Re‐Epithelialization of Wounds

Larjava, Hannu; and, Lari Häkkinen; Koivisto, Leeni

doi:10.1002/9781118704509.ch5

Cited by 5 publications

(8 citation statements)

References 360 publications

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“…This result suggests that implanted keratinocytes and fibroblasts play a role in re‐epithelialization, a process, which involves the migration, proliferation, and differentiation of keratinocytes . It has been extensively reported that this process is regulated by cytokines and growth factors such as transforming growth factor released by keratinocytes, and keratinocyte growth factor, and epidermal growth factor released by fibroblasts . Our results also showed differences between the thickness of the regenerated dermal layer in different groups.…”

Section: Discussionsupporting

confidence: 69%

“…38 It has been extensively reported that this process is regulated by cytokines and growth factors such as transforming growth factor released by keratinocytes, and keratinocyte growth factor, and epidermal growth factor released by fibroblasts. [39][40][41] Our results also showed differences between the thickness of the regenerated dermal layer in different groups. In particular, it was observed that the dermis layers in wounds treated with ibuprofen scaffolds were thinner than controls.…”

Section: Discussionsupporting

confidence: 62%

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Ibuprofen loaded PLA nanofibrous scaffolds increase proliferation of human skin cells in vitro and promote healing of full thickness incision wounds in vivo

et al. 2015

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This article presents successful incorporation of ibuprofen in polylactic acid (PLA) nanofibers to create scaffolds for the treatment of both acute and chronic wounds. Nanofibrous PLA scaffolds containing 10, 20, or 30 wt % ibuprofen were created and ibuprofen release profiles quantified. In vitro cytotoxicity to human epidermal keratinocytes (HEK) and human dermal fibroblasts (HDF) of the three scaffolds with varying ibuprofen concentrations were evaluated and compared to pure PLA nanofibrous scaffolds. Thereafter, scaffolds loaded with ibuprofen at the concentration that promoted human skin cell viability and proliferation (20 wt %) were evaluated in vivo in nude mice using a full thickness skin incision model to determine the ability of these scaffolds to promote skin regeneration and/or assist with scarless healing. Both acellular and HEK and HDF cell-seeded 20 wt % ibuprofen loaded nanofibrous bandages reduced wound contraction compared with wounds treated with Tegaderm™ and sterile gauze. Newly regenerated skin on wounds treated with cell-seeded 20 wt % ibuprofen bandages exhibited significantly greater blood vessel formation relative to acellular ibuprofen bandages. We have found that degradable anti-inflammatory scaffolds containing 20 wt % ibuprofen promote human skin cell viability and proliferation in vitro, reduce wound contraction in vivo, and when seeded with skin cells, also enhance new blood vessel formation. The approaches and results reported here hold promise for multiple skin tissue engineering and wound healing applications. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 327-339, 2017.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionsupporting

confidence: 62%

Ibuprofen loaded PLA nanofibrous scaffolds increase proliferation of human skin cells in vitro and promote healing of full thickness incision wounds in vivo

et al. 2015

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“…The epidermis is the outer layer of the skin and creates an important barrier, which protects from physical, chemical, and microbial penetration and damage (Larjava, Häkkinen, & Koivisto, 2012). Epithelialization is the process in which keratinocytes migrate from the lower layers of skin and divide (Panchatcharam et al, 2006).…”

Section: Effects Of Curcumin On Re-epithelialization and Remodelingmentioning

confidence: 99%

Curcumin nanofibers for the purpose of wound healing

Fereydouni

Darroudi

Movaffagh

et al. 2018

Journal Cellular Physiology

129

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Poor wound healing is a highly prevalent clinical problem with, as yet, no entirely satisfactory solution. A new technique, termed electrospinning, may provide a solution to improve wound healing. Due to their large surface area to volume ratio and porosity, the nanofibers created by electrospinning are able to deliver sustained drug release and oxygen to the wound. Using different types of polymers with varying properties helps strengthening nanofiber and exudates absorption. The nanofibers appear to have an ideal structure applicable for wound healing and, in combination with curcumin, can blend the anti-inflammatory and antioxidant properties of curcumin into a highly effective wound dressing. The use of suitable curcumin solvents and the slow release of curcumin from the nanofiber help in overcoming the known limitations of curcumin, specifically its low stability and limited bioavailability.Here, we review the studies which have been done on synthesized nanofibers containing curcumin, produced by the electrospinning technique, for the purpose of wound healing.

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“…The presence of the clot and contraction could explain the mouse's larger epithelial front. A larger mass of cells permits the production of more enzymes needed to degrade the damaged contracted tissue and clot [Larjava et al, 2012]. The axolotl's liquid environments may alter the blood clot's formation contrasting open-air wounds.…”

Section: Discussion/conclusionmentioning

confidence: 99%

“…This exploratory study overviews the relatively early phases of wound healing including: haemorrhage/hemostasis [Oakley and Larjava, 2012], epithelial migration [Larjava et al, 2012;Huang et al, 2017], and the inflammatory response [Turabelidze and DiPietro, 2012;Seifert and Maden, 2014] after a similar wound in both animals' tongues and intermandibular regions. We expected the wounds to create observable and quantifiable data highlighting certain important differences in these animals early wound responses.…”

Section: Introductionmentioning

confidence: 99%

Axolotls’ and Mices’ Oral-Maxillofacial Trephining Wounds Heal Differently

Charbonneau

Åström

Salo

et al. 2021

Cells Tissues Organs

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The <i>Ambystoma maxicanum</i> (axolotl) regenerates strikingly from wounds and amputations. Comparing its healing ability to non-regenerative species such as the mouse should help narrow in on mechanisms to improve human wound healing. Here, the tongue and intermandibular soft tissues of both mice (C57BL/6NCrl) and axolotls were wounded with a 2–2.5 mm punch biopsy. The study aimed to compare the differences between these 2 species following surgical resection with regard to the macroscopic and histological characteristics. These include wound closure times, epithelial wound sealing and thickness as well as acute immune marker myeloperoxidase (MPO) response over 30 days. Post surgery, mice visually showed greater haemorrhage; their wounds immediately collapsed while it took 14 days for the axolotls mandibular void to close. The epithelium sealed the axolotls’ wound margins within 24 h with a maximal mean thickness of 0.42 ± 0.13-fold normalized to unwounded skin. In mice, the epithelium separately sealed the ventral and dorsal sides, respectively at 7 and 7–30 days with mean maximal epithelial thicknesses reaching 13 ± 5.6 and 3.0 ± 0.63-fold. Mean MPO-positive cell values peaked in axolotls at 14 ± 1.5-fold between hours 6–12; while in mice, it peaked at 8.7 ± 0.9-fold between hours 24–96. We conclude that axolotls form smaller blood clots, have a faster and thinner epithelial cell migrating front, and a shorter MPO-positive cell response in comparison to mice. These observations may help refine future oral and facial wound-healing research and treatment.

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Re‐Epithelialization of Wounds

Cited by 5 publications

References 360 publications

Ibuprofen loaded PLA nanofibrous scaffolds increase proliferation of human skin cells in vitro and promote healing of full thickness incision wounds in vivo

Ibuprofen loaded PLA nanofibrous scaffolds increase proliferation of human skin cells in vitro and promote healing of full thickness incision wounds in vivo

Curcumin nanofibers for the purpose of wound healing

Axolotls’ and Mices’ Oral-Maxillofacial Trephining Wounds Heal Differently

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