVT1 may be a novel potential treatment and diagnosis biomarker in BC.

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Rce1 suppresses invasion and metastasis of hepatocellular carcinoma via epithelial‐mesenchymal transition induced by the TGF‐β1/H‐Ras signaling pathway

Cited by 12 publications

References 36 publications

Genetic Analysis of Platelet-related Genes in Hepatocellular Carcinoma Reveals a Novel Prognostic Signature and Determines PRKCD as the Potential Molecular Bridge

Genetic Analysis of Platelet-related Genes in Hepatocellular Carcinoma Reveals a Novel Prognostic Signature and Determines PRKCD as the Potential Molecular Bridge

Determining the Traditional Chinese Medicine (TCM) Syndrome with the Best Prognosis of HBV-Related HCC and Exploring the Related Mechanism Using Network Pharmacology

CircPVT1 Promotes Bladder Cancer Progression by Acting as a ceRNA for miR-140-3p to Target TRPS1

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Rce1 suppresses invasion and metastasis of hepatocellular carcinoma via epithelial‐mesenchymal transition induced by the TGF‐β1/H‐Ras signaling pathway

Cited by 12 publications

References 36 publications

Genetic Analysis of Platelet-related Genes in Hepatocellular Carcinoma Reveals a Novel Prognostic Signature and Determines PRKCD as the Potential Molecular Bridge

Genetic Analysis of Platelet-related Genes in Hepatocellular Carcinoma Reveals a Novel Prognostic Signature and Determines PRKCD as the Potential Molecular Bridge

Determining the Traditional Chinese Medicine (TCM) Syndrome with the Best Prognosis of HBV-Related HCC and Exploring the Related Mechanism Using Network Pharmacology

CircPVT1 Promotes Bladder Cancer&nbsp;Progression by Acting as a ceRNA for miR-140-3p&nbsp;to Target TRPS1

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CircPVT1 Promotes Bladder Cancer Progression by Acting as a ceRNA for miR-140-3p to Target TRPS1