This paper assesses long-term trends in human activity across the Iberian Peninsula associated with the spread of agriculture. In order to improve our understanding of regional trajectories within the two-way and multiple-velocity model proposed by Isern et al. (Journal of Archaeological Method and Theory 21:447-460, 2014), we consider summed calibrated radiocarbon dates of a large audited dataset from across Iberia. To assess the role of different analytical units, two alternative peninsular divisions at different spatial resolutions were used: one, with three generalized study areas, and the second, with eight biogeographic regions. This comparison of spatiotemporal variability provides a more in-depth inter-regional reading of the Neolithic transition in Iberia than more generalized approaches. The southwest, Mediterranean coasts and interior portions of Iberia all showed significant demographic expansion with the onset of the Neolithic, as observed across Western Europe. By contrast, northern Iberia featured a close adherence to a null model of exponential growth and a longer continuation of hunter-gatherer strategies.