Re-evaluation of saliva for monitoring theophylline concentrations.

Rylance, G; Beswick, D T; Cullen, R E; Roberts, Dawn

doi:10.1136/adc.60.1.66

Cited by 10 publications

(2 citation statements)

References 8 publications

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“…Monitoring of serum theophylline levels is well established because of the narrow range of optimal serum concentration and the great interindividual differences in pharmacokinetics especially in ~h i l d r e n .~.~ Knowledge of theophylline levels is also useful in assessing and improving patient ~o m p l i a n c e .~ In order to make theophylline drug monitoring more convenient for the patient, numerous investigators have studied the accuracy of salivary theophylline levels in predicting serum levels and have obtained conflicting results . [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] In this study we have investigated the accuracy and predictive value of saliva theophylline measurements using fluorescence polarization immunoassay (FPIA), which has been proven to be a reliable The Seralyzer (reagent strip reflectance photometer method), which can be used for rapid theophylline determination in an ambulatory setting,21,22 was simultaneously tested. We tried to make sampling of saliva easier by using a special device.…”

Section: Introductionmentioning

confidence: 99%

Monitoring theophylline levels in serum and saliva using fluorescence polarization immunoassay and seralyzer

Schulte¹,

W²,

Schmidt³

et al. 1989

Pediatric Pulmonology

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The reliability and predictive value of salivary theophylline levels was investigated in 58 children who were treated with oral sustained-release theophylline preparations for bronchial asthma. Serum and saliva levels were assessed by fluorescence polarization immunoassay (FPIA), and Seralyzer (reagent strip reflectance photometer method). Forty-six paired serum and saliva samples from 28 patients (Group 1) were used to develop a linear regression formula. This formula was tested on 130 paired serum and saliva samples from 32 patients (Group 2). When saliva theophylline levels were assayed using FPIA, 95% of serum levels were predicted within 30% of the measured value. Using Seralyzer, 95% of serum levels were predicted within 45% of the measured value. For patients with three and more paired serum and saliva specimens, individual regression formulas were determined showing considerable variation. This study indicates that salivary theophylline levels provide for an estimation of serum levels if FPIA is used. The Seralyzer technique is only useful for serum theophylline measurements. Using a special device it is now easier to obtain saliva samples than by conventional methods.

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Section: Introductionmentioning

confidence: 99%

Monitoring theophylline levels in serum and saliva using fluorescence polarization immunoassay and seralyzer

Schulte¹,

W²,

Schmidt³

et al. 1989

Pediatric Pulmonology

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“…2 Theophylline concentrations measured in saliva have been shown to be unreliable. 3 Various techniques are used to measure theophylline concentrations in serum. The purpose of this study was to compare results obtained by high performance liquid chromatography (HPLC) with those of a simple stick assay carried out at the bedside.…”

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confidence: 99%

Whole blood assay of theophylline concentrations using immunochromatographic stick at the bedside.

Elias-Jones

Cottle

Leakey

et al. 1987

Archives of Disease in Childhood

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Serum/Saliva Correlations for Theophylline in Asthmatics

Blanchard¹,

Harvey²,

Morgan³

1991

The Journal of Clinical Pharma

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Theophylline levels in mixed saliva (both stimulated and unstimulated) were compared with total and free (unbound) serum theophylline levels in 28 asthmatic outpatients using theophylline regularly. Stimulated saliva predicted both total and unbound serum theophylline concentrations within +/- 1 microgram/mL in 62.5% and 92.9%, respectively, of the samples examined. In addition, the total serum levels could be used to predict unbound serum concentrations to within +/- 1 mg/L in 100% of the cases that were examined. These results indicate that saliva levels predict the unbound serum theophylline levels with greater accuracy and precision than they predict total serum theophylline levels. In addition, total serum levels can be used to reliably predict unbound serum levels. The use of mixed stimulated saliva is recommended as a reliable non-invasive method for monitoring unbound serum theophylline levels. The therapeutic range for saliva, which corresponds to the accepted total serum concentration range of 10-20 mg/L, is approximately 5.6-11.3 mg/L.

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Re-evaluation of saliva for monitoring theophylline concentrations.

Abstract: 6 Manciaux M. 'Some monographs' in children and accidents. Children in the tropics 1980;123:6-10. 7 Mero Asagba GEE. Three villages' memory of accidents involving children in thrcc gencrations.

Cited by 10 publications

References 8 publications

Monitoring theophylline levels in serum and saliva using fluorescence polarization immunoassay and seralyzer

Monitoring theophylline levels in serum and saliva using fluorescence polarization immunoassay and seralyzer

Whole blood assay of theophylline concentrations using immunochromatographic stick at the bedside.

Serum/Saliva Correlations for Theophylline in Asthmatics

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