“…After 150 s (time point t 3 , 250 s), 10 mL of diaphorase (Worthington Biochemical Corporation, Lakewood, NJ) 10 mg/mL solution was added. The final step involved the addition of 0.62 mL of a 10 mM stock solution of dialkylated diaza lariat ether (LEC 10 ) (16,20,24) in TFE (time point t 4 , 400 s), for a final LEC 10 concentration of 2 mM; or 3.1 mL of a 2 mM stock solution of valinomycin (Sigma) in dimethyl sulfoxide, for a final valinomycin concentration of 2 mM; or 3.1 mL of a 20 mg/mL stock solution of lysozyme in lactate/NAD þ solution, for a final lysozyme concentration of 20 mg/mL; or 3.1 mL of 10 mM stock solution of each of the MABCE compounds in TFE, for final concentrations of 10 mM. Fluorescence intensity FIGURE 1 Synthesis of monoacylated and monoalkylated benzo crown-ether compounds.…”