Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill

Development of an in vitro-based potency assay for anthrax vaccine

“…After incubating for 4 h at 37 • C, 5% CO 2 , 25 l of 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide (MTT, Sigma) at 5 mg/ml of PBS was added to each well. After incubating at 37 • C, 5% CO 2 for 2 h, the cells were lysed, and the precipitate was dissolved by adding 100 l per well of solubilization buffer which consisted of 10% SDS in 50% dimethylformamide, pH 4.8 [25]. Absorbance readings were obtained using a BioTek 312e microplate reader (BioTek) at a wavelength of 570 nm less the reference wavelength at 690 nm after an overnight incubation at 37 • C. Each dilution of sample and standard was tested in triplicate.…”

The PTEN/MMAC1/TEP tumor suppressor gene decreases cell growth and induces apoptosis and anoikis in breast cancer cells

“…Cell growth was assessed by MTT (3,(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide) dye conversion at 570 nm as described (Hansen et al, 1989). Brie¯y, cells were seeded in 96-well plates (¯at bottom, 5000 cells/well) and infected with adenovirus containing wild-type PTEN or empty virus (DE1).…”

Treatment of human breast cancer cells with antisense RNA to the type I insulin-like growth factor receptor inhibits cell growth, suppresses tumorigenesis, alters the metastatic potential, and prolongs survival in vivo

“…26 Therefore, a modified MTT assay was used to determine cell proliferation rates in MDA-MB-435s human breast cancer cells from clones C8 and C9 carrying antisense IGF-IR, cells transfected with sense IGF-IR, or cells transfected with the control vector. Cells were seeded into each well (2.5 ϫ 10 3 cells/well) of 96-well plates.…”