Re-introduction of an extinct population of Pulsatilla patens using different propagation techniques

Żabicka, Justyna; Żabicki, Piotr; Słomka, Aneta; Śliwińska, Elwira; Jędrzejczyk-Korycińska, Monika; Nowak, Teresa; Migdałek, Grzegorz; Kwiatkowska, Monika; Kuta, Elżbieta

doi:10.1038/s41598-022-18397-0

Cited by 2 publications

(1 citation statement)

References 42 publications

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“…These studies also aimed to find whether mitochondrial genomes, the same as chloroplast genomes, could prove to be useful tools in the analysis of populations genomics and phylogeography of plants. Molecular identification of populations and individuals is especially important for reintroduction programs, enabling proper selection of transplant genotypes for specific restitution areas, and maintaining natural genetic differentiation among populations [22].…”

Section: Introductionmentioning

confidence: 99%

Are the Organellar Genomes Useful for Fine Scale Population Structure Analysis of Endangered Plants?—A Case Study of Pulsatilla patens (L.) Mill

Szandar

Sawicki

Paukszto

et al. 2022

Genes

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Pulsatilla patens is a rare and endangered species in Europe and its population resources have significantly decreased over the past decades. Previous genetic studies of this species made it possible to estimate the genetic diversity of the European population and to describe the structure of chloroplast and mitochondrial genomes. The main aim of these studies was to characterize the variability of chloroplast and mitochondrial genomes in more detail at the intra-population and inter-population levels. Our study presents new organelle genome reference sequences that allow the design of novel markers that can be the starting point for testing hypotheses, past and modern biogeography of rare and endangered species P. patens, and adaptive responses of this species to changing environments. The study included sixteen individuals from five populations located in Northeastern Poland. Comparative analysis of 16 P. patens plastomes from 5 populations enabled us to identify 160 point mutations, including 64 substitutions and 96 InDels. The most numerous detected SNPs and Indels (75%) were accumulated in three intergenic spacers: ndhD—ccsA, rps4—rps16, and trnL(UAG)—ndhF. The mitogenome dataset, which was more than twice as large as the plastome (331 kbp vs. 151 kbp), revealed eight times fewer SNPs (8 vs. 64) and six times fewer InDels (16 vs. 96). Both chloroplast and mitochondrial genome identified the same number of haplotypes—11 out of 16 individuals, but both organellar genomes slightly differ in haplotype clustering. Despite the much lower variation, mitogenomic data provide additional resolution in the haplotype detection of P. patens, enabling molecular identification of individuals, which were unrecognizable based on the plastome dataset.

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Section: Introductionmentioning

confidence: 99%

Are the Organellar Genomes Useful for Fine Scale Population Structure Analysis of Endangered Plants?—A Case Study of Pulsatilla patens (L.) Mill

Szandar

Sawicki

Paukszto

et al. 2022

Genes

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State of the population of Pulsatilla patens (L.) Mill. on the territorу National Nature Park «Podilski Tovtry»

Horbniak-Yulina

2024

Proc. SNHM

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The population of Pulsatilla patens has been described in the NNP «Podilski Tovtry» national nature park on the territory of the «Ivakhnovetskiy» protected area. The article analyzes the data from the literature on the possible systematic position of P. patens and its range. Literary sources and herbarium specimens stored in local herbaria were processed, and probable localities in the territory of the national park were described. The only place of growth on the «Ivakhnovetskiy» of the NNP «Podilski Tovtry» has been confirmed. The ecological and coenotic relationships are characterized, and the biological features of the studied species are discussed. The geobotanical descriptions within the «Ivakhnovetskiy» protected area are carried out. The habitats and communities from the Green Book of Ukraine with P. patens were identified. The physical and geographical features of the habitat of the studied species are characterized. The results of statistical processing of morphometric parameters of P. patens, which were analyzed within the NNP «Podilski Tovtry», are processed and presented. It was found that the population on the «Ivakhnovetskiy» is predominantly homogeneous. The largest coefficient of variation was determined for the number of flowers and the height of the pedunculate shoot, which is due to the biological characteristics of the species. Based on the results of accounting for the age structure of the P. patens population in this locality, it was found that the recovery index is moderate. The factors of influence on the population of Pulsatilla patens on the «Ivakhnovetskiy» were determined. The conservation measures for the restoration and conservation of the rare species P. patens in the territory of NNP «Podilski Tovtry» and in Ukraine in particular at the eastern limit of its distribution are proposed. It is proposed to propagate the germinated seeds at the collection site with further return to the natural habitat of «Ivakhnovetskiy» and other localities identified during the study of literary and herbarium materials – to reintroduce it. Controlled spontaneous succession in abandoned and/or still active limestone quarries with large areas devoid of dense vegetation can be an effective tool for population recovery.

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Re-introduction of an extinct population of Pulsatilla patens using different propagation techniques

Cited by 2 publications

References 42 publications

Are the Organellar Genomes Useful for Fine Scale Population Structure Analysis of Endangered Plants?—A Case Study of Pulsatilla patens (L.) Mill

Are the Organellar Genomes Useful for Fine Scale Population Structure Analysis of Endangered Plants?—A Case Study of Pulsatilla patens (L.) Mill

State of the population of Pulsatilla patens (L.) Mill. on the territorу National Nature Park «Podilski Tovtry»

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